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The protective effects of oral low-dose quercetin on diabetic nephropathy in hypercholesterolemic mice.

Gomes IB, Porto ML, Santos MC, Campagnaro BP, Gava AL, Meyrelles SS, Pereira TM, Vasquez EC - Front Physiol (2015)

Bottom Line: We also observed protective effects on the renal structural changes, including normalization of the index of glomerulosclerosis and kidney weight/body weight.Our data revealed that quercetin treatment significantly reduced DN in hypercholesterolemic mice by inducing biochemical changes (decrease in glucose and triglycerides serum levels) and reduction of glomerulosclerosis.Thus, this study highlights the relevance of quercetin as an alternative therapeutic option for DN, including in diabetes associated with dyslipidemia.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Translational Physiology, Health Sciences Center, Federal University of Espirito Santo Vitoria, Brazil.

ABSTRACT

Aims: Diabetic nephropathy (DN) is one of the most important causes of chronic renal disease, and the incidence of DN is increasing worldwide. Considering our previous report (Gomes et al., 2014) indicating that chronic treatment with oral low-dose quercetin (10 mg/Kg) demonstrated anti-oxidative, anti-apoptotic and renoprotective effects in the C57BL/6J model of DN, we investigated whether this flavonoid could also have beneficial effects in concurrent DN and spontaneous atherosclerosis using the apolipoprotein E-deficient mouse (apoE(-/-)).

Methods: Streptozotocin was used to induce diabetes (100 mg/kg/day, 3 days) in male apoE(-/-) mice (8 week-old). After 6 weeks, the mice were randomly separated into DQ: diabetic apoE(-/-) mice treated with quercetin (10 mg/kg/day, 4 weeks, n = 8), DV: diabetic ApoE(-/-) mice treated with vehicle (n = 8) and ND: non-treated non-diabetic mice (n = 8).

Results: Quercetin treatment diminished polyuria (~30%; p < 0.05), glycemia (~25%, p < 0.05), normalized the hypertriglyceridemia. Moreover, this bioflavonoid diminished creatininemia (~30%, p < 0.01) and reduced proteinuria but not to normal levels. We also observed protective effects on the renal structural changes, including normalization of the index of glomerulosclerosis and kidney weight/body weight.

Conclusions: Our data revealed that quercetin treatment significantly reduced DN in hypercholesterolemic mice by inducing biochemical changes (decrease in glucose and triglycerides serum levels) and reduction of glomerulosclerosis. Thus, this study highlights the relevance of quercetin as an alternative therapeutic option for DN, including in diabetes associated with dyslipidemia.

No MeSH data available.


Related in: MedlinePlus

Total plasma glucose (A), triglycerides (B), and cholesterol (C) in diabetic apoE−/− mice treated with quercetin (DQ) compared with diabetic apoE−/− mice administered vehicle (DV) compared with non-diabetic apoE−/− (ND) mice. Values are the means ± SEM for n = 6–8 mice per group. *p < 0.05 vs. ND, #p < 0.05 vs. DV.
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Figure 2: Total plasma glucose (A), triglycerides (B), and cholesterol (C) in diabetic apoE−/− mice treated with quercetin (DQ) compared with diabetic apoE−/− mice administered vehicle (DV) compared with non-diabetic apoE−/− (ND) mice. Values are the means ± SEM for n = 6–8 mice per group. *p < 0.05 vs. ND, #p < 0.05 vs. DV.

Mentions: As summarized in Figure 2, DV mice exhibited a significant augmentation in glycemia (2.5-fold), triglycerides (1.9-fold) and total cholesterol (2.3-fold) when compared with control ND mice (p < 0.05). The treatment of diabetic apoE−/− mice with quercetin caused significant attenuation of plasma glucose (~25%) and abolished the hypertriglyceridemia (p < 0.05); however, this dose of quercetin did not reverse the hypercholesterolemia.


