Limits...
Expression profile of six stress-related genes and productive performances of fast and slow growing broiler strains reared under heat stress conditions.

Rimoldi S, Lasagna E, Sarti FM, Marelli SP, Cozzi MC, Bernardini G, Terova G - Meta Gene (2015)

Bottom Line: CN is a naked neck slow growing broiler intended for the free range and/or organic markets, whereas RO is selected for fast growing.RO and CN broilers, 4 weeks of age, were maintained for 4 weeks at either 34 °C or 22 °C.The differences found in the mRNA copies of CASP6 gene could be partly explained by SNPs.

View Article: PubMed Central - PubMed

Affiliation: University of Insubria, Department of Biotechnology and Life Sciences, Varese, Italy.

ABSTRACT
High temperature is one of the prominent environmental factors causing economic losses to the poultry industry as it negatively affects growth and production performance in broiler chickens. We used One Step TaqMan real time RT-PCR (reverse transcription polymerase chain reaction) technology to study the effects of chronic heat stress on the expression of genes codifying for the antioxidative enzymes superoxide dismutase (SOD), and catalase (CAT), as well as for heat shock protein (HSP) 70, HSP90, glucocorticoid receptor (NR3C1), and caspase 6 (CASP6) in the liver of two different broiler genetic strains: Red JA Cou Nu Hubbard (CN) and Ross 508 Aviagen (RO). CN is a naked neck slow growing broiler intended for the free range and/or organic markets, whereas RO is selected for fast growing. We also analysed the effect of chronic heat stress on productive performances, and plasma corticosterone levels as well as the association between transcriptomic response and specific SNPs (single nucleotide polymorphisms) in each genetic strain of broiler chickens. RO and CN broilers, 4 weeks of age, were maintained for 4 weeks at either 34 °C or 22 °C. The results demonstrated that there was a genotype and a temperature main effect on the broilers' growth from the 4th to the 8th week of age, but the interaction effect between genotype and temperature resulted not statistically significant. By considering the genotype effect, fast growing broilers (RO) grew more than the slow growing ones (CN), whereas by considering the temperature effect, broilers in unheated conditions grew more than the heat stressed ones. Corticosterone levels increased significantly in the blood of heat stressed broilers, due to the activation of the HPA (hypothalamic-pituitary-adrenocortical axis). Carcass yield at slaughter was of similar values in the 4 cohorts (genotype/temperature combinations or treatment groups), ranging from 86.5 to 88.6%, whereas carcass weight was negatively influenced by heat stress in both broiler strains. Heat stress affected gene expression by downregulating CASP6 and upregulating CAT transcript levels. HSPs, SOD and NR3C1 mRNA levels remained unaffected by heat stress. The differences found in the mRNA copies of CASP6 gene could be partly explained by SNPs.

No MeSH data available.


Related in: MedlinePlus

A, B. Effect of chronic heat stress on mRNA copies of CAT and SOD genes in broilers of two breeds. Ross 308 (RO) and Red JA Cou Nu (CN) broiler genetic strains were maintained for 4 weeks (from the 4th to the 8th week of age) at temperatures of 34 °C (HT) and 22 °C (CT). Ten birds/strain/temperature were analysed and each histogram represents the average mRNA copies (means ± s.e.m. as error bar). Different small letters indicate significant differences at P ≤ 0.05.
© Copyright Policy - CC BY-NC-ND
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4556841&req=5

f0030: A, B. Effect of chronic heat stress on mRNA copies of CAT and SOD genes in broilers of two breeds. Ross 308 (RO) and Red JA Cou Nu (CN) broiler genetic strains were maintained for 4 weeks (from the 4th to the 8th week of age) at temperatures of 34 °C (HT) and 22 °C (CT). Ten birds/strain/temperature were analysed and each histogram represents the average mRNA copies (means ± s.e.m. as error bar). Different small letters indicate significant differences at P ≤ 0.05.

Mentions: The response of genes codifying for antioxidant enzymes CAT and SOD diverged between the two broiler strains following high environmental temperature exposure (Fig. 6A and B). In particular, SOD transcript abundance in the liver of two strains was not influenced by heat stress (Fig. 6B), whereas in the case of CAT gene (Fig. 6A), we found an interaction genotype–temperature effect on the expression. Indeed, CAT mRNA copy number was significantly upregulated in the liver of CN broilers maintained at HT for 4 weeks in comparison to the mRNA levels found in broilers maintained at 22 °C. It is interesting to note that the expression of CAT in the liver of heat-stressed CN broilers was not statistically different from that of RO broilers maintained at either control or heat-stressed conditions (Fig. 6A).


