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Dopamine D1 receptor activation regulates the expression of the estrogen synthesis gene aromatase B in radial glial cells.

Xing L, McDonald H, Da Fonte DF, Gutierrez-Villagomez JM, Trudeau VL - Front Neurosci (2015)

Bottom Line: The estrogen synthesis enzyme aromatase B is exclusively found in RGCs, but this is lost as cells differentiate to neurons and other glial types in adult teleost brain.Pharmacological experiments using the cultured RGCs established that specific activation of dopamine D1 receptors up-regulates aromatase B mRNA through a cyclic adenosine monophosphate-dependent molecular mechanism.These data indicate that dopamine enhances the steroidogenic function of this neuronal progenitor cell.

View Article: PubMed Central - PubMed

Affiliation: Department of Biology, Centre for Advanced Research in Environmental Genomics, University of Ottawa Ottawa, ON, Canada.

ABSTRACT
Radial glial cells (RGCs) are abundant stem-like non-neuronal progenitors that are important for adult neurogenesis and brain repair, yet little is known about their regulation by neurotransmitters. Here we provide evidence for neuronal-glial interactions via a novel role for dopamine to stimulate RGC function. Goldfish were chosen as the model organism due to the abundance of RGCs and regenerative abilities of the adult central nervous system. A close anatomical relationship was observed between tyrosine hydroxylase-positive catecholaminergic cell bodies and axons and dopamine-D1 receptor expressing RGCs along the ventricular surface of telencephalon, a site of active neurogenesis. A primary cell culture model was established and immunofluorescence analysis indicates that in vitro RGCs from female goldfish retain their major characteristics in vivo, including expression of glial fibrillary acidic protein and brain lipid binding protein. The estrogen synthesis enzyme aromatase B is exclusively found in RGCs, but this is lost as cells differentiate to neurons and other glial types in adult teleost brain. Pharmacological experiments using the cultured RGCs established that specific activation of dopamine D1 receptors up-regulates aromatase B mRNA through a cyclic adenosine monophosphate-dependent molecular mechanism. These data indicate that dopamine enhances the steroidogenic function of this neuronal progenitor cell.

No MeSH data available.


Double fluorescence detection of GFAP (red) and D1R (green) in ventral telencephalic area of female goldfish brain. The confocal image shows that RGCs marked by GFAP are located at the ventricular surface (A,E) with fibers extending to the area ventralis telencephali pars ventralis (I) and the expression of D1R at the ventricular surface and the area ventralis telencephali pars ventralis (B,F,J). The expression of D1R by RGCs is shown in the merged images (V; ventricle, D,H,L). Magnification of the boxed area 1 in (D) shows that D1R is expressed by RGCs along the ventricular surface (H). Magnification of the boxed area 2 in (D) shows that D1R is expressed by RGCs in the area ventralis telencephali pars ventralis (L). Single slice scanning view of the boxed area 3 in panel H shows the colocalization of D1R and GFAP in RGCs along the ventricular surface (M). Slice scan view of the boxed area 4 in (L) shows the colocalization of D1R and GFAP in RGCs in the area ventralis telencephali pars ventralis (N). The nuclear stain DAPI (blue) is also shown (C,G,K). Scale bar = 20 μm in (A–L); Scale bar = 5 μm in (M,N).
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Figure 3: Double fluorescence detection of GFAP (red) and D1R (green) in ventral telencephalic area of female goldfish brain. The confocal image shows that RGCs marked by GFAP are located at the ventricular surface (A,E) with fibers extending to the area ventralis telencephali pars ventralis (I) and the expression of D1R at the ventricular surface and the area ventralis telencephali pars ventralis (B,F,J). The expression of D1R by RGCs is shown in the merged images (V; ventricle, D,H,L). Magnification of the boxed area 1 in (D) shows that D1R is expressed by RGCs along the ventricular surface (H). Magnification of the boxed area 2 in (D) shows that D1R is expressed by RGCs in the area ventralis telencephali pars ventralis (L). Single slice scanning view of the boxed area 3 in panel H shows the colocalization of D1R and GFAP in RGCs along the ventricular surface (M). Slice scan view of the boxed area 4 in (L) shows the colocalization of D1R and GFAP in RGCs in the area ventralis telencephali pars ventralis (N). The nuclear stain DAPI (blue) is also shown (C,G,K). Scale bar = 20 μm in (A–L); Scale bar = 5 μm in (M,N).

Mentions: The presence of DA receptors on post-synaptic cells is required for direct regulation upon the release of DA by TH-positive catecholaminergic neurons. Both GFAP (Figure 3A) and dopamine D1 receptor (D1R) (Figure 3B)-positive staining can be observed along the ventricular surface (Figures 3E,F), and in the area ventralis telencephali pars ventralis (Figures 3I,J). The merged confocal images show the colocalization of GFAP and D1R immunoreactivity (Figures 3C,D,G,H,K–N), which indicates that RGCs express D1R. See Supplemental Figure 1 for validation of the D1R antibody (Supplemental Figure 1).


