Limits...
Influence of hyperprolactinemia on collagen fibers in the lacrimal gland of female mice.

Araujo AS, Simões Mde J, Verna C, Simões RS, Soares Júnior JM, Baracat EC, Gomes RC - Clinics (Sao Paulo) (2015)

Bottom Line: Morphological analysis revealed greater structural tissue disorganization of the lacrimal glands in the metoclopramide-treated groups.The total collagen content was significantly higher in the HPRL1 group than in the CTR1 group (p<0.05), whereas the difference between the CTR2 and HPRL2 groups was not significant.Our data suggest an impairment in the functioning of the lacrimal gland as a consequence of increased prolactin levels and decreased serum levels of estrogen and progesterone.

View Article: PubMed Central - PubMed

Affiliation: Morfologia e Genética, Universidade Federal de São Paulo, BR.

ABSTRACT

Objective: To quantify the collagen fibers in the lacrimal gland of female mice with hyperprolactinemia.

Methods: Forty adult female mice were randomly divided into two groups with 20 animals each: nonpregnant control (CTR1, control group, 0.2 mL of saline solution) and nonpregnant experimental (HPRL1, experimental group, 200 µg/day metoclopramide). Treatments lasted for 50 consecutive days. On day 50, 10 females from each group (control and experimental) were euthanized in the proestrus phase; then, the blood was collected and the lacrimal glands were removed. Thereafter, the remaining females were placed with the mates and continued to receive treatment with saline solution or metoclopramide. On the 6th post-coital day, 10 pregnant females from the control group (CTR2) and 10 pregnant females from the experimental group (HPRL2) were euthanized, after which blood was collected and the lacrimal glands removed. The lacrimal glands were processed for morphological analyses and collagen quantification, and prolactin and sex steroid levels were measured in the blood samples. Data were statistically analyzed using an unpaired Student t test (p<0.05).

Results: Morphological analysis revealed greater structural tissue disorganization of the lacrimal glands in the metoclopramide-treated groups. The total collagen content was significantly higher in the HPRL1 group than in the CTR1 group (p<0.05), whereas the difference between the CTR2 and HPRL2 groups was not significant.

Conclusion: Our data suggest an impairment in the functioning of the lacrimal gland as a consequence of increased prolactin levels and decreased serum levels of estrogen and progesterone.

No MeSH data available.


Related in: MedlinePlus

Photomicrographs of lacrimal glands in the nonpregnant groups (CTR1 and HPRL1) and the pregnant groups (CTR2 and HPRL2) after Masson's trichrome staining. Note the blue collagen fibers (arrow), the lumen (L) and the acinar cells (A). The bar corresponds to 400x magnification.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4557571&req=5

f1-cln_70p632: Photomicrographs of lacrimal glands in the nonpregnant groups (CTR1 and HPRL1) and the pregnant groups (CTR2 and HPRL2) after Masson's trichrome staining. Note the blue collagen fibers (arrow), the lumen (L) and the acinar cells (A). The bar corresponds to 400x magnification.

Mentions: The photomicrographs were used for the semiquantification of collagen fibers (blue) in the lacrimal glands using ImageLab® processing and image analysis software (Computer Software Ltd, Brazil). For each lacrimal gland, 10 histological fields per section were evaluated to cover the entire length of the cut, enabling a complete analysis. The analyses were performed by two independent researchers who were blinded to the sample identity. The calculations were repeated for each animal. The results are expressed as % collagen per μm2 (Figures 1 and 2).


Influence of hyperprolactinemia on collagen fibers in the lacrimal gland of female mice.

Araujo AS, Simões Mde J, Verna C, Simões RS, Soares Júnior JM, Baracat EC, Gomes RC - Clinics (Sao Paulo) (2015)

Photomicrographs of lacrimal glands in the nonpregnant groups (CTR1 and HPRL1) and the pregnant groups (CTR2 and HPRL2) after Masson's trichrome staining. Note the blue collagen fibers (arrow), the lumen (L) and the acinar cells (A). The bar corresponds to 400x magnification.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4557571&req=5

f1-cln_70p632: Photomicrographs of lacrimal glands in the nonpregnant groups (CTR1 and HPRL1) and the pregnant groups (CTR2 and HPRL2) after Masson's trichrome staining. Note the blue collagen fibers (arrow), the lumen (L) and the acinar cells (A). The bar corresponds to 400x magnification.
Mentions: The photomicrographs were used for the semiquantification of collagen fibers (blue) in the lacrimal glands using ImageLab® processing and image analysis software (Computer Software Ltd, Brazil). For each lacrimal gland, 10 histological fields per section were evaluated to cover the entire length of the cut, enabling a complete analysis. The analyses were performed by two independent researchers who were blinded to the sample identity. The calculations were repeated for each animal. The results are expressed as % collagen per μm2 (Figures 1 and 2).

Bottom Line: Morphological analysis revealed greater structural tissue disorganization of the lacrimal glands in the metoclopramide-treated groups.The total collagen content was significantly higher in the HPRL1 group than in the CTR1 group (p<0.05), whereas the difference between the CTR2 and HPRL2 groups was not significant.Our data suggest an impairment in the functioning of the lacrimal gland as a consequence of increased prolactin levels and decreased serum levels of estrogen and progesterone.

View Article: PubMed Central - PubMed

Affiliation: Morfologia e Genética, Universidade Federal de São Paulo, BR.

ABSTRACT

Objective: To quantify the collagen fibers in the lacrimal gland of female mice with hyperprolactinemia.

Methods: Forty adult female mice were randomly divided into two groups with 20 animals each: nonpregnant control (CTR1, control group, 0.2 mL of saline solution) and nonpregnant experimental (HPRL1, experimental group, 200 µg/day metoclopramide). Treatments lasted for 50 consecutive days. On day 50, 10 females from each group (control and experimental) were euthanized in the proestrus phase; then, the blood was collected and the lacrimal glands were removed. Thereafter, the remaining females were placed with the mates and continued to receive treatment with saline solution or metoclopramide. On the 6th post-coital day, 10 pregnant females from the control group (CTR2) and 10 pregnant females from the experimental group (HPRL2) were euthanized, after which blood was collected and the lacrimal glands removed. The lacrimal glands were processed for morphological analyses and collagen quantification, and prolactin and sex steroid levels were measured in the blood samples. Data were statistically analyzed using an unpaired Student t test (p<0.05).

Results: Morphological analysis revealed greater structural tissue disorganization of the lacrimal glands in the metoclopramide-treated groups. The total collagen content was significantly higher in the HPRL1 group than in the CTR1 group (p<0.05), whereas the difference between the CTR2 and HPRL2 groups was not significant.

Conclusion: Our data suggest an impairment in the functioning of the lacrimal gland as a consequence of increased prolactin levels and decreased serum levels of estrogen and progesterone.

No MeSH data available.


Related in: MedlinePlus