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Chemical Composition and Vasorelaxant and Antispasmodic Effects of Essential Oil from Rosa indica L. Petals.

Rasheed HM, Khan T, Wahid F, Khan R, Shah AJ - Evid Based Complement Alternat Med (2015)

Bottom Line: Therefore, the basic aim of this study was to explore the chemical composition and gastrointestinal and cardiovascular effects of the essential oil obtained from R. indica.The cardiovascular and gastrointestinal effects were investigated using electrophysiological measurements.Considering these evidences, it can be concluded that R. indica essential oil may work as a complementary and alternative medicine in gastrointestinal and cardiovascular diseases.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmacy, COMSATS Institute of Information Technology, Abbottabad 22060, Pakistan.

ABSTRACT
Rosa indica L. belongs to the family Rosaceae and is locally known as gulaab. It has different traditional uses in cardiovascular and gastrointestinal disorders but there is no scientific data available in this regard. Therefore, the basic aim of this study was to explore the chemical composition and gastrointestinal and cardiovascular effects of the essential oil obtained from R. indica. The chemical composition of the essential oil was investigated using gas chromatography-mass spectrometry (GC-MS) technique. The cardiovascular and gastrointestinal effects were investigated using electrophysiological measurements. The GC-MS analysis of the essential oil showed various chemical components including acetic acid, mercaptohexyl ester, butanoic acid, 2-methyl-5-oxo-1-cyclopentene-1-yl ester, artemiseole, methyl santonilate, isosteviol, caryophyllene oxide, pentyl phenyl acetate, dihydromyrcene, 1,5-octadecadien, octadecanoic acid, ethyl ester, palmitic acid (2-phenyl-1,3-dioxolan-4-yl methyl ester), santolina epoxide, and 9-farnesene. The electrophysiological measurements revealed that essential oil was more potent against K(+) (80 mM) than phenylephrine precontractions using isolated rabbit aorta preparations. In isolated rabbit jejunum preparations, it showed more potency against high K(+) induced contractions than spontaneous contractions. Considering these evidences, it can be concluded that R. indica essential oil may work as a complementary and alternative medicine in gastrointestinal and cardiovascular diseases.

No MeSH data available.


Related in: MedlinePlus

Typical graph shows the concentration-response curve of the essential oil from fresh petals of R. indica (Ri. Eoil) (a) and verapamil (1 μM) (b) on K+ (80 mM) and phenylephrine (1 μM) (PE) induced vasoconstrictions in isolated rabbit aorta preparations. Values shown are mean ± SEM (n = 3).
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fig3: Typical graph shows the concentration-response curve of the essential oil from fresh petals of R. indica (Ri. Eoil) (a) and verapamil (1 μM) (b) on K+ (80 mM) and phenylephrine (1 μM) (PE) induced vasoconstrictions in isolated rabbit aorta preparations. Values shown are mean ± SEM (n = 3).

Mentions: The vasorelaxant effect of the essential oil derived from fresh petals of R. indica was studied on aorta rings. The results showed that vasorelaxant effects were produced by the essential oil against PE and high K+. These results were compared with the vasorelaxant effect produced by verapamil against PE and K+ (80 mM) induced contractions (Figure 3(b)), thus showing vasorelaxant effect on rabbit aortic ring. As shown in Figures 2 and 3(a), the vasorelaxant effect of essential oil was started at 3 mg/mL against high K+ and 0.01 mg/mL for PE and the maximum effect was produced at 10 mg/mL against the contractions produced by both high K+ and PE. The median effective concentration (EC50) of essential oil for PE and high K+ is 7.39 mg/mL (5.0–9.78) and 5.80 (5.0–6.6). The median effective concentration (EC50) of verapamil is 0.58 mg/mL (0.3–0.86) and 0.0455 (0.03–0.061) on PE and high K+ induced contractions, respectively.


Chemical Composition and Vasorelaxant and Antispasmodic Effects of Essential Oil from Rosa indica L. Petals.

Rasheed HM, Khan T, Wahid F, Khan R, Shah AJ - Evid Based Complement Alternat Med (2015)

Typical graph shows the concentration-response curve of the essential oil from fresh petals of R. indica (Ri. Eoil) (a) and verapamil (1 μM) (b) on K+ (80 mM) and phenylephrine (1 μM) (PE) induced vasoconstrictions in isolated rabbit aorta preparations. Values shown are mean ± SEM (n = 3).
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4556831&req=5

fig3: Typical graph shows the concentration-response curve of the essential oil from fresh petals of R. indica (Ri. Eoil) (a) and verapamil (1 μM) (b) on K+ (80 mM) and phenylephrine (1 μM) (PE) induced vasoconstrictions in isolated rabbit aorta preparations. Values shown are mean ± SEM (n = 3).
Mentions: The vasorelaxant effect of the essential oil derived from fresh petals of R. indica was studied on aorta rings. The results showed that vasorelaxant effects were produced by the essential oil against PE and high K+. These results were compared with the vasorelaxant effect produced by verapamil against PE and K+ (80 mM) induced contractions (Figure 3(b)), thus showing vasorelaxant effect on rabbit aortic ring. As shown in Figures 2 and 3(a), the vasorelaxant effect of essential oil was started at 3 mg/mL against high K+ and 0.01 mg/mL for PE and the maximum effect was produced at 10 mg/mL against the contractions produced by both high K+ and PE. The median effective concentration (EC50) of essential oil for PE and high K+ is 7.39 mg/mL (5.0–9.78) and 5.80 (5.0–6.6). The median effective concentration (EC50) of verapamil is 0.58 mg/mL (0.3–0.86) and 0.0455 (0.03–0.061) on PE and high K+ induced contractions, respectively.

Bottom Line: Therefore, the basic aim of this study was to explore the chemical composition and gastrointestinal and cardiovascular effects of the essential oil obtained from R. indica.The cardiovascular and gastrointestinal effects were investigated using electrophysiological measurements.Considering these evidences, it can be concluded that R. indica essential oil may work as a complementary and alternative medicine in gastrointestinal and cardiovascular diseases.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmacy, COMSATS Institute of Information Technology, Abbottabad 22060, Pakistan.

ABSTRACT
Rosa indica L. belongs to the family Rosaceae and is locally known as gulaab. It has different traditional uses in cardiovascular and gastrointestinal disorders but there is no scientific data available in this regard. Therefore, the basic aim of this study was to explore the chemical composition and gastrointestinal and cardiovascular effects of the essential oil obtained from R. indica. The chemical composition of the essential oil was investigated using gas chromatography-mass spectrometry (GC-MS) technique. The cardiovascular and gastrointestinal effects were investigated using electrophysiological measurements. The GC-MS analysis of the essential oil showed various chemical components including acetic acid, mercaptohexyl ester, butanoic acid, 2-methyl-5-oxo-1-cyclopentene-1-yl ester, artemiseole, methyl santonilate, isosteviol, caryophyllene oxide, pentyl phenyl acetate, dihydromyrcene, 1,5-octadecadien, octadecanoic acid, ethyl ester, palmitic acid (2-phenyl-1,3-dioxolan-4-yl methyl ester), santolina epoxide, and 9-farnesene. The electrophysiological measurements revealed that essential oil was more potent against K(+) (80 mM) than phenylephrine precontractions using isolated rabbit aorta preparations. In isolated rabbit jejunum preparations, it showed more potency against high K(+) induced contractions than spontaneous contractions. Considering these evidences, it can be concluded that R. indica essential oil may work as a complementary and alternative medicine in gastrointestinal and cardiovascular diseases.

No MeSH data available.


Related in: MedlinePlus