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Decorin-Mediated Inhibition of Human Trophoblast Cells Proliferation, Migration, and Invasion and Promotion of Apoptosis In Vitro.

Zou Y, Yu X, Lu J, Jiang Z, Zuo Q, Fan M, Huang S, Sun L - Biomed Res Int (2015)

Bottom Line: Preeclampsia (PE) is a unique complication of pregnancy, the pathogenesis of which has been generally accepted to be associated with the dysfunctions of extravillous trophoblast (EVT) including proliferation, apoptosis, and migration and invasion.Also the modulations of decorin on trophoblast cells' metastasis and invasion functions were detected through regulating the matrix metalloproteinases (MMP2 and MMP9).Thus, we suggested that the contribution of decorin to the modulation of trophoblast cells might have implications for the pathogenesis of preeclampsia.

View Article: PubMed Central - PubMed

Affiliation: Department of Obstetrics and Gynecology, The First Affiliated Hospital of Nanjing Medical University, 300 Guangzhou Road, Nanjing 210029, China.

ABSTRACT
Preeclampsia (PE) is a unique complication of pregnancy, the pathogenesis of which has been generally accepted to be associated with the dysfunctions of extravillous trophoblast (EVT) including proliferation, apoptosis, and migration and invasion. Decorin (DCN) has been proved to be a decidua-derived TGF-binding proteoglycan, which negatively regulates proliferation, migration, and invasiveness of human extravillous trophoblast cells. In this study, we identified a higher expression level of decorin in severe PE placentas by both real-time reverse transcription-polymerase chain reaction (qRT-PCR) and immunohistochemistry (IHC). And an inhibitory effect of decorin on proliferation, migration, and invasion and an enhanced effect on apoptosis in trophoblast cells HTR-8/SVneo and JEG-3 were validated in vitro. Also the modulations of decorin on trophoblast cells' metastasis and invasion functions were detected through regulating the matrix metalloproteinases (MMP2 and MMP9). Thus, we suggested that the contribution of decorin to the modulation of trophoblast cells might have implications for the pathogenesis of preeclampsia.

No MeSH data available.


Related in: MedlinePlus

Effects of decorin on growth and proliferation of trophoblast cells. ((a) and (b)) The proliferation ability of HTR-8/SVneo and JEG-3 cells was inhibited in pEGFP-DCN group compared to control, as identified by MTT assays. ((c) and (d)) Colony-forming assay showed a decrease of cells proliferation in pEGFP-DCN group compared to empty vector both in HTR-8/SVneo and in JEG-3 cells. ((e) and (f)) Cell-cycle analysis was performed 48 h following the treatment of HTR-8/SVneo and JEG-3 cells with pEGFP-DCN or empty vector. The DNA content was quantified by flow cytometric analysis. Values are represented as mean ± SEM (∗∗P < 0.01).
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fig3: Effects of decorin on growth and proliferation of trophoblast cells. ((a) and (b)) The proliferation ability of HTR-8/SVneo and JEG-3 cells was inhibited in pEGFP-DCN group compared to control, as identified by MTT assays. ((c) and (d)) Colony-forming assay showed a decrease of cells proliferation in pEGFP-DCN group compared to empty vector both in HTR-8/SVneo and in JEG-3 cells. ((e) and (f)) Cell-cycle analysis was performed 48 h following the treatment of HTR-8/SVneo and JEG-3 cells with pEGFP-DCN or empty vector. The DNA content was quantified by flow cytometric analysis. Values are represented as mean ± SEM (∗∗P < 0.01).

Mentions: The significant increase of decorin expression in PE placenta samples prompted us to explore the possible biologic significance of decorin in the pathogenesis of PE. To determine whether decorin affects trophoblast cells growth, we conducted MTT assay to detect cell growth viability in pEGFP-DCN transfected HTR-8/SVneo and JEG-3 cells compared to that of control (Figures 3(a) and 3(b), P < 0.01). Also, the impact of decorin on cell proliferation was assessed by colony formation assay. According to the colony formation assay, we found that cells transiently transfected with pEGFP-DCN had significantly reduced proliferation of cells compared with that of cells transfected with pEGFP-N1 (Figures 3(c) and 3(d), P < 0.01). Additionally, flow cytometric analysis was used to further examine whether the inhibition of decorin on cell proliferation reflected cell-cycle arrest. The cell-cycle analysis showed that cells transfected with pEGFP-DCN had an obvious cell-cycle arrest at the G1-G0 phase with a decreased G2–S-phase compared to that of negative control (Figures 3(e) and 3(f), P < 0.01).