The protective effects of oral low-dose quercetin on diabetic nephropathy in hypercholesterolemic mice.

Gomes IB, Porto ML, Santos MC, Campagnaro BP, Gava AL, Meyrelles SS, Pereira TM, Vasquez EC - Front Physiol (2015)

Total plasma glucose (A), triglycerides (B), and cholesterol (C) in diabetic apoE−/− mice treated with quercetin (DQ) compared with diabetic apoE−/− mice administered vehicle (DV) compared with non-diabetic apoE−/− (ND) mice. Values are the means ± SEM for n = 6–8 mice per group. *p < 0.05 vs. ND, #p < 0.05 vs. DV.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4557109&req=5

Figure 2: Total plasma glucose (A), triglycerides (B), and cholesterol (C) in diabetic apoE−/− mice treated with quercetin (DQ) compared with diabetic apoE−/− mice administered vehicle (DV) compared with non-diabetic apoE−/− (ND) mice. Values are the means ± SEM for n = 6–8 mice per group. *p < 0.05 vs. ND, #p < 0.05 vs. DV.
Mentions: As summarized in Figure 2, DV mice exhibited a significant augmentation in glycemia (2.5-fold), triglycerides (1.9-fold) and total cholesterol (2.3-fold) when compared with control ND mice (p < 0.05). The treatment of diabetic apoE−/− mice with quercetin caused significant attenuation of plasma glucose (~25%) and abolished the hypertriglyceridemia (p < 0.05); however, this dose of quercetin did not reverse the hypercholesterolemia.

Bottom Line: We also observed protective effects on the renal structural changes, including normalization of the index of glomerulosclerosis and kidney weight/body weight.Our data revealed that quercetin treatment significantly reduced DN in hypercholesterolemic mice by inducing biochemical changes (decrease in glucose and triglycerides serum levels) and reduction of glomerulosclerosis.Thus, this study highlights the relevance of quercetin as an alternative therapeutic option for DN, including in diabetes associated with dyslipidemia.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Translational Physiology, Health Sciences Center, Federal University of Espirito Santo Vitoria, Brazil.

ABSTRACT

Aims: Diabetic nephropathy (DN) is one of the most important causes of chronic renal disease, and the incidence of DN is increasing worldwide. Considering our previous report (Gomes et al., 2014) indicating that chronic treatment with oral low-dose quercetin (10 mg/Kg) demonstrated anti-oxidative, anti-apoptotic and renoprotective effects in the C57BL/6J model of DN, we investigated whether this flavonoid could also have beneficial effects in concurrent DN and spontaneous atherosclerosis using the apolipoprotein E-deficient mouse (apoE(-/-)).

Methods: Streptozotocin was used to induce diabetes (100 mg/kg/day, 3 days) in male apoE(-/-) mice (8 week-old). After 6 weeks, the mice were randomly separated into DQ: diabetic apoE(-/-) mice treated with quercetin (10 mg/kg/day, 4 weeks, n = 8), DV: diabetic ApoE(-/-) mice treated with vehicle (n = 8) and ND: non-treated non-diabetic mice (n = 8).

Results: Quercetin treatment diminished polyuria (~30%; p < 0.05), glycemia (~25%, p < 0.05), normalized the hypertriglyceridemia. Moreover, this bioflavonoid diminished creatininemia (~30%, p < 0.01) and reduced proteinuria but not to normal levels. We also observed protective effects on the renal structural changes, including normalization of the index of glomerulosclerosis and kidney weight/body weight.

Conclusions: Our data revealed that quercetin treatment significantly reduced DN in hypercholesterolemic mice by inducing biochemical changes (decrease in glucose and triglycerides serum levels) and reduction of glomerulosclerosis. Thus, this study highlights the relevance of quercetin as an alternative therapeutic option for DN, including in diabetes associated with dyslipidemia.

No MeSH data available.


Related in: MedlinePlus