Limits...
Analysis of cytochrome b(5) reductase-mediated metabolism in the phytopathogenic fungus Zymoseptoria tritici reveals novel functionalities implicated in virulence.

Derbyshire MC, Michaelson L, Parker J, Kelly S, Thacker U, Powers SJ, Bailey A, Hammond-Kosack K, Courbot M, Rudd J - Fungal Genet. Biol. (2015)

Bottom Line: These defects coincided with alterations in fatty acid, sphingolipid and sterol biosynthesis observed through GC-MS and HPLC analyses.Our study reports the first functional characterisation of CBR gene family members in a plant pathogenic filamentous fungus.This also represents the first direct observation of CBR functional ablation impacting upon fungal sterol biosynthesis.

View Article: PubMed Central - PubMed

Affiliation: Department of Plant Biology and Crop Science, Rothamsted Research, West Common, Harpenden, Hertfordshire AL5 2JQ, UK. Electronic address: markcharder@gmail.com.

No MeSH data available.


Related in: MedlinePlus

ΔZtCBR1 mutants have altered fatty acid methyl ester (FAME) profiles. (A) Mean relative abundance of the fatty acid methyl ester (FAME) species 16:0, 16:1, 18:0, 18:1, 18:2 and 18:3 in the WT and a ΔZtCBR1 strain. Bars represent standard error (** p < 0.001). (B) Total FAME content of WT and the same ΔZtCBR1 strain expressed in *** μg mg−1 of fresh weight. Bars represent standard error.
© Copyright Policy - CC BY
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4557397&req=5

f0030: ΔZtCBR1 mutants have altered fatty acid methyl ester (FAME) profiles. (A) Mean relative abundance of the fatty acid methyl ester (FAME) species 16:0, 16:1, 18:0, 18:1, 18:2 and 18:3 in the WT and a ΔZtCBR1 strain. Bars represent standard error (** p < 0.001). (B) Total FAME content of WT and the same ΔZtCBR1 strain expressed in *** μg mg−1 of fresh weight. Bars represent standard error.

Mentions: In order to assess whether ZtCBR1 ablation had impacted on fatty acid biosynthesis by fungal cells, the fatty acid methyl ester (FAME) profile of the WT strain and ΔZtCBR1-1 were analysed using GC–MS. ΔZtCBR1-1 displayed a significant decrease in relative abundance of the fatty acid species 16:1, 18:0 and 18:1 (p < 0.001). This strain also displayed a significant increase in relative abundance of the polyunsaturated species 18:2 (p < 0.001). The relative abundances of the species 16:0 and 18:3 were not significantly changed relative to WT relative abundances (p > 0.05) (Fig. 6A). The total amount of FAMEs in ΔZtCBR1-1 was not significantly altered relative to WT levels (p > 0.05) (Fig. 6B).


Analysis of cytochrome b(5) reductase-mediated metabolism in the phytopathogenic fungus Zymoseptoria tritici reveals novel functionalities implicated in virulence.

Derbyshire MC, Michaelson L, Parker J, Kelly S, Thacker U, Powers SJ, Bailey A, Hammond-Kosack K, Courbot M, Rudd J - Fungal Genet. Biol. (2015)

ΔZtCBR1 mutants have altered fatty acid methyl ester (FAME) profiles. (A) Mean relative abundance of the fatty acid methyl ester (FAME) species 16:0, 16:1, 18:0, 18:1, 18:2 and 18:3 in the WT and a ΔZtCBR1 strain. Bars represent standard error (** p < 0.001). (B) Total FAME content of WT and the same ΔZtCBR1 strain expressed in *** μg mg−1 of fresh weight. Bars represent standard error.
© Copyright Policy - CC BY
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4557397&req=5

f0030: ΔZtCBR1 mutants have altered fatty acid methyl ester (FAME) profiles. (A) Mean relative abundance of the fatty acid methyl ester (FAME) species 16:0, 16:1, 18:0, 18:1, 18:2 and 18:3 in the WT and a ΔZtCBR1 strain. Bars represent standard error (** p < 0.001). (B) Total FAME content of WT and the same ΔZtCBR1 strain expressed in *** μg mg−1 of fresh weight. Bars represent standard error.
Mentions: In order to assess whether ZtCBR1 ablation had impacted on fatty acid biosynthesis by fungal cells, the fatty acid methyl ester (FAME) profile of the WT strain and ΔZtCBR1-1 were analysed using GC–MS. ΔZtCBR1-1 displayed a significant decrease in relative abundance of the fatty acid species 16:1, 18:0 and 18:1 (p < 0.001). This strain also displayed a significant increase in relative abundance of the polyunsaturated species 18:2 (p < 0.001). The relative abundances of the species 16:0 and 18:3 were not significantly changed relative to WT relative abundances (p > 0.05) (Fig. 6A). The total amount of FAMEs in ΔZtCBR1-1 was not significantly altered relative to WT levels (p > 0.05) (Fig. 6B).

Bottom Line: These defects coincided with alterations in fatty acid, sphingolipid and sterol biosynthesis observed through GC-MS and HPLC analyses.Our study reports the first functional characterisation of CBR gene family members in a plant pathogenic filamentous fungus.This also represents the first direct observation of CBR functional ablation impacting upon fungal sterol biosynthesis.

View Article: PubMed Central - PubMed

Affiliation: Department of Plant Biology and Crop Science, Rothamsted Research, West Common, Harpenden, Hertfordshire AL5 2JQ, UK. Electronic address: markcharder@gmail.com.

No MeSH data available.


Related in: MedlinePlus