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Decorin-Mediated Inhibition of Human Trophoblast Cells Proliferation, Migration, and Invasion and Promotion of Apoptosis In Vitro.

Zou Y, Yu X, Lu J, Jiang Z, Zuo Q, Fan M, Huang S, Sun L - Biomed Res Int (2015)

Bottom Line: Preeclampsia (PE) is a unique complication of pregnancy, the pathogenesis of which has been generally accepted to be associated with the dysfunctions of extravillous trophoblast (EVT) including proliferation, apoptosis, and migration and invasion.Also the modulations of decorin on trophoblast cells' metastasis and invasion functions were detected through regulating the matrix metalloproteinases (MMP2 and MMP9).Thus, we suggested that the contribution of decorin to the modulation of trophoblast cells might have implications for the pathogenesis of preeclampsia.

View Article: PubMed Central - PubMed

Affiliation: Department of Obstetrics and Gynecology, The First Affiliated Hospital of Nanjing Medical University, 300 Guangzhou Road, Nanjing 210029, China.

ABSTRACT
Preeclampsia (PE) is a unique complication of pregnancy, the pathogenesis of which has been generally accepted to be associated with the dysfunctions of extravillous trophoblast (EVT) including proliferation, apoptosis, and migration and invasion. Decorin (DCN) has been proved to be a decidua-derived TGF-binding proteoglycan, which negatively regulates proliferation, migration, and invasiveness of human extravillous trophoblast cells. In this study, we identified a higher expression level of decorin in severe PE placentas by both real-time reverse transcription-polymerase chain reaction (qRT-PCR) and immunohistochemistry (IHC). And an inhibitory effect of decorin on proliferation, migration, and invasion and an enhanced effect on apoptosis in trophoblast cells HTR-8/SVneo and JEG-3 were validated in vitro. Also the modulations of decorin on trophoblast cells' metastasis and invasion functions were detected through regulating the matrix metalloproteinases (MMP2 and MMP9). Thus, we suggested that the contribution of decorin to the modulation of trophoblast cells might have implications for the pathogenesis of preeclampsia.

No MeSH data available.


Related in: MedlinePlus

The migration and invasion capacity of trophoblast cells transfected with pEGFP-DCN and control. ((a) and (b)) HTR-8/SVneo cells treated with decorin overexpression presented significantly inhibited migration (a) and invasion (b) potentials compared to control. (c) The histogram showed the statistical data of (a) and (b). (d) The migration ability of JEG-3 cells treated with decorin overexpression was significantly lower than that of the control, as determined by transwell assays. (Values are mean ± SEM; ∗P < 0.05; ∗∗P < 0.01.) (e) The invasion ability of JEG-3 cells treated with decorin overexpression was significantly lower than control. (f) The histogram showed the statistical data of (e) and (f). ((g) and (h)) Western blotting analysis of MMP2 and MMP9 protein in pEGFP-DCN or empty vector transfected HTR-8/SVneo and JEG-3 cells. (Values are mean ± SEM; ∗P < 0.05; ∗∗P < 0.01.)
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fig2: The migration and invasion capacity of trophoblast cells transfected with pEGFP-DCN and control. ((a) and (b)) HTR-8/SVneo cells treated with decorin overexpression presented significantly inhibited migration (a) and invasion (b) potentials compared to control. (c) The histogram showed the statistical data of (a) and (b). (d) The migration ability of JEG-3 cells treated with decorin overexpression was significantly lower than that of the control, as determined by transwell assays. (Values are mean ± SEM; ∗P < 0.05; ∗∗P < 0.01.) (e) The invasion ability of JEG-3 cells treated with decorin overexpression was significantly lower than control. (f) The histogram showed the statistical data of (e) and (f). ((g) and (h)) Western blotting analysis of MMP2 and MMP9 protein in pEGFP-DCN or empty vector transfected HTR-8/SVneo and JEG-3 cells. (Values are mean ± SEM; ∗P < 0.05; ∗∗P < 0.01.)

Mentions: It was determined by transwell assays that the migratory and invasive capacity of cells transfected with pEGFP-DCN reduced by approximately 37.6% and 51.88% of HTR-8/SVneo cells (Figures 2(a), 2(b), and 2(c), P < 0.01), respectively, and 57.3% and 34.8% of JEG-3 cells (Figures 2(d), 2(e), and 2(f), P < 0.01), respectively, as compared to that of control. Moreover, matrix metalloproteinases, MMP2 and MMP9, also presented a decrease under the influence of decorin overexpression in both cells (Figures 2(g) and 2(h), P < 0.01). Thus, these data proved that decorin might be closely associated with the inhibition of migration and invasion behaviors in trophoblast cells.


