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Decorin-Mediated Inhibition of Human Trophoblast Cells Proliferation, Migration, and Invasion and Promotion of Apoptosis In Vitro.

Zou Y, Yu X, Lu J, Jiang Z, Zuo Q, Fan M, Huang S, Sun L - Biomed Res Int (2015)

Bottom Line: Preeclampsia (PE) is a unique complication of pregnancy, the pathogenesis of which has been generally accepted to be associated with the dysfunctions of extravillous trophoblast (EVT) including proliferation, apoptosis, and migration and invasion.Also the modulations of decorin on trophoblast cells' metastasis and invasion functions were detected through regulating the matrix metalloproteinases (MMP2 and MMP9).Thus, we suggested that the contribution of decorin to the modulation of trophoblast cells might have implications for the pathogenesis of preeclampsia.

View Article: PubMed Central - PubMed

Affiliation: Department of Obstetrics and Gynecology, The First Affiliated Hospital of Nanjing Medical University, 300 Guangzhou Road, Nanjing 210029, China.

ABSTRACT
Preeclampsia (PE) is a unique complication of pregnancy, the pathogenesis of which has been generally accepted to be associated with the dysfunctions of extravillous trophoblast (EVT) including proliferation, apoptosis, and migration and invasion. Decorin (DCN) has been proved to be a decidua-derived TGF-binding proteoglycan, which negatively regulates proliferation, migration, and invasiveness of human extravillous trophoblast cells. In this study, we identified a higher expression level of decorin in severe PE placentas by both real-time reverse transcription-polymerase chain reaction (qRT-PCR) and immunohistochemistry (IHC). And an inhibitory effect of decorin on proliferation, migration, and invasion and an enhanced effect on apoptosis in trophoblast cells HTR-8/SVneo and JEG-3 were validated in vitro. Also the modulations of decorin on trophoblast cells' metastasis and invasion functions were detected through regulating the matrix metalloproteinases (MMP2 and MMP9). Thus, we suggested that the contribution of decorin to the modulation of trophoblast cells might have implications for the pathogenesis of preeclampsia.

No MeSH data available.


Related in: MedlinePlus

Expression of decorin in preeclampsia placentas compared with normal and decorin-overexpression efficiency in trophoblast cells. (a) Relative expression of decorin was 71.8% higher in preeclampsia placenta tissues compared to the normal pregnancies, as determined by qRT-PCR. (b) The mRNA expression of decorin in HTR-8/SVneo and JEG-3 cells transfected with pEGFP-DCN, detected by qRT-PCR. ((c) and (d)) The protein expression of decorin in HTR-8/SVneo and JEG-3 cells transfected with pEGFP-DCN, detected by Western blotting. Values are represented as mean ± SEM (∗∗P < 0.01).
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fig1: Expression of decorin in preeclampsia placentas compared with normal and decorin-overexpression efficiency in trophoblast cells. (a) Relative expression of decorin was 71.8% higher in preeclampsia placenta tissues compared to the normal pregnancies, as determined by qRT-PCR. (b) The mRNA expression of decorin in HTR-8/SVneo and JEG-3 cells transfected with pEGFP-DCN, detected by qRT-PCR. ((c) and (d)) The protein expression of decorin in HTR-8/SVneo and JEG-3 cells transfected with pEGFP-DCN, detected by Western blotting. Values are represented as mean ± SEM (∗∗P < 0.01).

Mentions: The expression level of decorin was detected in 9 PE and 12 normal placenta tissues by using immunohistochemical staining. The results showed that decorin protein was greatly upregulated in PE but was expressed at lower level in normal placenta tissues (Figures 1(a) and 1(b)). Also, the qRT-PCR analysis was conducted by comparing 30 PE placentas to 30 normal pregnant ones. The expression level of decorin mRNA was also significantly higher in PE placentas than that of the normal ones (Figure 1(c)). Table 1 shows the patients' clinical characteristics in detail.


