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Expression profile of six stress-related genes and productive performances of fast and slow growing broiler strains reared under heat stress conditions.

Rimoldi S, Lasagna E, Sarti FM, Marelli SP, Cozzi MC, Bernardini G, Terova G - Meta Gene (2015)

Bottom Line: CN is a naked neck slow growing broiler intended for the free range and/or organic markets, whereas RO is selected for fast growing.RO and CN broilers, 4 weeks of age, were maintained for 4 weeks at either 34 °C or 22 °C.The differences found in the mRNA copies of CASP6 gene could be partly explained by SNPs.

View Article: PubMed Central - PubMed

Affiliation: University of Insubria, Department of Biotechnology and Life Sciences, Varese, Italy.

ABSTRACT
High temperature is one of the prominent environmental factors causing economic losses to the poultry industry as it negatively affects growth and production performance in broiler chickens. We used One Step TaqMan real time RT-PCR (reverse transcription polymerase chain reaction) technology to study the effects of chronic heat stress on the expression of genes codifying for the antioxidative enzymes superoxide dismutase (SOD), and catalase (CAT), as well as for heat shock protein (HSP) 70, HSP90, glucocorticoid receptor (NR3C1), and caspase 6 (CASP6) in the liver of two different broiler genetic strains: Red JA Cou Nu Hubbard (CN) and Ross 508 Aviagen (RO). CN is a naked neck slow growing broiler intended for the free range and/or organic markets, whereas RO is selected for fast growing. We also analysed the effect of chronic heat stress on productive performances, and plasma corticosterone levels as well as the association between transcriptomic response and specific SNPs (single nucleotide polymorphisms) in each genetic strain of broiler chickens. RO and CN broilers, 4 weeks of age, were maintained for 4 weeks at either 34 °C or 22 °C. The results demonstrated that there was a genotype and a temperature main effect on the broilers' growth from the 4th to the 8th week of age, but the interaction effect between genotype and temperature resulted not statistically significant. By considering the genotype effect, fast growing broilers (RO) grew more than the slow growing ones (CN), whereas by considering the temperature effect, broilers in unheated conditions grew more than the heat stressed ones. Corticosterone levels increased significantly in the blood of heat stressed broilers, due to the activation of the HPA (hypothalamic-pituitary-adrenocortical axis). Carcass yield at slaughter was of similar values in the 4 cohorts (genotype/temperature combinations or treatment groups), ranging from 86.5 to 88.6%, whereas carcass weight was negatively influenced by heat stress in both broiler strains. Heat stress affected gene expression by downregulating CASP6 and upregulating CAT transcript levels. HSPs, SOD and NR3C1 mRNA levels remained unaffected by heat stress. The differences found in the mRNA copies of CASP6 gene could be partly explained by SNPs.

No MeSH data available.


Related in: MedlinePlus

Carcass weight at slaughter of two genetic strains of broiler chickens maintained under heat stress conditions. Broilers of two different hybrid strains, Ross 308 (RO), Red JA Cou Nu (CN), were maintained from the 4th to the 8th week of age at two different environmental temperatures: 60 RO and 60 CN (in separate pens), at 34 °C (high temperature, HT), and other 60 RO and 60 CN (in separate pens), at 22 °C (control temperature, CT). The means of 10 birds/strain/temperature are shown in each histogram. Bars indicate standard error of the mean. For statistical differences, please refer to Table 5.
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f0010: Carcass weight at slaughter of two genetic strains of broiler chickens maintained under heat stress conditions. Broilers of two different hybrid strains, Ross 308 (RO), Red JA Cou Nu (CN), were maintained from the 4th to the 8th week of age at two different environmental temperatures: 60 RO and 60 CN (in separate pens), at 34 °C (high temperature, HT), and other 60 RO and 60 CN (in separate pens), at 22 °C (control temperature, CT). The means of 10 birds/strain/temperature are shown in each histogram. Bars indicate standard error of the mean. For statistical differences, please refer to Table 5.

Mentions: Carcass weight (Fig. 2) reached the highest value (2156.7 g) in RO broilers reared at 22 °C and the lowest (1422.17 g) in CN broilers reared at 34 °C. Since the interaction between temperature and genotype was not significant for this trait, we considered the main effects. The results of the statistical analysis (Table 5) considering the main effect of genotype showed that carcass weight was significantly higher in RO than in CN broiler, whereas by considering the main effect of temperature (Table 5), surprisingly, the carcasses of the unheated group (CT) resulted heavier than those of the heat stressed group. Indeed, although the difference in carcass weight between CT and HT was more than 200 g, we did not have enough evidence to be able to conclude that the difference was not due to chance.


