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Effects of Mitochondrial Uncoupling Protein 2 Inhibition by Genipin in Human Cumulus Cells.

Ge H, Zhang F, Shan D, Chen H, Wang X, Ling C, Xi H, Huang J, Zhu C, Lv J - Biomed Res Int (2015)

Bottom Line: There were no significant differences in ATP and mitochondrial membrane potential levels in cumulus cells from UCP2 inhibiting groups as compared with the control.However, the ratio of reduced GSH to GSSG significantly declined after treatment with Genipin.But there was no significant difference in estradiol concentrations.

View Article: PubMed Central - PubMed

Affiliation: Reproductive Health Center, Second Affiliated Hospital of Wenzhou Medical University, 109 Xueyuan Road, Wenzhou, Zhejiang 325000, China.

ABSTRACT
UCP2 plays a physiological role by regulating mitochondrial biogenesis, maintaining energy balance, ROS elimination, and regulating cellular autophagy in numerous tissues. But the exact roles of UCP2 in cumulus cells are still not clear. Genipin, a special UCP2 inhibitor, was added into the cultural medium to explore the roles of UCP2 in human cumulus cells. There were no significant differences in ATP and mitochondrial membrane potential levels in cumulus cells from UCP2 inhibiting groups as compared with the control. The levels of ROS and Mn-SOD were markedly elevated after UCP2 inhibited Genipin. However, the ratio of reduced GSH to GSSG significantly declined after treatment with Genipin. UCP2 inhibition by Genipin also resulted in obvious increase in the active caspase-3, which accompanied the decline of caspase-3 mRNA. The level of progesterone in culture medium declined obviously after Genipin treatment. But there was no significant difference in estradiol concentrations. This study indicated that UCP2 is expressed in human cumulus cells and plays important roles on mediate ROS production, apoptotic process, and steroidogenesis, suggesting UCP2 may be involved in regulation of follicle development and oocyte maturation and quality.

No MeSH data available.


Effects of UCP2 inhibited by Genipin on steroidogenesis. (a) A slight decline of concentrations of estradiol in culture medium was found, but no significant difference was detected between UCP2 inhibited by Genipin and the control. (b) The level of progesterone in culture medium decline obviously after Genipin treated. Con means control, asterisks indicate significant difference between control and treatment group at P < 0.05.
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fig4: Effects of UCP2 inhibited by Genipin on steroidogenesis. (a) A slight decline of concentrations of estradiol in culture medium was found, but no significant difference was detected between UCP2 inhibited by Genipin and the control. (b) The level of progesterone in culture medium decline obviously after Genipin treated. Con means control, asterisks indicate significant difference between control and treatment group at P < 0.05.

Mentions: To evaluate the effects of UCP2 on steroid hormone secretion of CCs in vitro, the concentrations of estradiol and progesterone in culture medium were measured. A decline of concentrations of estradiol was found, but no significant difference was detected in all Genipin treated groups as compared with control (P > 0.05, Figure 4(a)). On the contrary, the level of progesterone in culture medium has a decline tendency after Genipin treatment, and significant differences were detected in 20 and 50 μM Genipin treated groups (P < 0.05, Figure 4(b)).


Effects of Mitochondrial Uncoupling Protein 2 Inhibition by Genipin in Human Cumulus Cells.

Ge H, Zhang F, Shan D, Chen H, Wang X, Ling C, Xi H, Huang J, Zhu C, Lv J - Biomed Res Int (2015)

Effects of UCP2 inhibited by Genipin on steroidogenesis. (a) A slight decline of concentrations of estradiol in culture medium was found, but no significant difference was detected between UCP2 inhibited by Genipin and the control. (b) The level of progesterone in culture medium decline obviously after Genipin treated. Con means control, asterisks indicate significant difference between control and treatment group at P < 0.05.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4556840&req=5

fig4: Effects of UCP2 inhibited by Genipin on steroidogenesis. (a) A slight decline of concentrations of estradiol in culture medium was found, but no significant difference was detected between UCP2 inhibited by Genipin and the control. (b) The level of progesterone in culture medium decline obviously after Genipin treated. Con means control, asterisks indicate significant difference between control and treatment group at P < 0.05.
Mentions: To evaluate the effects of UCP2 on steroid hormone secretion of CCs in vitro, the concentrations of estradiol and progesterone in culture medium were measured. A decline of concentrations of estradiol was found, but no significant difference was detected in all Genipin treated groups as compared with control (P > 0.05, Figure 4(a)). On the contrary, the level of progesterone in culture medium has a decline tendency after Genipin treatment, and significant differences were detected in 20 and 50 μM Genipin treated groups (P < 0.05, Figure 4(b)).

Bottom Line: There were no significant differences in ATP and mitochondrial membrane potential levels in cumulus cells from UCP2 inhibiting groups as compared with the control.However, the ratio of reduced GSH to GSSG significantly declined after treatment with Genipin.But there was no significant difference in estradiol concentrations.

View Article: PubMed Central - PubMed

Affiliation: Reproductive Health Center, Second Affiliated Hospital of Wenzhou Medical University, 109 Xueyuan Road, Wenzhou, Zhejiang 325000, China.

ABSTRACT
UCP2 plays a physiological role by regulating mitochondrial biogenesis, maintaining energy balance, ROS elimination, and regulating cellular autophagy in numerous tissues. But the exact roles of UCP2 in cumulus cells are still not clear. Genipin, a special UCP2 inhibitor, was added into the cultural medium to explore the roles of UCP2 in human cumulus cells. There were no significant differences in ATP and mitochondrial membrane potential levels in cumulus cells from UCP2 inhibiting groups as compared with the control. The levels of ROS and Mn-SOD were markedly elevated after UCP2 inhibited Genipin. However, the ratio of reduced GSH to GSSG significantly declined after treatment with Genipin. UCP2 inhibition by Genipin also resulted in obvious increase in the active caspase-3, which accompanied the decline of caspase-3 mRNA. The level of progesterone in culture medium declined obviously after Genipin treatment. But there was no significant difference in estradiol concentrations. This study indicated that UCP2 is expressed in human cumulus cells and plays important roles on mediate ROS production, apoptotic process, and steroidogenesis, suggesting UCP2 may be involved in regulation of follicle development and oocyte maturation and quality.

No MeSH data available.