Decorin-Mediated Inhibition of Human Trophoblast Cells Proliferation, Migration, and Invasion and Promotion of Apoptosis In Vitro.

Zou Y, Yu X, Lu J, Jiang Z, Zuo Q, Fan M, Huang S, Sun L - Biomed Res Int (2015)

The migration and invasion capacity of trophoblast cells transfected with pEGFP-DCN and control. ((a) and (b)) HTR-8/SVneo cells treated with decorin overexpression presented significantly inhibited migration (a) and invasion (b) potentials compared to control. (c) The histogram showed the statistical data of (a) and (b). (d) The migration ability of JEG-3 cells treated with decorin overexpression was significantly lower than that of the control, as determined by transwell assays. (Values are mean ± SEM; ∗P < 0.05; ∗∗P < 0.01.) (e) The invasion ability of JEG-3 cells treated with decorin overexpression was significantly lower than control. (f) The histogram showed the statistical data of (e) and (f). ((g) and (h)) Western blotting analysis of MMP2 and MMP9 protein in pEGFP-DCN or empty vector transfected HTR-8/SVneo and JEG-3 cells. (Values are mean ± SEM; ∗P < 0.05; ∗∗P < 0.01.)
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4556865&req=5

fig2: The migration and invasion capacity of trophoblast cells transfected with pEGFP-DCN and control. ((a) and (b)) HTR-8/SVneo cells treated with decorin overexpression presented significantly inhibited migration (a) and invasion (b) potentials compared to control. (c) The histogram showed the statistical data of (a) and (b). (d) The migration ability of JEG-3 cells treated with decorin overexpression was significantly lower than that of the control, as determined by transwell assays. (Values are mean ± SEM; ∗P < 0.05; ∗∗P < 0.01.) (e) The invasion ability of JEG-3 cells treated with decorin overexpression was significantly lower than control. (f) The histogram showed the statistical data of (e) and (f). ((g) and (h)) Western blotting analysis of MMP2 and MMP9 protein in pEGFP-DCN or empty vector transfected HTR-8/SVneo and JEG-3 cells. (Values are mean ± SEM; ∗P < 0.05; ∗∗P < 0.01.)
Mentions: It was determined by transwell assays that the migratory and invasive capacity of cells transfected with pEGFP-DCN reduced by approximately 37.6% and 51.88% of HTR-8/SVneo cells (Figures 2(a), 2(b), and 2(c), P < 0.01), respectively, and 57.3% and 34.8% of JEG-3 cells (Figures 2(d), 2(e), and 2(f), P < 0.01), respectively, as compared to that of control. Moreover, matrix metalloproteinases, MMP2 and MMP9, also presented a decrease under the influence of decorin overexpression in both cells (Figures 2(g) and 2(h), P < 0.01). Thus, these data proved that decorin might be closely associated with the inhibition of migration and invasion behaviors in trophoblast cells.

Bottom Line: Preeclampsia (PE) is a unique complication of pregnancy, the pathogenesis of which has been generally accepted to be associated with the dysfunctions of extravillous trophoblast (EVT) including proliferation, apoptosis, and migration and invasion.Also the modulations of decorin on trophoblast cells' metastasis and invasion functions were detected through regulating the matrix metalloproteinases (MMP2 and MMP9).Thus, we suggested that the contribution of decorin to the modulation of trophoblast cells might have implications for the pathogenesis of preeclampsia.

View Article: PubMed Central - PubMed

Affiliation: Department of Obstetrics and Gynecology, The First Affiliated Hospital of Nanjing Medical University, 300 Guangzhou Road, Nanjing 210029, China.

ABSTRACT
Preeclampsia (PE) is a unique complication of pregnancy, the pathogenesis of which has been generally accepted to be associated with the dysfunctions of extravillous trophoblast (EVT) including proliferation, apoptosis, and migration and invasion. Decorin (DCN) has been proved to be a decidua-derived TGF-binding proteoglycan, which negatively regulates proliferation, migration, and invasiveness of human extravillous trophoblast cells. In this study, we identified a higher expression level of decorin in severe PE placentas by both real-time reverse transcription-polymerase chain reaction (qRT-PCR) and immunohistochemistry (IHC). And an inhibitory effect of decorin on proliferation, migration, and invasion and an enhanced effect on apoptosis in trophoblast cells HTR-8/SVneo and JEG-3 were validated in vitro. Also the modulations of decorin on trophoblast cells' metastasis and invasion functions were detected through regulating the matrix metalloproteinases (MMP2 and MMP9). Thus, we suggested that the contribution of decorin to the modulation of trophoblast cells might have implications for the pathogenesis of preeclampsia.

No MeSH data available.


Related in: MedlinePlus