Limits...
MiR-183 Regulates ITGB1P Expression and Promotes Invasion of Endometrial Stromal Cells.

Chen J, Gu L, Ni J, Hu P, Hu K, Shi YL - Biomed Res Int (2015)

Bottom Line: We applied in the previous study miRNA microarray screening analysis to identify several differentially expressed miRNAs, including miR-183 in normal, eutopic, and ectopic endometrium.Western blotting analyses showed that integrin β1 (ITGB1), but not AMIGO2, was affected by miR-183 overexpression, whereas no protein expression of VAV3 and PSEN2 was detected.Moreover, we found that ITGB1 is overexpressed in the endometrium of endometriosis patients.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory of Reproductive Medicine, Department of Gynecology, Nanjing Maternity and Child Health Care Hospital Affiliated to Nanjing Medical University, 123 Tianfei Road, Nanjing, Jiangsu 210029, China.

ABSTRACT
We applied in the previous study miRNA microarray screening analysis to identify several differentially expressed miRNAs, including miR-183 in normal, eutopic, and ectopic endometrium. Knockdown of miR-183 expression induced the invasiveness and inhibition of apoptosis in endometrial stromal cells. The current study aims to identify the miR-183 targets with relevance to cell functions in endometrial stromal cells, to verify the interaction of miR-183 with its target genes, and to confirm the role of miR-183 in the process of endometriosis. Using microarray analysis, we identified 27 differentially expressed genes (19 were upregulated and 8 downregulated), from which we selected 4 downregulated genes (ITGB1, AMIGO2, VAV3, and PSEN2) based on GO databases for functional analysis and significant pathway analysis. Western blotting analyses showed that integrin β1 (ITGB1), but not AMIGO2, was affected by miR-183 overexpression, whereas no protein expression of VAV3 and PSEN2 was detected. Luciferase reporter assay verified that ITGB1 is a target gene of miR-183. Moreover, we found that ITGB1 is overexpressed in the endometrium of endometriosis patients. Furthermore, overexpression of ITGB1 rescued the repressive effects of miR-183 on the invasiveness of endometrial stromal cells. These findings, together with the fact that ITGB1 is a critical factor for cell adhesion and invasiveness, suggest that miR-183 may be involved in the development of endometriosis by regulating ITGB1 in endometrial stromal cells.

No MeSH data available.


Related in: MedlinePlus

Validation of ITGB1 expression in eutopic endometrium with endometriosis (n = 19) versus normal group (n = 18) by qPCR. The results were analyzed using the comparative threshold cycle (CT) method. Gene expression data is presented as the fold of change relative to the levels of reference gene. ∗P < 0.05 when compared to the normal group. Error bars represent ± SEM.
© Copyright Policy
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4556833&req=5

fig4: Validation of ITGB1 expression in eutopic endometrium with endometriosis (n = 19) versus normal group (n = 18) by qPCR. The results were analyzed using the comparative threshold cycle (CT) method. Gene expression data is presented as the fold of change relative to the levels of reference gene. ∗P < 0.05 when compared to the normal group. Error bars represent ± SEM.

Mentions: Real-time RT-PCR results indicated that ITGB was significantly increased in the eutopic endometrial tissues from patients with endometriosis (n = 19) compared with endometrium from normal women (n = 18) (P < 0.05) (Figure 4).


MiR-183 Regulates ITGB1P Expression and Promotes Invasion of Endometrial Stromal Cells.

Chen J, Gu L, Ni J, Hu P, Hu K, Shi YL - Biomed Res Int (2015)

Validation of ITGB1 expression in eutopic endometrium with endometriosis (n = 19) versus normal group (n = 18) by qPCR. The results were analyzed using the comparative threshold cycle (CT) method. Gene expression data is presented as the fold of change relative to the levels of reference gene. ∗P < 0.05 when compared to the normal group. Error bars represent ± SEM.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4556833&req=5

fig4: Validation of ITGB1 expression in eutopic endometrium with endometriosis (n = 19) versus normal group (n = 18) by qPCR. The results were analyzed using the comparative threshold cycle (CT) method. Gene expression data is presented as the fold of change relative to the levels of reference gene. ∗P < 0.05 when compared to the normal group. Error bars represent ± SEM.
Mentions: Real-time RT-PCR results indicated that ITGB was significantly increased in the eutopic endometrial tissues from patients with endometriosis (n = 19) compared with endometrium from normal women (n = 18) (P < 0.05) (Figure 4).

Bottom Line: We applied in the previous study miRNA microarray screening analysis to identify several differentially expressed miRNAs, including miR-183 in normal, eutopic, and ectopic endometrium.Western blotting analyses showed that integrin β1 (ITGB1), but not AMIGO2, was affected by miR-183 overexpression, whereas no protein expression of VAV3 and PSEN2 was detected.Moreover, we found that ITGB1 is overexpressed in the endometrium of endometriosis patients.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory of Reproductive Medicine, Department of Gynecology, Nanjing Maternity and Child Health Care Hospital Affiliated to Nanjing Medical University, 123 Tianfei Road, Nanjing, Jiangsu 210029, China.

ABSTRACT
We applied in the previous study miRNA microarray screening analysis to identify several differentially expressed miRNAs, including miR-183 in normal, eutopic, and ectopic endometrium. Knockdown of miR-183 expression induced the invasiveness and inhibition of apoptosis in endometrial stromal cells. The current study aims to identify the miR-183 targets with relevance to cell functions in endometrial stromal cells, to verify the interaction of miR-183 with its target genes, and to confirm the role of miR-183 in the process of endometriosis. Using microarray analysis, we identified 27 differentially expressed genes (19 were upregulated and 8 downregulated), from which we selected 4 downregulated genes (ITGB1, AMIGO2, VAV3, and PSEN2) based on GO databases for functional analysis and significant pathway analysis. Western blotting analyses showed that integrin β1 (ITGB1), but not AMIGO2, was affected by miR-183 overexpression, whereas no protein expression of VAV3 and PSEN2 was detected. Luciferase reporter assay verified that ITGB1 is a target gene of miR-183. Moreover, we found that ITGB1 is overexpressed in the endometrium of endometriosis patients. Furthermore, overexpression of ITGB1 rescued the repressive effects of miR-183 on the invasiveness of endometrial stromal cells. These findings, together with the fact that ITGB1 is a critical factor for cell adhesion and invasiveness, suggest that miR-183 may be involved in the development of endometriosis by regulating ITGB1 in endometrial stromal cells.

No MeSH data available.


Related in: MedlinePlus