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Effect of mesenchymal stem cells transplantation on glycaemic profile & their localization in streptozotocin induced diabetic Wistar rats.

Bhansali S, Kumar V, Saikia UN, Medhi B, Jha V, Bhansali A, Dutta P - Indian J. Med. Res. (2015)

Bottom Line: Administration of mesenchymal stem cells (MSCs) in irradiated diabetic rat model has transiently shown to decrease blood glucose level.There was a significant reduction in blood glucose level after administration of MSCs in the experimental group (P<0.001).Co-expression of anti-BrdU and anti-insulin antibody indicated trans-differentiation / fusion into insulin producing cells evidenced by significant increase in total number of islet (P=0.004) and insulin positive cells ( P<0.0001) in experimental group.

View Article: PubMed Central - PubMed

Affiliation: Department of Endocrinology, Postgraduate Institute of Medical Education & Research, Chandigarh, India.

ABSTRACT

Background & objectives: Bone marrow is a rich source of adult stem cells that can differentiate into various cell types. Administration of mesenchymal stem cells (MSCs) in irradiated diabetic rat model has transiently shown to decrease blood glucose level. This study examines the effect of high dose and multiple injections of MSCs on glycemic profile, their localization and regeneration of islet in diabetic Wistar rat.

Methods: The study was carried out in male Wistar rats categorized into three groups (n=6, in each group): Group 1 as control, group 2 streptozotocin (STZ) (50 mg/kg) induced diabetic group and group 3 experimental group; 5-bromo-2-deoxyuridine (BrdU) labelled allogenic MSCs were injected in the non-irradiated diabetic rat of the experimental group through tail vein. The blood glucose profile was subsequently monitored at regular intervals. Rats were sacrificed on day 45 and pancreas was examined for localization of BrdU labelled stem cells by immunofluorescence and islet-neogenesis by immunohistochemistry .

Results: There was a significant reduction in blood glucose level after administration of MSCs in the experimental group (P<0.001). The presence of BrdU labelled MSCs in islet suggested their localization in the pancreas. Co-expression of anti-BrdU and anti-insulin antibody indicated trans-differentiation / fusion into insulin producing cells evidenced by significant increase in total number of islet (P=0.004) and insulin positive cells ( P<0.0001) in experimental group.

Interpretation & conclusions: Our results showed that the MSCs administration in non-irradiated diabetic Wistar rat reduced hyperglycaemia and was accompanied by increased islet-neogenesis, possibly through trans-differentiation/fusion.

No MeSH data available.


Related in: MedlinePlus

(A). Histology of the rat pancreas from control group, streptozotocin induced diabetic group and experimental group showing pancreatic islets at a magnification of 20X. H&E (arrows), (B) insulin reactivity in pancreatic tissue (arrow) shown by immunohistochemistry, and (C). immunofluorescence.
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Figure 3: (A). Histology of the rat pancreas from control group, streptozotocin induced diabetic group and experimental group showing pancreatic islets at a magnification of 20X. H&E (arrows), (B) insulin reactivity in pancreatic tissue (arrow) shown by immunohistochemistry, and (C). immunofluorescence.

Mentions: Histological assessment of the pancreatic tissue stained with haematoxylin and eosin showed destruction of islet tissue and low insulin reactivity on immunohistochemistry and immunofluorescence in the diabetic group as compared with the control group. Treatment with MSCs in diabetic Wistar rats resulted in reorganization of islet and partial restoration of β-cell as indicated by high insulin reactivity compared with the STZ induced diabetic group as shown in Fig. 3A, B and C. There was a significant increase in number of islet and insulin positive cells the experimental group as compared to the STZ induced diabetic group as shown on immunohistochemistry (P<0.001 and P<0.01) (Table).


Effect of mesenchymal stem cells transplantation on glycaemic profile & their localization in streptozotocin induced diabetic Wistar rats.

Bhansali S, Kumar V, Saikia UN, Medhi B, Jha V, Bhansali A, Dutta P - Indian J. Med. Res. (2015)

(A). Histology of the rat pancreas from control group, streptozotocin induced diabetic group and experimental group showing pancreatic islets at a magnification of 20X. H&E (arrows), (B) insulin reactivity in pancreatic tissue (arrow) shown by immunohistochemistry, and (C). immunofluorescence.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4557252&req=5

Figure 3: (A). Histology of the rat pancreas from control group, streptozotocin induced diabetic group and experimental group showing pancreatic islets at a magnification of 20X. H&E (arrows), (B) insulin reactivity in pancreatic tissue (arrow) shown by immunohistochemistry, and (C). immunofluorescence.
Mentions: Histological assessment of the pancreatic tissue stained with haematoxylin and eosin showed destruction of islet tissue and low insulin reactivity on immunohistochemistry and immunofluorescence in the diabetic group as compared with the control group. Treatment with MSCs in diabetic Wistar rats resulted in reorganization of islet and partial restoration of β-cell as indicated by high insulin reactivity compared with the STZ induced diabetic group as shown in Fig. 3A, B and C. There was a significant increase in number of islet and insulin positive cells the experimental group as compared to the STZ induced diabetic group as shown on immunohistochemistry (P<0.001 and P<0.01) (Table).

Bottom Line: Administration of mesenchymal stem cells (MSCs) in irradiated diabetic rat model has transiently shown to decrease blood glucose level.There was a significant reduction in blood glucose level after administration of MSCs in the experimental group (P<0.001).Co-expression of anti-BrdU and anti-insulin antibody indicated trans-differentiation / fusion into insulin producing cells evidenced by significant increase in total number of islet (P=0.004) and insulin positive cells ( P<0.0001) in experimental group.

View Article: PubMed Central - PubMed

Affiliation: Department of Endocrinology, Postgraduate Institute of Medical Education & Research, Chandigarh, India.

ABSTRACT

Background & objectives: Bone marrow is a rich source of adult stem cells that can differentiate into various cell types. Administration of mesenchymal stem cells (MSCs) in irradiated diabetic rat model has transiently shown to decrease blood glucose level. This study examines the effect of high dose and multiple injections of MSCs on glycemic profile, their localization and regeneration of islet in diabetic Wistar rat.

Methods: The study was carried out in male Wistar rats categorized into three groups (n=6, in each group): Group 1 as control, group 2 streptozotocin (STZ) (50 mg/kg) induced diabetic group and group 3 experimental group; 5-bromo-2-deoxyuridine (BrdU) labelled allogenic MSCs were injected in the non-irradiated diabetic rat of the experimental group through tail vein. The blood glucose profile was subsequently monitored at regular intervals. Rats were sacrificed on day 45 and pancreas was examined for localization of BrdU labelled stem cells by immunofluorescence and islet-neogenesis by immunohistochemistry .

Results: There was a significant reduction in blood glucose level after administration of MSCs in the experimental group (P<0.001). The presence of BrdU labelled MSCs in islet suggested their localization in the pancreas. Co-expression of anti-BrdU and anti-insulin antibody indicated trans-differentiation / fusion into insulin producing cells evidenced by significant increase in total number of islet (P=0.004) and insulin positive cells ( P<0.0001) in experimental group.

Interpretation & conclusions: Our results showed that the MSCs administration in non-irradiated diabetic Wistar rat reduced hyperglycaemia and was accompanied by increased islet-neogenesis, possibly through trans-differentiation/fusion.

No MeSH data available.


Related in: MedlinePlus