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Chitinolytic Bacteria-Assisted Conversion of Squid Pen and Its Effect on Dyes and Pigments Adsorption.

Liang TW, Lo BC, Wang SL - Mar Drugs (2015)

Bottom Line: One chitosanase induced from squid pen powder (SPP)-containing medium by Bacillus cereus TKU034 was purified in high purification fold (441) and high yield of activity recovery (51%) by ammonium sulfate precipitation and combined column chromatography.The SDS-PAGE results showed its molecular mass to be around 43 kDa.The enzyme products revealed that the chitosanase could degrade chitosan with various degrees of polymerization, ranging from 3 to 9, as well as the chitosanase in an endolytic manner.

View Article: PubMed Central - PubMed

Affiliation: Life Science Development Center, Tamkang University, No. 151, Yingchuan Rd., Tamsui, New Taipei City 25137, Taiwan. ltw27@ms55.hinet.net.

ABSTRACT
The aim of this work was to produce chitosanase by fermenting from squid pen, and recover the fermented squid pen for dye removal by adsorption. One chitosanase induced from squid pen powder (SPP)-containing medium by Bacillus cereus TKU034 was purified in high purification fold (441) and high yield of activity recovery (51%) by ammonium sulfate precipitation and combined column chromatography. The SDS-PAGE results showed its molecular mass to be around 43 kDa. The TKU034 chitosanase used for the chitooligomers preparation was studied. The enzyme products revealed that the chitosanase could degrade chitosan with various degrees of polymerization, ranging from 3 to 9, as well as the chitosanase in an endolytic manner. Besides, the fermented SPP was recovered and displayed a better adsorption rate (up to 99.5%) for the disperse dyes (red, yellow, blue, and black) than the water-soluble food colorants, Allura Red AC (R40) and Tartrazine (Y4). The adsorbed R40 on the unfermented SPP and the fermented SPP was eluted by distilled water and 1 M NaOH to confirm the dye adsorption mechanism. The fermented SPP had a slightly higher adsorption capacity than the unfermented, and elution of the dye from the fermented SPP was easier than from the unfermented. The main dye adsorption mechanism of fermented SPP was physical adsorption, while the adsorption mechanism of unfermented SPP was chemical adsorption.

No MeSH data available.


Time courses of chitosanase production from B.cereus TKU034 in squid pen containing media (●) and nutrient broth (NB) (△).
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marinedrugs-13-04576-f001: Time courses of chitosanase production from B.cereus TKU034 in squid pen containing media (●) and nutrient broth (NB) (△).

Mentions: As shown in Figure 1, the production of chitosanase by strain TKU034 was investigated over five days of cultivation in the production medium. The 100 mL of basal medium (0.1% K2HPO4 and 0.05% MgSO4·7H2O, pH 7) containing 1% SPP was the most suitable medium for the production of chitosanase by strain TKU034 at 37 °C. Compared with nutrient broth (NB), the SPP medium increased the enzyme production at the fourth day (Figure 1). The culture supernatant obtained from the bacterial culture in the presence of water-soluble chitosan as substrate displayed gradual chitosan degrading activity that dramatically showed increasing by-products up to four days of cultivation. Exponential growth of B. cereus TKU034 was observed for three days, and the stationary phase was reached at the fourth day. The highest chitosanase activity of B. cereus TKU034 was detected in the culture on the fourth day of bacterial growth (Figure 1). It was observed that the culture supernatant exerted strong chitosan degrading activities. Conclusively, the results suggested that the chitosanase from B. cereus TKU034 may be secreted extracellularly.


Chitinolytic Bacteria-Assisted Conversion of Squid Pen and Its Effect on Dyes and Pigments Adsorption.

Liang TW, Lo BC, Wang SL - Mar Drugs (2015)

Time courses of chitosanase production from B.cereus TKU034 in squid pen containing media (●) and nutrient broth (NB) (△).
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4556994&req=5

marinedrugs-13-04576-f001: Time courses of chitosanase production from B.cereus TKU034 in squid pen containing media (●) and nutrient broth (NB) (△).
Mentions: As shown in Figure 1, the production of chitosanase by strain TKU034 was investigated over five days of cultivation in the production medium. The 100 mL of basal medium (0.1% K2HPO4 and 0.05% MgSO4·7H2O, pH 7) containing 1% SPP was the most suitable medium for the production of chitosanase by strain TKU034 at 37 °C. Compared with nutrient broth (NB), the SPP medium increased the enzyme production at the fourth day (Figure 1). The culture supernatant obtained from the bacterial culture in the presence of water-soluble chitosan as substrate displayed gradual chitosan degrading activity that dramatically showed increasing by-products up to four days of cultivation. Exponential growth of B. cereus TKU034 was observed for three days, and the stationary phase was reached at the fourth day. The highest chitosanase activity of B. cereus TKU034 was detected in the culture on the fourth day of bacterial growth (Figure 1). It was observed that the culture supernatant exerted strong chitosan degrading activities. Conclusively, the results suggested that the chitosanase from B. cereus TKU034 may be secreted extracellularly.

Bottom Line: One chitosanase induced from squid pen powder (SPP)-containing medium by Bacillus cereus TKU034 was purified in high purification fold (441) and high yield of activity recovery (51%) by ammonium sulfate precipitation and combined column chromatography.The SDS-PAGE results showed its molecular mass to be around 43 kDa.The enzyme products revealed that the chitosanase could degrade chitosan with various degrees of polymerization, ranging from 3 to 9, as well as the chitosanase in an endolytic manner.

View Article: PubMed Central - PubMed

Affiliation: Life Science Development Center, Tamkang University, No. 151, Yingchuan Rd., Tamsui, New Taipei City 25137, Taiwan. ltw27@ms55.hinet.net.

ABSTRACT
The aim of this work was to produce chitosanase by fermenting from squid pen, and recover the fermented squid pen for dye removal by adsorption. One chitosanase induced from squid pen powder (SPP)-containing medium by Bacillus cereus TKU034 was purified in high purification fold (441) and high yield of activity recovery (51%) by ammonium sulfate precipitation and combined column chromatography. The SDS-PAGE results showed its molecular mass to be around 43 kDa. The TKU034 chitosanase used for the chitooligomers preparation was studied. The enzyme products revealed that the chitosanase could degrade chitosan with various degrees of polymerization, ranging from 3 to 9, as well as the chitosanase in an endolytic manner. Besides, the fermented SPP was recovered and displayed a better adsorption rate (up to 99.5%) for the disperse dyes (red, yellow, blue, and black) than the water-soluble food colorants, Allura Red AC (R40) and Tartrazine (Y4). The adsorbed R40 on the unfermented SPP and the fermented SPP was eluted by distilled water and 1 M NaOH to confirm the dye adsorption mechanism. The fermented SPP had a slightly higher adsorption capacity than the unfermented, and elution of the dye from the fermented SPP was easier than from the unfermented. The main dye adsorption mechanism of fermented SPP was physical adsorption, while the adsorption mechanism of unfermented SPP was chemical adsorption.

No MeSH data available.