Limits...
A piRNA-like small RNA interacts with and modulates p-ERM proteins in human somatic cells.

Mei Y, Wang Y, Kumari P, Shetty AC, Clark D, Gable T, MacKerell AD, Ma MZ, Weber DJ, Yang AJ, Edelman MJ, Mao L - Nat Commun (2015)

Bottom Line: However, the roles of piRNAs in somatic tissues are largely unknown.The piR-L-163/p-ERM interaction is critical for p-ERM's binding capability to filamentous actin (F-actin) and ERM-binding phosphoprotein 50 (EBP50).Thus, piRNA/piRNA-L may play a regulatory role through direct interaction with proteins in physiological and pathophysiological conditions.

View Article: PubMed Central - PubMed

Affiliation: Department of Oncology and Diagnostic Sciences, University of Maryland School of Dentistry, 650W Baltimore Street, Baltimore, Maryland 21201, USA.

ABSTRACT
PIWI-interacting RNAs (piRNAs) are thought to silence transposon and gene expression during development. However, the roles of piRNAs in somatic tissues are largely unknown. Here we report the identification of 555 piRNAs in human lung bronchial epithelial (HBE) and non-small cell lung cancer (NSCLC) cell lines, including 295 that do not exist in databases termed as piRNA-like sncRNAs or piRNA-Ls. Distinctive piRNA/piRNA-L expression patterns are observed between HBE and NSCLC cells. piRNA-like-163 (piR-L-163), the top downregulated piRNA-L in NSCLC cells, binds directly to phosphorylated ERM proteins (p-ERM), which is dependent on the central part of UUNNUUUNNUU motif in piR-L-163 and the RRRKPDT element in ERM. The piR-L-163/p-ERM interaction is critical for p-ERM's binding capability to filamentous actin (F-actin) and ERM-binding phosphoprotein 50 (EBP50). Thus, piRNA/piRNA-L may play a regulatory role through direct interaction with proteins in physiological and pathophysiological conditions.

No MeSH data available.


Related in: MedlinePlus

Flowchart of the protocol used to prepare sncRNAs from the cell lines for RNA sequencing.(a) Outlined steps of the preparation. (b) Size-guided sncRNA extraction at 1 nt resolution. (c) Secondary sncRNA purification after library construction based on sizes for RNA sequencing. The smaller sized products (low band) are likely microRNAs.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4557300&req=5

f1: Flowchart of the protocol used to prepare sncRNAs from the cell lines for RNA sequencing.(a) Outlined steps of the preparation. (b) Size-guided sncRNA extraction at 1 nt resolution. (c) Secondary sncRNA purification after library construction based on sizes for RNA sequencing. The smaller sized products (low band) are likely microRNAs.

Mentions: To explore potential implications of piRNA in lung cancer, we first analysed global piRNA expression profiles in eight non-small cell lung cancer (NSCLC) and three HBE cell lines. Small RNAs ranging approximately from 25 to 33 bases were used for library construction (Fig. 1a–c and Table 1) based on previous reports272829303132. RNA sequencing was performed and resulted in ∼4.5 million reads with >99% of the reads between 26 and 32 bases (Supplementary Fig. 1a and Supplementary Table 1), and ∼50% of the reads mapped to ≥2 loci in the human genome sequences (Supplementary Fig. 1b), indicating that the piRNA reads captured a nontrivial portion of piRNA diversity33.


A piRNA-like small RNA interacts with and modulates p-ERM proteins in human somatic cells.

Mei Y, Wang Y, Kumari P, Shetty AC, Clark D, Gable T, MacKerell AD, Ma MZ, Weber DJ, Yang AJ, Edelman MJ, Mao L - Nat Commun (2015)

Flowchart of the protocol used to prepare sncRNAs from the cell lines for RNA sequencing.(a) Outlined steps of the preparation. (b) Size-guided sncRNA extraction at 1 nt resolution. (c) Secondary sncRNA purification after library construction based on sizes for RNA sequencing. The smaller sized products (low band) are likely microRNAs.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4557300&req=5

f1: Flowchart of the protocol used to prepare sncRNAs from the cell lines for RNA sequencing.(a) Outlined steps of the preparation. (b) Size-guided sncRNA extraction at 1 nt resolution. (c) Secondary sncRNA purification after library construction based on sizes for RNA sequencing. The smaller sized products (low band) are likely microRNAs.
Mentions: To explore potential implications of piRNA in lung cancer, we first analysed global piRNA expression profiles in eight non-small cell lung cancer (NSCLC) and three HBE cell lines. Small RNAs ranging approximately from 25 to 33 bases were used for library construction (Fig. 1a–c and Table 1) based on previous reports272829303132. RNA sequencing was performed and resulted in ∼4.5 million reads with >99% of the reads between 26 and 32 bases (Supplementary Fig. 1a and Supplementary Table 1), and ∼50% of the reads mapped to ≥2 loci in the human genome sequences (Supplementary Fig. 1b), indicating that the piRNA reads captured a nontrivial portion of piRNA diversity33.

Bottom Line: However, the roles of piRNAs in somatic tissues are largely unknown.The piR-L-163/p-ERM interaction is critical for p-ERM's binding capability to filamentous actin (F-actin) and ERM-binding phosphoprotein 50 (EBP50).Thus, piRNA/piRNA-L may play a regulatory role through direct interaction with proteins in physiological and pathophysiological conditions.

View Article: PubMed Central - PubMed

Affiliation: Department of Oncology and Diagnostic Sciences, University of Maryland School of Dentistry, 650W Baltimore Street, Baltimore, Maryland 21201, USA.

ABSTRACT
PIWI-interacting RNAs (piRNAs) are thought to silence transposon and gene expression during development. However, the roles of piRNAs in somatic tissues are largely unknown. Here we report the identification of 555 piRNAs in human lung bronchial epithelial (HBE) and non-small cell lung cancer (NSCLC) cell lines, including 295 that do not exist in databases termed as piRNA-like sncRNAs or piRNA-Ls. Distinctive piRNA/piRNA-L expression patterns are observed between HBE and NSCLC cells. piRNA-like-163 (piR-L-163), the top downregulated piRNA-L in NSCLC cells, binds directly to phosphorylated ERM proteins (p-ERM), which is dependent on the central part of UUNNUUUNNUU motif in piR-L-163 and the RRRKPDT element in ERM. The piR-L-163/p-ERM interaction is critical for p-ERM's binding capability to filamentous actin (F-actin) and ERM-binding phosphoprotein 50 (EBP50). Thus, piRNA/piRNA-L may play a regulatory role through direct interaction with proteins in physiological and pathophysiological conditions.

No MeSH data available.


Related in: MedlinePlus