Limits...
Chemical Composition and Vasorelaxant and Antispasmodic Effects of Essential Oil from Rosa indica L. Petals.

Rasheed HM, Khan T, Wahid F, Khan R, Shah AJ - Evid Based Complement Alternat Med (2015)

Bottom Line: Therefore, the basic aim of this study was to explore the chemical composition and gastrointestinal and cardiovascular effects of the essential oil obtained from R. indica.The cardiovascular and gastrointestinal effects were investigated using electrophysiological measurements.Considering these evidences, it can be concluded that R. indica essential oil may work as a complementary and alternative medicine in gastrointestinal and cardiovascular diseases.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmacy, COMSATS Institute of Information Technology, Abbottabad 22060, Pakistan.

ABSTRACT
Rosa indica L. belongs to the family Rosaceae and is locally known as gulaab. It has different traditional uses in cardiovascular and gastrointestinal disorders but there is no scientific data available in this regard. Therefore, the basic aim of this study was to explore the chemical composition and gastrointestinal and cardiovascular effects of the essential oil obtained from R. indica. The chemical composition of the essential oil was investigated using gas chromatography-mass spectrometry (GC-MS) technique. The cardiovascular and gastrointestinal effects were investigated using electrophysiological measurements. The GC-MS analysis of the essential oil showed various chemical components including acetic acid, mercaptohexyl ester, butanoic acid, 2-methyl-5-oxo-1-cyclopentene-1-yl ester, artemiseole, methyl santonilate, isosteviol, caryophyllene oxide, pentyl phenyl acetate, dihydromyrcene, 1,5-octadecadien, octadecanoic acid, ethyl ester, palmitic acid (2-phenyl-1,3-dioxolan-4-yl methyl ester), santolina epoxide, and 9-farnesene. The electrophysiological measurements revealed that essential oil was more potent against K(+) (80 mM) than phenylephrine precontractions using isolated rabbit aorta preparations. In isolated rabbit jejunum preparations, it showed more potency against high K(+) induced contractions than spontaneous contractions. Considering these evidences, it can be concluded that R. indica essential oil may work as a complementary and alternative medicine in gastrointestinal and cardiovascular diseases.

No MeSH data available.


Related in: MedlinePlus

Typical tracing of the (a) normal contractions and relaxation, (b) concentration-dependent spasmolytic effect of essential oil isolated from Rosa indica (Ri. Eoil), and (c) verapamil on spontaneously contracted isolated rabbit jejunum preparations.
© Copyright Policy - open-access
Related In: Results  -  Collection


getmorefigures.php?uid=PMC4556831&req=5

fig4: Typical tracing of the (a) normal contractions and relaxation, (b) concentration-dependent spasmolytic effect of essential oil isolated from Rosa indica (Ri. Eoil), and (c) verapamil on spontaneously contracted isolated rabbit jejunum preparations.

Mentions: The spasmolytic effect of essential oil derived from R. indica was measured at different dose levels in cumulative manner (0.01–1 mg/mL) using isolated jejunum of rabbit. The effect was started at 0.01 mg/mL against spontaneous contractions and tissue was completely relaxed at 1.0 mg/mL dose (Figures 4(b) and 5(a)). Normal contractions and relaxation (spontaneous contraction) are shown in Figure 4(a). The calculated (n = 3) effective concentration (EC50) of essential oil for spontaneous and high K+ was 0.418 mg/mL (0.3–0.536) and 0.298 (0.1–0.496), respectively. The spasmolytic effect for the essential oil (Figure 4(b)) was also compared with verapamil (standard drug) (Figures 4(c) and 5(b)). The effective concentration (EC50) of verapamil against spontaneous and high K+ was 0.0539 mg/mL (0.03–0.0778) and 0.0236 (0.01–0.0372), respectively. The comparative results showed that essential oil has verapamil-like smooth muscle relaxing or spasmolytic activity.


Chemical Composition and Vasorelaxant and Antispasmodic Effects of Essential Oil from Rosa indica L. Petals.

Rasheed HM, Khan T, Wahid F, Khan R, Shah AJ - Evid Based Complement Alternat Med (2015)

Typical tracing of the (a) normal contractions and relaxation, (b) concentration-dependent spasmolytic effect of essential oil isolated from Rosa indica (Ri. Eoil), and (c) verapamil on spontaneously contracted isolated rabbit jejunum preparations.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4556831&req=5

fig4: Typical tracing of the (a) normal contractions and relaxation, (b) concentration-dependent spasmolytic effect of essential oil isolated from Rosa indica (Ri. Eoil), and (c) verapamil on spontaneously contracted isolated rabbit jejunum preparations.
Mentions: The spasmolytic effect of essential oil derived from R. indica was measured at different dose levels in cumulative manner (0.01–1 mg/mL) using isolated jejunum of rabbit. The effect was started at 0.01 mg/mL against spontaneous contractions and tissue was completely relaxed at 1.0 mg/mL dose (Figures 4(b) and 5(a)). Normal contractions and relaxation (spontaneous contraction) are shown in Figure 4(a). The calculated (n = 3) effective concentration (EC50) of essential oil for spontaneous and high K+ was 0.418 mg/mL (0.3–0.536) and 0.298 (0.1–0.496), respectively. The spasmolytic effect for the essential oil (Figure 4(b)) was also compared with verapamil (standard drug) (Figures 4(c) and 5(b)). The effective concentration (EC50) of verapamil against spontaneous and high K+ was 0.0539 mg/mL (0.03–0.0778) and 0.0236 (0.01–0.0372), respectively. The comparative results showed that essential oil has verapamil-like smooth muscle relaxing or spasmolytic activity.

Bottom Line: Therefore, the basic aim of this study was to explore the chemical composition and gastrointestinal and cardiovascular effects of the essential oil obtained from R. indica.The cardiovascular and gastrointestinal effects were investigated using electrophysiological measurements.Considering these evidences, it can be concluded that R. indica essential oil may work as a complementary and alternative medicine in gastrointestinal and cardiovascular diseases.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmacy, COMSATS Institute of Information Technology, Abbottabad 22060, Pakistan.

ABSTRACT
Rosa indica L. belongs to the family Rosaceae and is locally known as gulaab. It has different traditional uses in cardiovascular and gastrointestinal disorders but there is no scientific data available in this regard. Therefore, the basic aim of this study was to explore the chemical composition and gastrointestinal and cardiovascular effects of the essential oil obtained from R. indica. The chemical composition of the essential oil was investigated using gas chromatography-mass spectrometry (GC-MS) technique. The cardiovascular and gastrointestinal effects were investigated using electrophysiological measurements. The GC-MS analysis of the essential oil showed various chemical components including acetic acid, mercaptohexyl ester, butanoic acid, 2-methyl-5-oxo-1-cyclopentene-1-yl ester, artemiseole, methyl santonilate, isosteviol, caryophyllene oxide, pentyl phenyl acetate, dihydromyrcene, 1,5-octadecadien, octadecanoic acid, ethyl ester, palmitic acid (2-phenyl-1,3-dioxolan-4-yl methyl ester), santolina epoxide, and 9-farnesene. The electrophysiological measurements revealed that essential oil was more potent against K(+) (80 mM) than phenylephrine precontractions using isolated rabbit aorta preparations. In isolated rabbit jejunum preparations, it showed more potency against high K(+) induced contractions than spontaneous contractions. Considering these evidences, it can be concluded that R. indica essential oil may work as a complementary and alternative medicine in gastrointestinal and cardiovascular diseases.

No MeSH data available.


Related in: MedlinePlus