Dopamine D1 receptor activation regulates the expression of the estrogen synthesis gene aromatase B in radial glial cells.

Xing L, McDonald H, Da Fonte DF, Gutierrez-Villagomez JM, Trudeau VL - Front Neurosci (2015)

Double fluorescence detection of GFAP (red) and D1R (green) in ventral telencephalic area of female goldfish brain. The confocal image shows that RGCs marked by GFAP are located at the ventricular surface (A,E) with fibers extending to the area ventralis telencephali pars ventralis (I) and the expression of D1R at the ventricular surface and the area ventralis telencephali pars ventralis (B,F,J). The expression of D1R by RGCs is shown in the merged images (V; ventricle, D,H,L). Magnification of the boxed area 1 in (D) shows that D1R is expressed by RGCs along the ventricular surface (H). Magnification of the boxed area 2 in (D) shows that D1R is expressed by RGCs in the area ventralis telencephali pars ventralis (L). Single slice scanning view of the boxed area 3 in panel H shows the colocalization of D1R and GFAP in RGCs along the ventricular surface (M). Slice scan view of the boxed area 4 in (L) shows the colocalization of D1R and GFAP in RGCs in the area ventralis telencephali pars ventralis (N). The nuclear stain DAPI (blue) is also shown (C,G,K). Scale bar = 20 μm in (A–L); Scale bar = 5 μm in (M,N).
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Related In: Results  -  Collection

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Figure 3: Double fluorescence detection of GFAP (red) and D1R (green) in ventral telencephalic area of female goldfish brain. The confocal image shows that RGCs marked by GFAP are located at the ventricular surface (A,E) with fibers extending to the area ventralis telencephali pars ventralis (I) and the expression of D1R at the ventricular surface and the area ventralis telencephali pars ventralis (B,F,J). The expression of D1R by RGCs is shown in the merged images (V; ventricle, D,H,L). Magnification of the boxed area 1 in (D) shows that D1R is expressed by RGCs along the ventricular surface (H). Magnification of the boxed area 2 in (D) shows that D1R is expressed by RGCs in the area ventralis telencephali pars ventralis (L). Single slice scanning view of the boxed area 3 in panel H shows the colocalization of D1R and GFAP in RGCs along the ventricular surface (M). Slice scan view of the boxed area 4 in (L) shows the colocalization of D1R and GFAP in RGCs in the area ventralis telencephali pars ventralis (N). The nuclear stain DAPI (blue) is also shown (C,G,K). Scale bar = 20 μm in (A–L); Scale bar = 5 μm in (M,N).
Mentions: The presence of DA receptors on post-synaptic cells is required for direct regulation upon the release of DA by TH-positive catecholaminergic neurons. Both GFAP (Figure 3A) and dopamine D1 receptor (D1R) (Figure 3B)-positive staining can be observed along the ventricular surface (Figures 3E,F), and in the area ventralis telencephali pars ventralis (Figures 3I,J). The merged confocal images show the colocalization of GFAP and D1R immunoreactivity (Figures 3C,D,G,H,K–N), which indicates that RGCs express D1R. See Supplemental Figure 1 for validation of the D1R antibody (Supplemental Figure 1).

Bottom Line: The estrogen synthesis enzyme aromatase B is exclusively found in RGCs, but this is lost as cells differentiate to neurons and other glial types in adult teleost brain.Pharmacological experiments using the cultured RGCs established that specific activation of dopamine D1 receptors up-regulates aromatase B mRNA through a cyclic adenosine monophosphate-dependent molecular mechanism.These data indicate that dopamine enhances the steroidogenic function of this neuronal progenitor cell.

View Article: PubMed Central - PubMed

Affiliation: Department of Biology, Centre for Advanced Research in Environmental Genomics, University of Ottawa Ottawa, ON, Canada.

ABSTRACT
Radial glial cells (RGCs) are abundant stem-like non-neuronal progenitors that are important for adult neurogenesis and brain repair, yet little is known about their regulation by neurotransmitters. Here we provide evidence for neuronal-glial interactions via a novel role for dopamine to stimulate RGC function. Goldfish were chosen as the model organism due to the abundance of RGCs and regenerative abilities of the adult central nervous system. A close anatomical relationship was observed between tyrosine hydroxylase-positive catecholaminergic cell bodies and axons and dopamine-D1 receptor expressing RGCs along the ventricular surface of telencephalon, a site of active neurogenesis. A primary cell culture model was established and immunofluorescence analysis indicates that in vitro RGCs from female goldfish retain their major characteristics in vivo, including expression of glial fibrillary acidic protein and brain lipid binding protein. The estrogen synthesis enzyme aromatase B is exclusively found in RGCs, but this is lost as cells differentiate to neurons and other glial types in adult teleost brain. Pharmacological experiments using the cultured RGCs established that specific activation of dopamine D1 receptors up-regulates aromatase B mRNA through a cyclic adenosine monophosphate-dependent molecular mechanism. These data indicate that dopamine enhances the steroidogenic function of this neuronal progenitor cell.

No MeSH data available.