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A study of chlorinated solvent contamination of the aquifers of an industrial area in central Italy: a possibility of bioremediation.

Matteucci F, Ercole C, Del Gallo M - Front Microbiol (2015)

Bottom Line: In Italy, there are many situations of serious contamination of groundwater that might compromise their use in industry, agriculture, private, as the critical case of a Central Italy valley located in the province of Teramo ("Val Vibrata"), characterized by a significant chlorinated solvents contamination.Data from the various monitoring campaigns that have taken place over time were collected, and new samplings were carried out, resulting in a complete database.Among the tested electron donors (i.e., yeast extract, lactate, and butyrate) lactate and butyrate enhanced dechlorination of chlorinated compounds.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Environmental Microbiology, Department of Life, Health and Environmental Sciences, University of L'Aquila L'Aquila, Italy.

ABSTRACT
Perchloroethene, trichloroethene, and other chlorinated solvents are widespread groundwater pollutants. They form dense non-aqueous phase liquids that sink through permeable groundwater aquifers until non-permeable zone is reached. In Italy, there are many situations of serious contamination of groundwater that might compromise their use in industry, agriculture, private, as the critical case of a Central Italy valley located in the province of Teramo ("Val Vibrata"), characterized by a significant chlorinated solvents contamination. Data from the various monitoring campaigns that have taken place over time were collected, and new samplings were carried out, resulting in a complete database. The data matrix was processed with a multivariate statistic analysis (in particular principal component analysis, PCA) and was then imported into geographic information system (GIS), to obtain a model of the contamination. A microcosm anaerobic study was utilized to assess the potential for in situ natural or enhanced bioremediation. Most of the microcosms were positive for dechlorination, particularly those inoculated with a mineral medium. This indicate the presence of an active native dechlorinating population in the subsurface, probably inhibited by co-contaminants in the groundwater, or more likely by the absence or lack of nutritional factors. Among the tested electron donors (i.e., yeast extract, lactate, and butyrate) lactate and butyrate enhanced dechlorination of chlorinated compounds. PCA and GIS studies allowed delimiting the contamination; the microcosm study helped to identify the conditions to promote the bioremediation of the area.

No MeSH data available.


Related in: MedlinePlus

(A) Time course of reductive dechlorination (RD) of PCE in microcosms 9 amended with lactate and PCE, MM, and yeast extract (YE). (B) Time course of RD of PCE in microcosms 12 amended with lactate and digester sludge, PCE, MM, and YE.
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Figure 4: (A) Time course of reductive dechlorination (RD) of PCE in microcosms 9 amended with lactate and PCE, MM, and yeast extract (YE). (B) Time course of RD of PCE in microcosms 12 amended with lactate and digester sludge, PCE, MM, and YE.

Mentions: In lactate-amended microcosms a complete degradation of PCE and TCE with formation of ethene occurs. Figure 4A shows the trend of microcosm 9, set up with soil and water from S3 drilling and amended with lactate. The dechlorinating activity began after a lag period of about 60 days, and led to a rapid accumulation of TCE, with the subsequent formation, by hydrogenolysis, of cis-DCE. The microcosm 3, amended with lactate, but set up with soil and water from S2 drilling, has the same trend (results not shown). Figure 4B shows the trend of microcosm 12, set up with soil and water from S3 drilling, with the addition of anaerobic sludge and amended with lactate. In this microcosm dechlorinating activity began after a lag period of about 20 days, and led to a rapid accumulation of TCE and subsequently of cis-DCE. DCE was gradually transformed into VC; ethene formation from VC was a slow process. However, at the end of the incubation all VC was converted to ethene.


A study of chlorinated solvent contamination of the aquifers of an industrial area in central Italy: a possibility of bioremediation.

Matteucci F, Ercole C, Del Gallo M - Front Microbiol (2015)

(A) Time course of reductive dechlorination (RD) of PCE in microcosms 9 amended with lactate and PCE, MM, and yeast extract (YE). (B) Time course of RD of PCE in microcosms 12 amended with lactate and digester sludge, PCE, MM, and YE.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4556989&req=5

Figure 4: (A) Time course of reductive dechlorination (RD) of PCE in microcosms 9 amended with lactate and PCE, MM, and yeast extract (YE). (B) Time course of RD of PCE in microcosms 12 amended with lactate and digester sludge, PCE, MM, and YE.
Mentions: In lactate-amended microcosms a complete degradation of PCE and TCE with formation of ethene occurs. Figure 4A shows the trend of microcosm 9, set up with soil and water from S3 drilling and amended with lactate. The dechlorinating activity began after a lag period of about 60 days, and led to a rapid accumulation of TCE, with the subsequent formation, by hydrogenolysis, of cis-DCE. The microcosm 3, amended with lactate, but set up with soil and water from S2 drilling, has the same trend (results not shown). Figure 4B shows the trend of microcosm 12, set up with soil and water from S3 drilling, with the addition of anaerobic sludge and amended with lactate. In this microcosm dechlorinating activity began after a lag period of about 20 days, and led to a rapid accumulation of TCE and subsequently of cis-DCE. DCE was gradually transformed into VC; ethene formation from VC was a slow process. However, at the end of the incubation all VC was converted to ethene.

Bottom Line: In Italy, there are many situations of serious contamination of groundwater that might compromise their use in industry, agriculture, private, as the critical case of a Central Italy valley located in the province of Teramo ("Val Vibrata"), characterized by a significant chlorinated solvents contamination.Data from the various monitoring campaigns that have taken place over time were collected, and new samplings were carried out, resulting in a complete database.Among the tested electron donors (i.e., yeast extract, lactate, and butyrate) lactate and butyrate enhanced dechlorination of chlorinated compounds.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Environmental Microbiology, Department of Life, Health and Environmental Sciences, University of L'Aquila L'Aquila, Italy.

ABSTRACT
Perchloroethene, trichloroethene, and other chlorinated solvents are widespread groundwater pollutants. They form dense non-aqueous phase liquids that sink through permeable groundwater aquifers until non-permeable zone is reached. In Italy, there are many situations of serious contamination of groundwater that might compromise their use in industry, agriculture, private, as the critical case of a Central Italy valley located in the province of Teramo ("Val Vibrata"), characterized by a significant chlorinated solvents contamination. Data from the various monitoring campaigns that have taken place over time were collected, and new samplings were carried out, resulting in a complete database. The data matrix was processed with a multivariate statistic analysis (in particular principal component analysis, PCA) and was then imported into geographic information system (GIS), to obtain a model of the contamination. A microcosm anaerobic study was utilized to assess the potential for in situ natural or enhanced bioremediation. Most of the microcosms were positive for dechlorination, particularly those inoculated with a mineral medium. This indicate the presence of an active native dechlorinating population in the subsurface, probably inhibited by co-contaminants in the groundwater, or more likely by the absence or lack of nutritional factors. Among the tested electron donors (i.e., yeast extract, lactate, and butyrate) lactate and butyrate enhanced dechlorination of chlorinated compounds. PCA and GIS studies allowed delimiting the contamination; the microcosm study helped to identify the conditions to promote the bioremediation of the area.

No MeSH data available.


Related in: MedlinePlus