Decorin-Mediated Inhibition of Human Trophoblast Cells Proliferation, Migration, and Invasion and Promotion of Apoptosis In Vitro.

Zou Y, Yu X, Lu J, Jiang Z, Zuo Q, Fan M, Huang S, Sun L - Biomed Res Int (2015)

Expression of decorin in preeclampsia placentas compared with normal and decorin-overexpression efficiency in trophoblast cells. (a) Relative expression of decorin was 71.8% higher in preeclampsia placenta tissues compared to the normal pregnancies, as determined by qRT-PCR. (b) The mRNA expression of decorin in HTR-8/SVneo and JEG-3 cells transfected with pEGFP-DCN, detected by qRT-PCR. ((c) and (d)) The protein expression of decorin in HTR-8/SVneo and JEG-3 cells transfected with pEGFP-DCN, detected by Western blotting. Values are represented as mean ± SEM (∗∗P < 0.01).
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4556865&req=5

fig1: Expression of decorin in preeclampsia placentas compared with normal and decorin-overexpression efficiency in trophoblast cells. (a) Relative expression of decorin was 71.8% higher in preeclampsia placenta tissues compared to the normal pregnancies, as determined by qRT-PCR. (b) The mRNA expression of decorin in HTR-8/SVneo and JEG-3 cells transfected with pEGFP-DCN, detected by qRT-PCR. ((c) and (d)) The protein expression of decorin in HTR-8/SVneo and JEG-3 cells transfected with pEGFP-DCN, detected by Western blotting. Values are represented as mean ± SEM (∗∗P < 0.01).
Mentions: The expression level of decorin was detected in 9 PE and 12 normal placenta tissues by using immunohistochemical staining. The results showed that decorin protein was greatly upregulated in PE but was expressed at lower level in normal placenta tissues (Figures 1(a) and 1(b)). Also, the qRT-PCR analysis was conducted by comparing 30 PE placentas to 30 normal pregnant ones. The expression level of decorin mRNA was also significantly higher in PE placentas than that of the normal ones (Figure 1(c)). Table 1 shows the patients' clinical characteristics in detail.

Bottom Line: Preeclampsia (PE) is a unique complication of pregnancy, the pathogenesis of which has been generally accepted to be associated with the dysfunctions of extravillous trophoblast (EVT) including proliferation, apoptosis, and migration and invasion.Also the modulations of decorin on trophoblast cells' metastasis and invasion functions were detected through regulating the matrix metalloproteinases (MMP2 and MMP9).Thus, we suggested that the contribution of decorin to the modulation of trophoblast cells might have implications for the pathogenesis of preeclampsia.

View Article: PubMed Central - PubMed

Affiliation: Department of Obstetrics and Gynecology, The First Affiliated Hospital of Nanjing Medical University, 300 Guangzhou Road, Nanjing 210029, China.

ABSTRACT
Preeclampsia (PE) is a unique complication of pregnancy, the pathogenesis of which has been generally accepted to be associated with the dysfunctions of extravillous trophoblast (EVT) including proliferation, apoptosis, and migration and invasion. Decorin (DCN) has been proved to be a decidua-derived TGF-binding proteoglycan, which negatively regulates proliferation, migration, and invasiveness of human extravillous trophoblast cells. In this study, we identified a higher expression level of decorin in severe PE placentas by both real-time reverse transcription-polymerase chain reaction (qRT-PCR) and immunohistochemistry (IHC). And an inhibitory effect of decorin on proliferation, migration, and invasion and an enhanced effect on apoptosis in trophoblast cells HTR-8/SVneo and JEG-3 were validated in vitro. Also the modulations of decorin on trophoblast cells' metastasis and invasion functions were detected through regulating the matrix metalloproteinases (MMP2 and MMP9). Thus, we suggested that the contribution of decorin to the modulation of trophoblast cells might have implications for the pathogenesis of preeclampsia.

No MeSH data available.


Related in: MedlinePlus