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Chitinolytic Bacteria-Assisted Conversion of Squid Pen and Its Effect on Dyes and Pigments Adsorption.

Liang TW, Lo BC, Wang SL - Mar Drugs (2015)

Bottom Line: One chitosanase induced from squid pen powder (SPP)-containing medium by Bacillus cereus TKU034 was purified in high purification fold (441) and high yield of activity recovery (51%) by ammonium sulfate precipitation and combined column chromatography.The SDS-PAGE results showed its molecular mass to be around 43 kDa.The enzyme products revealed that the chitosanase could degrade chitosan with various degrees of polymerization, ranging from 3 to 9, as well as the chitosanase in an endolytic manner.

View Article: PubMed Central - PubMed

Affiliation: Life Science Development Center, Tamkang University, No. 151, Yingchuan Rd., Tamsui, New Taipei City 25137, Taiwan. ltw27@ms55.hinet.net.

ABSTRACT
The aim of this work was to produce chitosanase by fermenting from squid pen, and recover the fermented squid pen for dye removal by adsorption. One chitosanase induced from squid pen powder (SPP)-containing medium by Bacillus cereus TKU034 was purified in high purification fold (441) and high yield of activity recovery (51%) by ammonium sulfate precipitation and combined column chromatography. The SDS-PAGE results showed its molecular mass to be around 43 kDa. The TKU034 chitosanase used for the chitooligomers preparation was studied. The enzyme products revealed that the chitosanase could degrade chitosan with various degrees of polymerization, ranging from 3 to 9, as well as the chitosanase in an endolytic manner. Besides, the fermented SPP was recovered and displayed a better adsorption rate (up to 99.5%) for the disperse dyes (red, yellow, blue, and black) than the water-soluble food colorants, Allura Red AC (R40) and Tartrazine (Y4). The adsorbed R40 on the unfermented SPP and the fermented SPP was eluted by distilled water and 1 M NaOH to confirm the dye adsorption mechanism. The fermented SPP had a slightly higher adsorption capacity than the unfermented, and elution of the dye from the fermented SPP was easier than from the unfermented. The main dye adsorption mechanism of fermented SPP was physical adsorption, while the adsorption mechanism of unfermented SPP was chemical adsorption.

No MeSH data available.


Effects of temperature and pH on the activity and stability of the chitosanase. (a) optimum temperature (●) and thermal stability (○); (b,c) optimum pH (b) and pH stability (c), (●, acetate buffer; ○, phosphate buffer; ▼, sodium bicarbonate buffer).
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marinedrugs-13-04576-f003: Effects of temperature and pH on the activity and stability of the chitosanase. (a) optimum temperature (●) and thermal stability (○); (b,c) optimum pH (b) and pH stability (c), (●, acetate buffer; ○, phosphate buffer; ▼, sodium bicarbonate buffer).

Mentions: The effect of temperature and pH on the chitosanase from B. cereus TKU034 was investigated. For the effect of temperature on activity, the purified chitosanase from B. cereus TKU034 was active in the range 30–65 °C, being most active at 50 °C. It retained 68% of its highest activity at 65 °C (Figure 3a).


Chitinolytic Bacteria-Assisted Conversion of Squid Pen and Its Effect on Dyes and Pigments Adsorption.

Liang TW, Lo BC, Wang SL - Mar Drugs (2015)

Effects of temperature and pH on the activity and stability of the chitosanase. (a) optimum temperature (●) and thermal stability (○); (b,c) optimum pH (b) and pH stability (c), (●, acetate buffer; ○, phosphate buffer; ▼, sodium bicarbonate buffer).
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4556994&req=5

marinedrugs-13-04576-f003: Effects of temperature and pH on the activity and stability of the chitosanase. (a) optimum temperature (●) and thermal stability (○); (b,c) optimum pH (b) and pH stability (c), (●, acetate buffer; ○, phosphate buffer; ▼, sodium bicarbonate buffer).
Mentions: The effect of temperature and pH on the chitosanase from B. cereus TKU034 was investigated. For the effect of temperature on activity, the purified chitosanase from B. cereus TKU034 was active in the range 30–65 °C, being most active at 50 °C. It retained 68% of its highest activity at 65 °C (Figure 3a).

Bottom Line: One chitosanase induced from squid pen powder (SPP)-containing medium by Bacillus cereus TKU034 was purified in high purification fold (441) and high yield of activity recovery (51%) by ammonium sulfate precipitation and combined column chromatography.The SDS-PAGE results showed its molecular mass to be around 43 kDa.The enzyme products revealed that the chitosanase could degrade chitosan with various degrees of polymerization, ranging from 3 to 9, as well as the chitosanase in an endolytic manner.

View Article: PubMed Central - PubMed

Affiliation: Life Science Development Center, Tamkang University, No. 151, Yingchuan Rd., Tamsui, New Taipei City 25137, Taiwan. ltw27@ms55.hinet.net.

ABSTRACT
The aim of this work was to produce chitosanase by fermenting from squid pen, and recover the fermented squid pen for dye removal by adsorption. One chitosanase induced from squid pen powder (SPP)-containing medium by Bacillus cereus TKU034 was purified in high purification fold (441) and high yield of activity recovery (51%) by ammonium sulfate precipitation and combined column chromatography. The SDS-PAGE results showed its molecular mass to be around 43 kDa. The TKU034 chitosanase used for the chitooligomers preparation was studied. The enzyme products revealed that the chitosanase could degrade chitosan with various degrees of polymerization, ranging from 3 to 9, as well as the chitosanase in an endolytic manner. Besides, the fermented SPP was recovered and displayed a better adsorption rate (up to 99.5%) for the disperse dyes (red, yellow, blue, and black) than the water-soluble food colorants, Allura Red AC (R40) and Tartrazine (Y4). The adsorbed R40 on the unfermented SPP and the fermented SPP was eluted by distilled water and 1 M NaOH to confirm the dye adsorption mechanism. The fermented SPP had a slightly higher adsorption capacity than the unfermented, and elution of the dye from the fermented SPP was easier than from the unfermented. The main dye adsorption mechanism of fermented SPP was physical adsorption, while the adsorption mechanism of unfermented SPP was chemical adsorption.

No MeSH data available.