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Characteristics of Tumor Infiltrating Lymphocyte and Circulating Lymphocyte Repertoires in Pancreatic Cancer by the Sequencing of T Cell Receptors.

Bai X, Zhang Q, Wu S, Zhang X, Wang M, He F, Wei T, Yang J, Lou Y, Cai Z, Liang T - Sci Rep (2015)

Bottom Line: By analyzing the complementary determining region 3 (CDR3) gene sequence, we found no significant differences in the T cell receptor (TCR) repertoires between patients and healthy controls.In addition, clonotypes with low frequencies were found in significantly higher numbers in primary pancreatic tumors compared to blood samples from patients and healthy controls.The results imply that specific types of pancreatic cancer share potentially important immunological characteristics.

View Article: PubMed Central - PubMed

Affiliation: Department of Hepatobiliary and Pancreatic Surgery, the Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, China.

ABSTRACT
Pancreatic cancer has a poor prognosis and few effective treatments. The failure of treatment is partially due to the high heterogeneity of cancer cells within the tumor. T cells target and kill cancer cells by the specific recognition of cancer-associated antigens. In this study, T cells from primary tumor and blood of sixteen patients with pancreatic cancer were characterized by deep sequencing. T cells from blood of another eight healthy volunteers were also studied as controls. By analyzing the complementary determining region 3 (CDR3) gene sequence, we found no significant differences in the T cell receptor (TCR) repertoires between patients and healthy controls. Types and length of CDR3 were similar among groups. However, two clusters of patients were identified according to the degree of CDR3 overlap within tumor sample group. In addition, clonotypes with low frequencies were found in significantly higher numbers in primary pancreatic tumors compared to blood samples from patients and healthy controls. This study is the first to characterize the TCR repertoires of pancreatic cancers in both primary tumors and matched blood samples. The results imply that specific types of pancreatic cancer share potentially important immunological characteristics.

No MeSH data available.


Related in: MedlinePlus

Differences in different clonotypes in the three groups.T cell clonotypes were categorized by different frequencies. (a) Tumor tissue samples showed significantly lower levels of medium frequency (0.001%–0.01%) clonotype compared to blood samples from the patients and healthy controls. (b) Tumor tissue samples showed significantly lower levels of medium frequency (<0.001%) clonotype compared to blood samples from the patients and healthy controls. ****P < 0.0001, *****P < 10−5, **********P < 10−10.
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f7: Differences in different clonotypes in the three groups.T cell clonotypes were categorized by different frequencies. (a) Tumor tissue samples showed significantly lower levels of medium frequency (0.001%–0.01%) clonotype compared to blood samples from the patients and healthy controls. (b) Tumor tissue samples showed significantly lower levels of medium frequency (<0.001%) clonotype compared to blood samples from the patients and healthy controls. ****P < 0.0001, *****P < 10−5, **********P < 10−10.

Mentions: CDR3 clonotypes were further classified into five groups according to their abundance, and divided by 0.001%, 0.01%, or 1% of 106 analyzed T cells (Table 1). The small and medium groups occupied between 80% and 90% of clonotypes in the PCT samples. The hyper-expanded and large groups of clonotypes showed insignificant differences between groups (Fig. S4). However, there was a lower proportion of the medium clonotypes and a higher proportion of the small clonotypes in the PCT group compared to those in the PCB and HVB groups (Fig. 7).


Characteristics of Tumor Infiltrating Lymphocyte and Circulating Lymphocyte Repertoires in Pancreatic Cancer by the Sequencing of T Cell Receptors.

Bai X, Zhang Q, Wu S, Zhang X, Wang M, He F, Wei T, Yang J, Lou Y, Cai Z, Liang T - Sci Rep (2015)

Differences in different clonotypes in the three groups.T cell clonotypes were categorized by different frequencies. (a) Tumor tissue samples showed significantly lower levels of medium frequency (0.001%–0.01%) clonotype compared to blood samples from the patients and healthy controls. (b) Tumor tissue samples showed significantly lower levels of medium frequency (<0.001%) clonotype compared to blood samples from the patients and healthy controls. ****P < 0.0001, *****P < 10−5, **********P < 10−10.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4556988&req=5

f7: Differences in different clonotypes in the three groups.T cell clonotypes were categorized by different frequencies. (a) Tumor tissue samples showed significantly lower levels of medium frequency (0.001%–0.01%) clonotype compared to blood samples from the patients and healthy controls. (b) Tumor tissue samples showed significantly lower levels of medium frequency (<0.001%) clonotype compared to blood samples from the patients and healthy controls. ****P < 0.0001, *****P < 10−5, **********P < 10−10.
Mentions: CDR3 clonotypes were further classified into five groups according to their abundance, and divided by 0.001%, 0.01%, or 1% of 106 analyzed T cells (Table 1). The small and medium groups occupied between 80% and 90% of clonotypes in the PCT samples. The hyper-expanded and large groups of clonotypes showed insignificant differences between groups (Fig. S4). However, there was a lower proportion of the medium clonotypes and a higher proportion of the small clonotypes in the PCT group compared to those in the PCB and HVB groups (Fig. 7).

Bottom Line: By analyzing the complementary determining region 3 (CDR3) gene sequence, we found no significant differences in the T cell receptor (TCR) repertoires between patients and healthy controls.In addition, clonotypes with low frequencies were found in significantly higher numbers in primary pancreatic tumors compared to blood samples from patients and healthy controls.The results imply that specific types of pancreatic cancer share potentially important immunological characteristics.

View Article: PubMed Central - PubMed

Affiliation: Department of Hepatobiliary and Pancreatic Surgery, the Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, China.

ABSTRACT
Pancreatic cancer has a poor prognosis and few effective treatments. The failure of treatment is partially due to the high heterogeneity of cancer cells within the tumor. T cells target and kill cancer cells by the specific recognition of cancer-associated antigens. In this study, T cells from primary tumor and blood of sixteen patients with pancreatic cancer were characterized by deep sequencing. T cells from blood of another eight healthy volunteers were also studied as controls. By analyzing the complementary determining region 3 (CDR3) gene sequence, we found no significant differences in the T cell receptor (TCR) repertoires between patients and healthy controls. Types and length of CDR3 were similar among groups. However, two clusters of patients were identified according to the degree of CDR3 overlap within tumor sample group. In addition, clonotypes with low frequencies were found in significantly higher numbers in primary pancreatic tumors compared to blood samples from patients and healthy controls. This study is the first to characterize the TCR repertoires of pancreatic cancers in both primary tumors and matched blood samples. The results imply that specific types of pancreatic cancer share potentially important immunological characteristics.

No MeSH data available.


Related in: MedlinePlus