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Frankincense and myrrh suppress inflammation via regulation of the metabolic profiling and the MAPK signaling pathway.

Su S, Duan J, Chen T, Huang X, Shang E, Yu L, Wei K, Zhu Y, Guo J, Guo S, Liu P, Qian D, Tang Y - Sci Rep (2015)

Bottom Line: The results stated the elevated expression levels of TNFα, PGE2, IL-2, NO, and MDA in serum and swelling paw of AIA rats were significantly decreased after treatment, which exerted more remarkable inhibitive effects of combined therapy.The metbolic profiling of plasma and urine were clearly improved and twenty-one potential biomarkers were identified.Moreover, the inhibited effects of five bioactive components on cytokine transcription in PHA stimulated-PBMC showed the MAPK pathway might account for this phenomenon with considerable reduction in phosphorylated forms of all the three MAPK (ERK1/2, p38 and JNK) and down regulation of c-jun and c-fos.

View Article: PubMed Central - PubMed

Affiliation: Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, National and Local Collaborative Engineering Center of Chinese Medicinal Resources Industrialization and Formulae Innovative Medicine; Nanjing University of Chinese Medicine, Nanjing 210023, PR China.

ABSTRACT
Frankincense and myrrh are highly effective in treatment of inflammatary diseases, but lacking of the therapy mechanisms. We undertook this stuty to evaluate the effects on Adjuvant-induced Arthritis (AIA) rats and to explore the underlying mechanisms by analyzing the metabolic profiling and signaling pathway evaluated by expression of inflammatory cytokines, c-jun and c-fos and corresponding phosphorylation levels. The results stated the elevated expression levels of TNFα, PGE2, IL-2, NO, and MDA in serum and swelling paw of AIA rats were significantly decreased after treatment, which exerted more remarkable inhibitive effects of combined therapy. The metbolic profiling of plasma and urine were clearly improved and twenty-one potential biomarkers were identified. Moreover, the inhibited effects of five bioactive components on cytokine transcription in PHA stimulated-PBMC showed the MAPK pathway might account for this phenomenon with considerable reduction in phosphorylated forms of all the three MAPK (ERK1/2, p38 and JNK) and down regulation of c-jun and c-fos.

No MeSH data available.


Related in: MedlinePlus

The chemical structures of five bioactive compounds drived from Frankincense (compounds 1, 4, and 5) and Myrrh (compounds 2 and 3) (1. 3-hydroxylanosta-8,24-dien-21-oic-acid; 2. 2-Methoxy-5-acetoxy-fruranogermacr -1(10)-en-6-one; 3. abietic acid; 4. elemonic acid; 5. Acetyl elemolic acid).
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f1: The chemical structures of five bioactive compounds drived from Frankincense (compounds 1, 4, and 5) and Myrrh (compounds 2 and 3) (1. 3-hydroxylanosta-8,24-dien-21-oic-acid; 2. 2-Methoxy-5-acetoxy-fruranogermacr -1(10)-en-6-one; 3. abietic acid; 4. elemonic acid; 5. Acetyl elemolic acid).

Mentions: So, in this study, the adjuvant-induced arthritis (AIA) as immune-mediated rat model was used to assess the anti-arthritic efficacy of individual and combined extracts of Frankincense and Myrrh resin. The levels of TNF-α, and IL-2, PGE2, NO, and MDA were determined. To elucidate the mechanism, the metabolic changes in plasma and urine from AIA rats based on UPLC/Q-TOFMS were investigated. The potential biomarkers and metabolic pathway were also identified. Furthermore, the actions of five bioactive compounds (chemical structures see Fig. 1) drived from Frankincense (compounds 1, 4, and 5) and Myrrh (compounds 2 and 3), respectively, were investigated on TNFα, IL-1β, IL-2, IL-10, IL-12, INFγ, and c-jun and c-fos expression in PHA stimulated-PBMC (peripheral blood mononuclear cell) to explore the possible signaling pathways. These data would be useful to further develop and improve the anti-inflammatory agents.