Expression profile of six stress-related genes and productive performances of fast and slow growing broiler strains reared under heat stress conditions.

Rimoldi S, Lasagna E, Sarti FM, Marelli SP, Cozzi MC, Bernardini G, Terova G - Meta Gene (2015)

Carcass weight at slaughter of two genetic strains of broiler chickens maintained under heat stress conditions. Broilers of two different hybrid strains, Ross 308 (RO), Red JA Cou Nu (CN), were maintained from the 4th to the 8th week of age at two different environmental temperatures: 60 RO and 60 CN (in separate pens), at 34 °C (high temperature, HT), and other 60 RO and 60 CN (in separate pens), at 22 °C (control temperature, CT). The means of 10 birds/strain/temperature are shown in each histogram. Bars indicate standard error of the mean. For statistical differences, please refer to Table 5.
© Copyright Policy - CC BY-NC-ND
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4556841&req=5

f0010: Carcass weight at slaughter of two genetic strains of broiler chickens maintained under heat stress conditions. Broilers of two different hybrid strains, Ross 308 (RO), Red JA Cou Nu (CN), were maintained from the 4th to the 8th week of age at two different environmental temperatures: 60 RO and 60 CN (in separate pens), at 34 °C (high temperature, HT), and other 60 RO and 60 CN (in separate pens), at 22 °C (control temperature, CT). The means of 10 birds/strain/temperature are shown in each histogram. Bars indicate standard error of the mean. For statistical differences, please refer to Table 5.
Mentions: Carcass weight (Fig. 2) reached the highest value (2156.7 g) in RO broilers reared at 22 °C and the lowest (1422.17 g) in CN broilers reared at 34 °C. Since the interaction between temperature and genotype was not significant for this trait, we considered the main effects. The results of the statistical analysis (Table 5) considering the main effect of genotype showed that carcass weight was significantly higher in RO than in CN broiler, whereas by considering the main effect of temperature (Table 5), surprisingly, the carcasses of the unheated group (CT) resulted heavier than those of the heat stressed group. Indeed, although the difference in carcass weight between CT and HT was more than 200 g, we did not have enough evidence to be able to conclude that the difference was not due to chance.

Bottom Line: CN is a naked neck slow growing broiler intended for the free range and/or organic markets, whereas RO is selected for fast growing.RO and CN broilers, 4 weeks of age, were maintained for 4 weeks at either 34 °C or 22 °C.The differences found in the mRNA copies of CASP6 gene could be partly explained by SNPs.

View Article: PubMed Central - PubMed

Affiliation: University of Insubria, Department of Biotechnology and Life Sciences, Varese, Italy.

ABSTRACT
High temperature is one of the prominent environmental factors causing economic losses to the poultry industry as it negatively affects growth and production performance in broiler chickens. We used One Step TaqMan real time RT-PCR (reverse transcription polymerase chain reaction) technology to study the effects of chronic heat stress on the expression of genes codifying for the antioxidative enzymes superoxide dismutase (SOD), and catalase (CAT), as well as for heat shock protein (HSP) 70, HSP90, glucocorticoid receptor (NR3C1), and caspase 6 (CASP6) in the liver of two different broiler genetic strains: Red JA Cou Nu Hubbard (CN) and Ross 508 Aviagen (RO). CN is a naked neck slow growing broiler intended for the free range and/or organic markets, whereas RO is selected for fast growing. We also analysed the effect of chronic heat stress on productive performances, and plasma corticosterone levels as well as the association between transcriptomic response and specific SNPs (single nucleotide polymorphisms) in each genetic strain of broiler chickens. RO and CN broilers, 4 weeks of age, were maintained for 4 weeks at either 34 °C or 22 °C. The results demonstrated that there was a genotype and a temperature main effect on the broilers' growth from the 4th to the 8th week of age, but the interaction effect between genotype and temperature resulted not statistically significant. By considering the genotype effect, fast growing broilers (RO) grew more than the slow growing ones (CN), whereas by considering the temperature effect, broilers in unheated conditions grew more than the heat stressed ones. Corticosterone levels increased significantly in the blood of heat stressed broilers, due to the activation of the HPA (hypothalamic-pituitary-adrenocortical axis). Carcass yield at slaughter was of similar values in the 4 cohorts (genotype/temperature combinations or treatment groups), ranging from 86.5 to 88.6%, whereas carcass weight was negatively influenced by heat stress in both broiler strains. Heat stress affected gene expression by downregulating CASP6 and upregulating CAT transcript levels. HSPs, SOD and NR3C1 mRNA levels remained unaffected by heat stress. The differences found in the mRNA copies of CASP6 gene could be partly explained by SNPs.

No MeSH data available.


Related in: MedlinePlus