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Antimicrobial Effects and Resistant Regulation of Magnolol and Honokiol on Methicillin-Resistant Staphylococcus aureus.

Kim SY, Kim J, Jeong SI, Jahng KY, Yu KY - Biomed Res Int (2015)

Bottom Line: It was determined that magnolol and honokiol had a synergistic effect with oxacillin against MRSA strain.We concluded that the antibacterial activity of magnolol against MRSA strain is more related to the mecI's pathway and components of the cell wall than mecR1.Therefore, the results obtained in this study suggest that the combination of magnolol and antibiotics could lead to the development of new combination antibiotics against MRSA infection.

View Article: PubMed Central - PubMed

Affiliation: Jeonju Biomaterials Institute, Jeonju, Jeonbuk 561-360, Republic of Korea ; Department of Life Science, Chonbuk National University, Jeonju, Jeonbuk 561-756, Republic of Korea.

ABSTRACT
The antimicrobial killing activity toward methicillin-resistant Staphylococcus aureus (MRSA) has been a serious emerging global issue. In a continuing search for compounds with antibacterial activity against several microorganisms including S. aureus and MRSA, an n-hexane extract of Magnolia officinalis was found to contain magnolol. This compound exhibited potent activity against S. aureus, standard methicillin-susceptible S. aureus (MSSA), and MRSA as well as clinical MRSA isolates. When combined with oxacillin, the antibacterial activities of magnolol and honokiol against the MRSA strain were increased compared to single treatment without antibiotics at 10 µg/mL and 25 µg/mL, respectively. These activities of magnolol and honokiol were dose dependent. Also, magnolol showed synergistic effects with oxacillin against 13 clinical isolates of MRSA. It was determined that magnolol and honokiol had a synergistic effect with oxacillin against MRSA strain. Furthermore, the magnolol inhibited the expression of the resistant genes, mecA, mecI, femA, and femB, in mRNA. We concluded that the antibacterial activity of magnolol against MRSA strain is more related to the mecI's pathway and components of the cell wall than mecR1. Therefore, the results obtained in this study suggest that the combination of magnolol and antibiotics could lead to the development of new combination antibiotics against MRSA infection.

No MeSH data available.


Related in: MedlinePlus

Comparison of the purified magnolol isolated from Magnolia officinalis with standard compounds by HPLC.
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fig1: Comparison of the purified magnolol isolated from Magnolia officinalis with standard compounds by HPLC.

Mentions: Magnolol was purified from the bark of M. officinalis as described previously [27]. In brief, the methanol extracts were concentrated and lyophilized with a rotary evaporator and partitioned between water and n-hexane. The n-hexane fraction was then applied to a silica gel column eluted with an n-hexane: acetone (8 : 11, v/v) mixture. The n-hexane layer was concentrated, and magnolol was directly obtained by prep-HPLC of n-hexane layer. The isolated magnolol was identified by thin-layer chromatograph and mixed melting point determination in comparison with authentic magnolol (Wako, Osaka, Japan). The purity (≥98%) of magnolol was determined by high-performance thin-layer chromatograph (Figure 1).


Antimicrobial Effects and Resistant Regulation of Magnolol and Honokiol on Methicillin-Resistant Staphylococcus aureus.

Kim SY, Kim J, Jeong SI, Jahng KY, Yu KY - Biomed Res Int (2015)

Comparison of the purified magnolol isolated from Magnolia officinalis with standard compounds by HPLC.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4556871&req=5

fig1: Comparison of the purified magnolol isolated from Magnolia officinalis with standard compounds by HPLC.
Mentions: Magnolol was purified from the bark of M. officinalis as described previously [27]. In brief, the methanol extracts were concentrated and lyophilized with a rotary evaporator and partitioned between water and n-hexane. The n-hexane fraction was then applied to a silica gel column eluted with an n-hexane: acetone (8 : 11, v/v) mixture. The n-hexane layer was concentrated, and magnolol was directly obtained by prep-HPLC of n-hexane layer. The isolated magnolol was identified by thin-layer chromatograph and mixed melting point determination in comparison with authentic magnolol (Wako, Osaka, Japan). The purity (≥98%) of magnolol was determined by high-performance thin-layer chromatograph (Figure 1).

Bottom Line: It was determined that magnolol and honokiol had a synergistic effect with oxacillin against MRSA strain.We concluded that the antibacterial activity of magnolol against MRSA strain is more related to the mecI's pathway and components of the cell wall than mecR1.Therefore, the results obtained in this study suggest that the combination of magnolol and antibiotics could lead to the development of new combination antibiotics against MRSA infection.

View Article: PubMed Central - PubMed

Affiliation: Jeonju Biomaterials Institute, Jeonju, Jeonbuk 561-360, Republic of Korea ; Department of Life Science, Chonbuk National University, Jeonju, Jeonbuk 561-756, Republic of Korea.

ABSTRACT
The antimicrobial killing activity toward methicillin-resistant Staphylococcus aureus (MRSA) has been a serious emerging global issue. In a continuing search for compounds with antibacterial activity against several microorganisms including S. aureus and MRSA, an n-hexane extract of Magnolia officinalis was found to contain magnolol. This compound exhibited potent activity against S. aureus, standard methicillin-susceptible S. aureus (MSSA), and MRSA as well as clinical MRSA isolates. When combined with oxacillin, the antibacterial activities of magnolol and honokiol against the MRSA strain were increased compared to single treatment without antibiotics at 10 µg/mL and 25 µg/mL, respectively. These activities of magnolol and honokiol were dose dependent. Also, magnolol showed synergistic effects with oxacillin against 13 clinical isolates of MRSA. It was determined that magnolol and honokiol had a synergistic effect with oxacillin against MRSA strain. Furthermore, the magnolol inhibited the expression of the resistant genes, mecA, mecI, femA, and femB, in mRNA. We concluded that the antibacterial activity of magnolol against MRSA strain is more related to the mecI's pathway and components of the cell wall than mecR1. Therefore, the results obtained in this study suggest that the combination of magnolol and antibiotics could lead to the development of new combination antibiotics against MRSA infection.

No MeSH data available.


Related in: MedlinePlus