Frankincense and myrrh suppress inflammation via regulation of the metabolic profiling and the MAPK signaling pathway.

Su S, Duan J, Chen T, Huang X, Shang E, Yu L, Wei K, Zhu Y, Guo J, Guo S, Liu P, Qian D, Tang Y - Sci Rep (2015)

The chemical structures of five bioactive compounds drived from Frankincense (compounds 1, 4, and 5) and Myrrh (compounds 2 and 3) (1. 3-hydroxylanosta-8,24-dien-21-oic-acid; 2. 2-Methoxy-5-acetoxy-fruranogermacr -1(10)-en-6-one; 3. abietic acid; 4. elemonic acid; 5. Acetyl elemolic acid).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4556964&req=5

f1: The chemical structures of five bioactive compounds drived from Frankincense (compounds 1, 4, and 5) and Myrrh (compounds 2 and 3) (1. 3-hydroxylanosta-8,24-dien-21-oic-acid; 2. 2-Methoxy-5-acetoxy-fruranogermacr -1(10)-en-6-one; 3. abietic acid; 4. elemonic acid; 5. Acetyl elemolic acid).
Mentions: So, in this study, the adjuvant-induced arthritis (AIA) as immune-mediated rat model was used to assess the anti-arthritic efficacy of individual and combined extracts of Frankincense and Myrrh resin. The levels of TNF-α, and IL-2, PGE2, NO, and MDA were determined. To elucidate the mechanism, the metabolic changes in plasma and urine from AIA rats based on UPLC/Q-TOFMS were investigated. The potential biomarkers and metabolic pathway were also identified. Furthermore, the actions of five bioactive compounds (chemical structures see Fig. 1) drived from Frankincense (compounds 1, 4, and 5) and Myrrh (compounds 2 and 3), respectively, were investigated on TNFα, IL-1β, IL-2, IL-10, IL-12, INFγ, and c-jun and c-fos expression in PHA stimulated-PBMC (peripheral blood mononuclear cell) to explore the possible signaling pathways. These data would be useful to further develop and improve the anti-inflammatory agents.

Bottom Line: The results stated the elevated expression levels of TNFα, PGE2, IL-2, NO, and MDA in serum and swelling paw of AIA rats were significantly decreased after treatment, which exerted more remarkable inhibitive effects of combined therapy.The metbolic profiling of plasma and urine were clearly improved and twenty-one potential biomarkers were identified.Moreover, the inhibited effects of five bioactive components on cytokine transcription in PHA stimulated-PBMC showed the MAPK pathway might account for this phenomenon with considerable reduction in phosphorylated forms of all the three MAPK (ERK1/2, p38 and JNK) and down regulation of c-jun and c-fos.

View Article: PubMed Central - PubMed

Affiliation: Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, National and Local Collaborative Engineering Center of Chinese Medicinal Resources Industrialization and Formulae Innovative Medicine; Nanjing University of Chinese Medicine, Nanjing 210023, PR China.

ABSTRACT
Frankincense and myrrh are highly effective in treatment of inflammatary diseases, but lacking of the therapy mechanisms. We undertook this stuty to evaluate the effects on Adjuvant-induced Arthritis (AIA) rats and to explore the underlying mechanisms by analyzing the metabolic profiling and signaling pathway evaluated by expression of inflammatory cytokines, c-jun and c-fos and corresponding phosphorylation levels. The results stated the elevated expression levels of TNFα, PGE2, IL-2, NO, and MDA in serum and swelling paw of AIA rats were significantly decreased after treatment, which exerted more remarkable inhibitive effects of combined therapy. The metbolic profiling of plasma and urine were clearly improved and twenty-one potential biomarkers were identified. Moreover, the inhibited effects of five bioactive components on cytokine transcription in PHA stimulated-PBMC showed the MAPK pathway might account for this phenomenon with considerable reduction in phosphorylated forms of all the three MAPK (ERK1/2, p38 and JNK) and down regulation of c-jun and c-fos.

No MeSH data available.


Related in: MedlinePlus