Limits...
Analysis of cytochrome b(5) reductase-mediated metabolism in the phytopathogenic fungus Zymoseptoria tritici reveals novel functionalities implicated in virulence.

Derbyshire MC, Michaelson L, Parker J, Kelly S, Thacker U, Powers SJ, Bailey A, Hammond-Kosack K, Courbot M, Rudd J - Fungal Genet. Biol. (2015)

Bottom Line: Microsomal cytochrome b5 reductases (CBRs) are an important family of electron transfer proteins which in eukaryotes are involved in the biosynthesis of fatty acids and complex lipids including sphingolipids and sterols.Our study reports the first functional characterisation of CBR gene family members in a plant pathogenic filamentous fungus.This also represents the first direct observation of CBR functional ablation impacting upon fungal sterol biosynthesis.

View Article: PubMed Central - PubMed

Affiliation: Department of Plant Biology and Crop Science, Rothamsted Research, West Common, Harpenden, Hertfordshire AL5 2JQ, UK. Electronic address: markcharder@gmail.com.

No MeSH data available.


Related in: MedlinePlus

ΔZtCBR1 mutants have altered fatty acid methyl ester (FAME) profiles. (A) Mean relative abundance of the fatty acid methyl ester (FAME) species 16:0, 16:1, 18:0, 18:1, 18:2 and 18:3 in the WT and a ΔZtCBR1 strain. Bars represent standard error (** p < 0.001). (B) Total FAME content of WT and the same ΔZtCBR1 strain expressed in *** μg mg−1 of fresh weight. Bars represent standard error.
© Copyright Policy - CC BY
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4557397&req=5

f0030: ΔZtCBR1 mutants have altered fatty acid methyl ester (FAME) profiles. (A) Mean relative abundance of the fatty acid methyl ester (FAME) species 16:0, 16:1, 18:0, 18:1, 18:2 and 18:3 in the WT and a ΔZtCBR1 strain. Bars represent standard error (** p < 0.001). (B) Total FAME content of WT and the same ΔZtCBR1 strain expressed in *** μg mg−1 of fresh weight. Bars represent standard error.

Mentions: In order to assess whether ZtCBR1 ablation had impacted on fatty acid biosynthesis by fungal cells, the fatty acid methyl ester (FAME) profile of the WT strain and ΔZtCBR1-1 were analysed using GC–MS. ΔZtCBR1-1 displayed a significant decrease in relative abundance of the fatty acid species 16:1, 18:0 and 18:1 (p < 0.001). This strain also displayed a significant increase in relative abundance of the polyunsaturated species 18:2 (p < 0.001). The relative abundances of the species 16:0 and 18:3 were not significantly changed relative to WT relative abundances (p > 0.05) (Fig. 6A). The total amount of FAMEs in ΔZtCBR1-1 was not significantly altered relative to WT levels (p > 0.05) (Fig. 6B).


Analysis of cytochrome b(5) reductase-mediated metabolism in the phytopathogenic fungus Zymoseptoria tritici reveals novel functionalities implicated in virulence.

Derbyshire MC, Michaelson L, Parker J, Kelly S, Thacker U, Powers SJ, Bailey A, Hammond-Kosack K, Courbot M, Rudd J - Fungal Genet. Biol. (2015)

ΔZtCBR1 mutants have altered fatty acid methyl ester (FAME) profiles. (A) Mean relative abundance of the fatty acid methyl ester (FAME) species 16:0, 16:1, 18:0, 18:1, 18:2 and 18:3 in the WT and a ΔZtCBR1 strain. Bars represent standard error (** p < 0.001). (B) Total FAME content of WT and the same ΔZtCBR1 strain expressed in *** μg mg−1 of fresh weight. Bars represent standard error.
© Copyright Policy - CC BY
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4557397&req=5

f0030: ΔZtCBR1 mutants have altered fatty acid methyl ester (FAME) profiles. (A) Mean relative abundance of the fatty acid methyl ester (FAME) species 16:0, 16:1, 18:0, 18:1, 18:2 and 18:3 in the WT and a ΔZtCBR1 strain. Bars represent standard error (** p < 0.001). (B) Total FAME content of WT and the same ΔZtCBR1 strain expressed in *** μg mg−1 of fresh weight. Bars represent standard error.
Mentions: In order to assess whether ZtCBR1 ablation had impacted on fatty acid biosynthesis by fungal cells, the fatty acid methyl ester (FAME) profile of the WT strain and ΔZtCBR1-1 were analysed using GC–MS. ΔZtCBR1-1 displayed a significant decrease in relative abundance of the fatty acid species 16:1, 18:0 and 18:1 (p < 0.001). This strain also displayed a significant increase in relative abundance of the polyunsaturated species 18:2 (p < 0.001). The relative abundances of the species 16:0 and 18:3 were not significantly changed relative to WT relative abundances (p > 0.05) (Fig. 6A). The total amount of FAMEs in ΔZtCBR1-1 was not significantly altered relative to WT levels (p > 0.05) (Fig. 6B).

Bottom Line: Microsomal cytochrome b5 reductases (CBRs) are an important family of electron transfer proteins which in eukaryotes are involved in the biosynthesis of fatty acids and complex lipids including sphingolipids and sterols.Our study reports the first functional characterisation of CBR gene family members in a plant pathogenic filamentous fungus.This also represents the first direct observation of CBR functional ablation impacting upon fungal sterol biosynthesis.

View Article: PubMed Central - PubMed

Affiliation: Department of Plant Biology and Crop Science, Rothamsted Research, West Common, Harpenden, Hertfordshire AL5 2JQ, UK. Electronic address: markcharder@gmail.com.

No MeSH data available.


Related in: MedlinePlus