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Characteristics of Tumor Infiltrating Lymphocyte and Circulating Lymphocyte Repertoires in Pancreatic Cancer by the Sequencing of T Cell Receptors.

Bai X, Zhang Q, Wu S, Zhang X, Wang M, He F, Wei T, Yang J, Lou Y, Cai Z, Liang T - Sci Rep (2015)

Bottom Line: By analyzing the complementary determining region 3 (CDR3) gene sequence, we found no significant differences in the T cell receptor (TCR) repertoires between patients and healthy controls.In addition, clonotypes with low frequencies were found in significantly higher numbers in primary pancreatic tumors compared to blood samples from patients and healthy controls.The results imply that specific types of pancreatic cancer share potentially important immunological characteristics.

View Article: PubMed Central - PubMed

Affiliation: Department of Hepatobiliary and Pancreatic Surgery, the Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, China.

ABSTRACT
Pancreatic cancer has a poor prognosis and few effective treatments. The failure of treatment is partially due to the high heterogeneity of cancer cells within the tumor. T cells target and kill cancer cells by the specific recognition of cancer-associated antigens. In this study, T cells from primary tumor and blood of sixteen patients with pancreatic cancer were characterized by deep sequencing. T cells from blood of another eight healthy volunteers were also studied as controls. By analyzing the complementary determining region 3 (CDR3) gene sequence, we found no significant differences in the T cell receptor (TCR) repertoires between patients and healthy controls. Types and length of CDR3 were similar among groups. However, two clusters of patients were identified according to the degree of CDR3 overlap within tumor sample group. In addition, clonotypes with low frequencies were found in significantly higher numbers in primary pancreatic tumors compared to blood samples from patients and healthy controls. This study is the first to characterize the TCR repertoires of pancreatic cancers in both primary tumors and matched blood samples. The results imply that specific types of pancreatic cancer share potentially important immunological characteristics.

No MeSH data available.


Related in: MedlinePlus

The top-20 expressed gene of the V gene segments in each group.No significant differences were detected between groups. The TRBV20-1 and TRBV2 variants were the most frequent in most samples.
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f2: The top-20 expressed gene of the V gene segments in each group.No significant differences were detected between groups. The TRBV20-1 and TRBV2 variants were the most frequent in most samples.

Mentions: We analyzed the highly expressed V and J gene segments, as well as the V-J gene pair, in all three groups, respectively. The top-20 genes expressed in each sample are listed in Fig. 2. Similar patterns were found in all these samples, and no significant difference was detected among the three groups. In particular, for the V gene, the TRBV20-1 and TRBV2 variants were commonly overexpressed in all samples (Fig. 2), while for the J gene, the TRBJ2-7 and TRBJ2-1 were the most abundant variants (Fig. S1). In addition, the TRBV20-1;TRBJ2-7 and TRBV20-1;TRBJ2-1 were the most highly expressed gene pairs in these samples.


Characteristics of Tumor Infiltrating Lymphocyte and Circulating Lymphocyte Repertoires in Pancreatic Cancer by the Sequencing of T Cell Receptors.

Bai X, Zhang Q, Wu S, Zhang X, Wang M, He F, Wei T, Yang J, Lou Y, Cai Z, Liang T - Sci Rep (2015)

The top-20 expressed gene of the V gene segments in each group.No significant differences were detected between groups. The TRBV20-1 and TRBV2 variants were the most frequent in most samples.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4556988&req=5

f2: The top-20 expressed gene of the V gene segments in each group.No significant differences were detected between groups. The TRBV20-1 and TRBV2 variants were the most frequent in most samples.
Mentions: We analyzed the highly expressed V and J gene segments, as well as the V-J gene pair, in all three groups, respectively. The top-20 genes expressed in each sample are listed in Fig. 2. Similar patterns were found in all these samples, and no significant difference was detected among the three groups. In particular, for the V gene, the TRBV20-1 and TRBV2 variants were commonly overexpressed in all samples (Fig. 2), while for the J gene, the TRBJ2-7 and TRBJ2-1 were the most abundant variants (Fig. S1). In addition, the TRBV20-1;TRBJ2-7 and TRBV20-1;TRBJ2-1 were the most highly expressed gene pairs in these samples.

Bottom Line: By analyzing the complementary determining region 3 (CDR3) gene sequence, we found no significant differences in the T cell receptor (TCR) repertoires between patients and healthy controls.In addition, clonotypes with low frequencies were found in significantly higher numbers in primary pancreatic tumors compared to blood samples from patients and healthy controls.The results imply that specific types of pancreatic cancer share potentially important immunological characteristics.

View Article: PubMed Central - PubMed

Affiliation: Department of Hepatobiliary and Pancreatic Surgery, the Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, China.

ABSTRACT
Pancreatic cancer has a poor prognosis and few effective treatments. The failure of treatment is partially due to the high heterogeneity of cancer cells within the tumor. T cells target and kill cancer cells by the specific recognition of cancer-associated antigens. In this study, T cells from primary tumor and blood of sixteen patients with pancreatic cancer were characterized by deep sequencing. T cells from blood of another eight healthy volunteers were also studied as controls. By analyzing the complementary determining region 3 (CDR3) gene sequence, we found no significant differences in the T cell receptor (TCR) repertoires between patients and healthy controls. Types and length of CDR3 were similar among groups. However, two clusters of patients were identified according to the degree of CDR3 overlap within tumor sample group. In addition, clonotypes with low frequencies were found in significantly higher numbers in primary pancreatic tumors compared to blood samples from patients and healthy controls. This study is the first to characterize the TCR repertoires of pancreatic cancers in both primary tumors and matched blood samples. The results imply that specific types of pancreatic cancer share potentially important immunological characteristics.

No MeSH data available.


Related in: MedlinePlus