Decorin-Mediated Inhibition of Human Trophoblast Cells Proliferation, Migration, and Invasion and Promotion of Apoptosis In Vitro.

Zou Y, Yu X, Lu J, Jiang Z, Zuo Q, Fan M, Huang S, Sun L - Biomed Res Int (2015)

Effects of decorin on growth and proliferation of trophoblast cells. ((a) and (b)) The proliferation ability of HTR-8/SVneo and JEG-3 cells was inhibited in pEGFP-DCN group compared to control, as identified by MTT assays. ((c) and (d)) Colony-forming assay showed a decrease of cells proliferation in pEGFP-DCN group compared to empty vector both in HTR-8/SVneo and in JEG-3 cells. ((e) and (f)) Cell-cycle analysis was performed 48 h following the treatment of HTR-8/SVneo and JEG-3 cells with pEGFP-DCN or empty vector. The DNA content was quantified by flow cytometric analysis. Values are represented as mean ± SEM (∗∗P < 0.01).
© Copyright Policy
Related In: Results  -  Collection

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Show All Figures
getmorefigures.php?uid=PMC4556865&req=5

fig3: Effects of decorin on growth and proliferation of trophoblast cells. ((a) and (b)) The proliferation ability of HTR-8/SVneo and JEG-3 cells was inhibited in pEGFP-DCN group compared to control, as identified by MTT assays. ((c) and (d)) Colony-forming assay showed a decrease of cells proliferation in pEGFP-DCN group compared to empty vector both in HTR-8/SVneo and in JEG-3 cells. ((e) and (f)) Cell-cycle analysis was performed 48 h following the treatment of HTR-8/SVneo and JEG-3 cells with pEGFP-DCN or empty vector. The DNA content was quantified by flow cytometric analysis. Values are represented as mean ± SEM (∗∗P < 0.01).
Mentions: The significant increase of decorin expression in PE placenta samples prompted us to explore the possible biologic significance of decorin in the pathogenesis of PE. To determine whether decorin affects trophoblast cells growth, we conducted MTT assay to detect cell growth viability in pEGFP-DCN transfected HTR-8/SVneo and JEG-3 cells compared to that of control (Figures 3(a) and 3(b), P < 0.01). Also, the impact of decorin on cell proliferation was assessed by colony formation assay. According to the colony formation assay, we found that cells transiently transfected with pEGFP-DCN had significantly reduced proliferation of cells compared with that of cells transfected with pEGFP-N1 (Figures 3(c) and 3(d), P < 0.01). Additionally, flow cytometric analysis was used to further examine whether the inhibition of decorin on cell proliferation reflected cell-cycle arrest. The cell-cycle analysis showed that cells transfected with pEGFP-DCN had an obvious cell-cycle arrest at the G1-G0 phase with a decreased G2–S-phase compared to that of negative control (Figures 3(e) and 3(f), P < 0.01).

Bottom Line: Preeclampsia (PE) is a unique complication of pregnancy, the pathogenesis of which has been generally accepted to be associated with the dysfunctions of extravillous trophoblast (EVT) including proliferation, apoptosis, and migration and invasion.Also the modulations of decorin on trophoblast cells' metastasis and invasion functions were detected through regulating the matrix metalloproteinases (MMP2 and MMP9).Thus, we suggested that the contribution of decorin to the modulation of trophoblast cells might have implications for the pathogenesis of preeclampsia.

View Article: PubMed Central - PubMed

Affiliation: Department of Obstetrics and Gynecology, The First Affiliated Hospital of Nanjing Medical University, 300 Guangzhou Road, Nanjing 210029, China.

ABSTRACT
Preeclampsia (PE) is a unique complication of pregnancy, the pathogenesis of which has been generally accepted to be associated with the dysfunctions of extravillous trophoblast (EVT) including proliferation, apoptosis, and migration and invasion. Decorin (DCN) has been proved to be a decidua-derived TGF-binding proteoglycan, which negatively regulates proliferation, migration, and invasiveness of human extravillous trophoblast cells. In this study, we identified a higher expression level of decorin in severe PE placentas by both real-time reverse transcription-polymerase chain reaction (qRT-PCR) and immunohistochemistry (IHC). And an inhibitory effect of decorin on proliferation, migration, and invasion and an enhanced effect on apoptosis in trophoblast cells HTR-8/SVneo and JEG-3 were validated in vitro. Also the modulations of decorin on trophoblast cells' metastasis and invasion functions were detected through regulating the matrix metalloproteinases (MMP2 and MMP9). Thus, we suggested that the contribution of decorin to the modulation of trophoblast cells might have implications for the pathogenesis of preeclampsia.

No MeSH data available.


Related in: MedlinePlus