Limits...
A second component of the SltA-dependent cation tolerance pathway in Aspergillus nidulans.

Mellado L, Calcagno-Pizarelli AM, Lockington RA, Cortese MS, Kelly JM, Arst HN, Espeso EA - Fungal Genet. Biol. (2015)

Bottom Line: This functional relationship is reinforced by the fact that numerous mutations in both slt loci can be isolated as suppressors of poor colonial growth resulting from certain vps (vacuolar protein sorting) mutations.Finally, it is noteworthy that SltA and SltB have a similar, limited phylogenetic distribution, being restricted to the pezizomycotina subphylum.The relevance of the Slt regulatory pathway to cell structure, intracellular trafficking and cation homeostasis and its restricted phylogenetic distribution makes this pathway of general interest for future investigation and as a source of targets for antifungal drugs.

View Article: PubMed Central - PubMed

Affiliation: Departamento de Biología Celular y Molecular, Centro de Investigaciones Biológicas, CSIC, Ramiro de Maeztu, 9, 28040 Madrid, Spain.

No MeSH data available.


Related in: MedlinePlus

pskA, AN7985, does not play a role in the slt pathway. A. A  pskA strain has a wild-type phenotype under different cation and alkaline pH stress conditions. B. Absence of PskA protein does not suppress the debilitated growth resulting from a  vps3 allele. However, spontaneous suppressors of vps3Δ arise on homokaryon purification plates. Suppressor sectors 1 and 2, indicated with red arrowheads, were purified and further analyzed for the presence of mutations in sltA and sltB (see Table 2).
© Copyright Policy - CC BY
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4557415&req=5

f0065: pskA, AN7985, does not play a role in the slt pathway. A. A pskA strain has a wild-type phenotype under different cation and alkaline pH stress conditions. B. Absence of PskA protein does not suppress the debilitated growth resulting from a vps3 allele. However, spontaneous suppressors of vps3Δ arise on homokaryon purification plates. Suppressor sectors 1 and 2, indicated with red arrowheads, were purified and further analyzed for the presence of mutations in sltA and sltB (see Table 2).


A second component of the SltA-dependent cation tolerance pathway in Aspergillus nidulans.

Mellado L, Calcagno-Pizarelli AM, Lockington RA, Cortese MS, Kelly JM, Arst HN, Espeso EA - Fungal Genet. Biol. (2015)

pskA, AN7985, does not play a role in the slt pathway. A. A  pskA strain has a wild-type phenotype under different cation and alkaline pH stress conditions. B. Absence of PskA protein does not suppress the debilitated growth resulting from a  vps3 allele. However, spontaneous suppressors of vps3Δ arise on homokaryon purification plates. Suppressor sectors 1 and 2, indicated with red arrowheads, were purified and further analyzed for the presence of mutations in sltA and sltB (see Table 2).
© Copyright Policy - CC BY
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4557415&req=5

f0065: pskA, AN7985, does not play a role in the slt pathway. A. A pskA strain has a wild-type phenotype under different cation and alkaline pH stress conditions. B. Absence of PskA protein does not suppress the debilitated growth resulting from a vps3 allele. However, spontaneous suppressors of vps3Δ arise on homokaryon purification plates. Suppressor sectors 1 and 2, indicated with red arrowheads, were purified and further analyzed for the presence of mutations in sltA and sltB (see Table 2).
Bottom Line: This functional relationship is reinforced by the fact that numerous mutations in both slt loci can be isolated as suppressors of poor colonial growth resulting from certain vps (vacuolar protein sorting) mutations.Finally, it is noteworthy that SltA and SltB have a similar, limited phylogenetic distribution, being restricted to the pezizomycotina subphylum.The relevance of the Slt regulatory pathway to cell structure, intracellular trafficking and cation homeostasis and its restricted phylogenetic distribution makes this pathway of general interest for future investigation and as a source of targets for antifungal drugs.

View Article: PubMed Central - PubMed

Affiliation: Departamento de Biología Celular y Molecular, Centro de Investigaciones Biológicas, CSIC, Ramiro de Maeztu, 9, 28040 Madrid, Spain.

No MeSH data available.


Related in: MedlinePlus