Expression profile of six stress-related genes and productive performances of fast and slow growing broiler strains reared under heat stress conditions.

Rimoldi S, Lasagna E, Sarti FM, Marelli SP, Cozzi MC, Bernardini G, Terova G - Meta Gene (2015)

A, B. Effect of chronic heat stress on mRNA copies of CAT and SOD genes in broilers of two breeds. Ross 308 (RO) and Red JA Cou Nu (CN) broiler genetic strains were maintained for 4 weeks (from the 4th to the 8th week of age) at temperatures of 34 °C (HT) and 22 °C (CT). Ten birds/strain/temperature were analysed and each histogram represents the average mRNA copies (means ± s.e.m. as error bar). Different small letters indicate significant differences at P ≤ 0.05.
© Copyright Policy - CC BY-NC-ND
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4556841&req=5

f0030: A, B. Effect of chronic heat stress on mRNA copies of CAT and SOD genes in broilers of two breeds. Ross 308 (RO) and Red JA Cou Nu (CN) broiler genetic strains were maintained for 4 weeks (from the 4th to the 8th week of age) at temperatures of 34 °C (HT) and 22 °C (CT). Ten birds/strain/temperature were analysed and each histogram represents the average mRNA copies (means ± s.e.m. as error bar). Different small letters indicate significant differences at P ≤ 0.05.
Mentions: The response of genes codifying for antioxidant enzymes CAT and SOD diverged between the two broiler strains following high environmental temperature exposure (Fig. 6A and B). In particular, SOD transcript abundance in the liver of two strains was not influenced by heat stress (Fig. 6B), whereas in the case of CAT gene (Fig. 6A), we found an interaction genotype–temperature effect on the expression. Indeed, CAT mRNA copy number was significantly upregulated in the liver of CN broilers maintained at HT for 4 weeks in comparison to the mRNA levels found in broilers maintained at 22 °C. It is interesting to note that the expression of CAT in the liver of heat-stressed CN broilers was not statistically different from that of RO broilers maintained at either control or heat-stressed conditions (Fig. 6A).

Bottom Line: CN is a naked neck slow growing broiler intended for the free range and/or organic markets, whereas RO is selected for fast growing.RO and CN broilers, 4 weeks of age, were maintained for 4 weeks at either 34 °C or 22 °C.The differences found in the mRNA copies of CASP6 gene could be partly explained by SNPs.

View Article: PubMed Central - PubMed

Affiliation: University of Insubria, Department of Biotechnology and Life Sciences, Varese, Italy.

ABSTRACT
High temperature is one of the prominent environmental factors causing economic losses to the poultry industry as it negatively affects growth and production performance in broiler chickens. We used One Step TaqMan real time RT-PCR (reverse transcription polymerase chain reaction) technology to study the effects of chronic heat stress on the expression of genes codifying for the antioxidative enzymes superoxide dismutase (SOD), and catalase (CAT), as well as for heat shock protein (HSP) 70, HSP90, glucocorticoid receptor (NR3C1), and caspase 6 (CASP6) in the liver of two different broiler genetic strains: Red JA Cou Nu Hubbard (CN) and Ross 508 Aviagen (RO). CN is a naked neck slow growing broiler intended for the free range and/or organic markets, whereas RO is selected for fast growing. We also analysed the effect of chronic heat stress on productive performances, and plasma corticosterone levels as well as the association between transcriptomic response and specific SNPs (single nucleotide polymorphisms) in each genetic strain of broiler chickens. RO and CN broilers, 4 weeks of age, were maintained for 4 weeks at either 34 °C or 22 °C. The results demonstrated that there was a genotype and a temperature main effect on the broilers' growth from the 4th to the 8th week of age, but the interaction effect between genotype and temperature resulted not statistically significant. By considering the genotype effect, fast growing broilers (RO) grew more than the slow growing ones (CN), whereas by considering the temperature effect, broilers in unheated conditions grew more than the heat stressed ones. Corticosterone levels increased significantly in the blood of heat stressed broilers, due to the activation of the HPA (hypothalamic-pituitary-adrenocortical axis). Carcass yield at slaughter was of similar values in the 4 cohorts (genotype/temperature combinations or treatment groups), ranging from 86.5 to 88.6%, whereas carcass weight was negatively influenced by heat stress in both broiler strains. Heat stress affected gene expression by downregulating CASP6 and upregulating CAT transcript levels. HSPs, SOD and NR3C1 mRNA levels remained unaffected by heat stress. The differences found in the mRNA copies of CASP6 gene could be partly explained by SNPs.

No MeSH data available.


Related in: MedlinePlus