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Characteristics of Tumor Infiltrating Lymphocyte and Circulating Lymphocyte Repertoires in Pancreatic Cancer by the Sequencing of T Cell Receptors.

Bai X, Zhang Q, Wu S, Zhang X, Wang M, He F, Wei T, Yang J, Lou Y, Cai Z, Liang T - Sci Rep (2015)

Bottom Line: By analyzing the complementary determining region 3 (CDR3) gene sequence, we found no significant differences in the T cell receptor (TCR) repertoires between patients and healthy controls.In addition, clonotypes with low frequencies were found in significantly higher numbers in primary pancreatic tumors compared to blood samples from patients and healthy controls.The results imply that specific types of pancreatic cancer share potentially important immunological characteristics.

View Article: PubMed Central - PubMed

Affiliation: Department of Hepatobiliary and Pancreatic Surgery, the Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, China.

ABSTRACT
Pancreatic cancer has a poor prognosis and few effective treatments. The failure of treatment is partially due to the high heterogeneity of cancer cells within the tumor. T cells target and kill cancer cells by the specific recognition of cancer-associated antigens. In this study, T cells from primary tumor and blood of sixteen patients with pancreatic cancer were characterized by deep sequencing. T cells from blood of another eight healthy volunteers were also studied as controls. By analyzing the complementary determining region 3 (CDR3) gene sequence, we found no significant differences in the T cell receptor (TCR) repertoires between patients and healthy controls. Types and length of CDR3 were similar among groups. However, two clusters of patients were identified according to the degree of CDR3 overlap within tumor sample group. In addition, clonotypes with low frequencies were found in significantly higher numbers in primary pancreatic tumors compared to blood samples from patients and healthy controls. This study is the first to characterize the TCR repertoires of pancreatic cancers in both primary tumors and matched blood samples. The results imply that specific types of pancreatic cancer share potentially important immunological characteristics.

No MeSH data available.


Related in: MedlinePlus

Diversity of TCR repertoires was similar among groups.Inverse Simpson’s diversity index was used to assess the diversity of TCR repertoires, and no significant differences were found among these groups.
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f4: Diversity of TCR repertoires was similar among groups.Inverse Simpson’s diversity index was used to assess the diversity of TCR repertoires, and no significant differences were found among these groups.

Mentions: We used the inverse Simpson’s index to measure the diversity levels of the TCR repertoire in different groups, and no significant differences were found among the groups (Fig. 4). Moreover, we assessed the diversity of CDR3 by analyzing the transcript length distribution. The length of CDR3 was mainly between 39–45 base pairs (bp), and there was no significant difference with regards to the length distribution between groups (Fig. 5).


Characteristics of Tumor Infiltrating Lymphocyte and Circulating Lymphocyte Repertoires in Pancreatic Cancer by the Sequencing of T Cell Receptors.

Bai X, Zhang Q, Wu S, Zhang X, Wang M, He F, Wei T, Yang J, Lou Y, Cai Z, Liang T - Sci Rep (2015)

Diversity of TCR repertoires was similar among groups.Inverse Simpson’s diversity index was used to assess the diversity of TCR repertoires, and no significant differences were found among these groups.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4556988&req=5

f4: Diversity of TCR repertoires was similar among groups.Inverse Simpson’s diversity index was used to assess the diversity of TCR repertoires, and no significant differences were found among these groups.
Mentions: We used the inverse Simpson’s index to measure the diversity levels of the TCR repertoire in different groups, and no significant differences were found among the groups (Fig. 4). Moreover, we assessed the diversity of CDR3 by analyzing the transcript length distribution. The length of CDR3 was mainly between 39–45 base pairs (bp), and there was no significant difference with regards to the length distribution between groups (Fig. 5).

Bottom Line: By analyzing the complementary determining region 3 (CDR3) gene sequence, we found no significant differences in the T cell receptor (TCR) repertoires between patients and healthy controls.In addition, clonotypes with low frequencies were found in significantly higher numbers in primary pancreatic tumors compared to blood samples from patients and healthy controls.The results imply that specific types of pancreatic cancer share potentially important immunological characteristics.

View Article: PubMed Central - PubMed

Affiliation: Department of Hepatobiliary and Pancreatic Surgery, the Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, China.

ABSTRACT
Pancreatic cancer has a poor prognosis and few effective treatments. The failure of treatment is partially due to the high heterogeneity of cancer cells within the tumor. T cells target and kill cancer cells by the specific recognition of cancer-associated antigens. In this study, T cells from primary tumor and blood of sixteen patients with pancreatic cancer were characterized by deep sequencing. T cells from blood of another eight healthy volunteers were also studied as controls. By analyzing the complementary determining region 3 (CDR3) gene sequence, we found no significant differences in the T cell receptor (TCR) repertoires between patients and healthy controls. Types and length of CDR3 were similar among groups. However, two clusters of patients were identified according to the degree of CDR3 overlap within tumor sample group. In addition, clonotypes with low frequencies were found in significantly higher numbers in primary pancreatic tumors compared to blood samples from patients and healthy controls. This study is the first to characterize the TCR repertoires of pancreatic cancers in both primary tumors and matched blood samples. The results imply that specific types of pancreatic cancer share potentially important immunological characteristics.

No MeSH data available.


Related in: MedlinePlus