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Paracrine Regulation of Steroidogenesis in Theca Cells by Granulosa Cells Derived from Mouse Preantral Follicles.

Liu X, Qiao P, Jiang A, Jiang J, Han H, Wang L, Ren C - Biomed Res Int (2015)

Bottom Line: Interaction partners of follicular cells play a significant role in steroidogenesis, follicular formation, and development.Thus, GCs were capable of promoting steroidogenesis and LH responsiveness in TCs.This study provided a basis for further exploration of ovarian endocrine mechanism and pathologies.

View Article: PubMed Central - PubMed

Affiliation: Clinical Center of Reproductive Medicine, Affiliated Hospital of Weifang Medical University, Weifang, Shandong 261031, China.

ABSTRACT
Interaction partners of follicular cells play a significant role in steroidogenesis, follicular formation, and development. Androgen secreted by theca cells (TCs) can initiate follicle development and ovulation and provide precursor materials for estrogen synthesis. Therefore, studies on ovarian microenvironment will not only lead to better understanding of the steroidogenesis but also have clinical significance for ovarian endocrine abnormalities such as hyperandrogenism in polycystic ovary syndrome (PCOS). This study applied the Transwell coculture model to investigate if the interaction between granulosa and theca cells may affect androgen production in theca cells. Concentrations of testosterone and androstenedione in the spent medium were measured by radioimmunoassay and enzyme linked immunosorbent assay, respectively. The results show that the coculture with granulosa cells (GCs) increases steroidogenesis in TCs. In addition, testosterone and androstenedione productions in response to LH stimulation were also increased in the coculture model. Significantly increased mRNA expressions of steroidogenic enzymes (Star, Cyp11a1, Cyp17a1, and Hsd3b2) were observed in the cocultured TCs. Thus, GCs were capable of promoting steroidogenesis and LH responsiveness in TCs. This study provided a basis for further exploration of ovarian endocrine mechanism and pathologies.

No MeSH data available.


Related in: MedlinePlus

(a) Effect of granulosa cells on mRNA expression of LHR in TCs cells and LH stimulation of androgen production in TCs in the cocultured model. The LHR mRNA expression of TCs in cocultured model increased significantly at 48 h but manifested no difference compared with TCs cultured alone at 24 h and 72 h (GAPDH as internal control). (b) Effect of GCs on testosterone production responded to LH in TCs cells after being cocultured for 48 h. Testosterone production significantly increased in response to low concentrations of LH (0–0.1 IU/mL) in the cocultured model but no increase was observed at high concentration (1 IU/mL). (c) Effect of GCs on androstenedione production responded to LH in TCs cells after being cocultured for 48 h. Androstenedione production significantly increased in response to different concentrations of LH (0-1 IU/mL) in the cocultured model. Data were expressed as mean ± SEM of three independent experiments. The asterisk showed the statistically significant difference.   ∗P < 0.05.
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fig4: (a) Effect of granulosa cells on mRNA expression of LHR in TCs cells and LH stimulation of androgen production in TCs in the cocultured model. The LHR mRNA expression of TCs in cocultured model increased significantly at 48 h but manifested no difference compared with TCs cultured alone at 24 h and 72 h (GAPDH as internal control). (b) Effect of GCs on testosterone production responded to LH in TCs cells after being cocultured for 48 h. Testosterone production significantly increased in response to low concentrations of LH (0–0.1 IU/mL) in the cocultured model but no increase was observed at high concentration (1 IU/mL). (c) Effect of GCs on androstenedione production responded to LH in TCs cells after being cocultured for 48 h. Androstenedione production significantly increased in response to different concentrations of LH (0-1 IU/mL) in the cocultured model. Data were expressed as mean ± SEM of three independent experiments. The asterisk showed the statistically significant difference.   ∗P < 0.05.

Mentions: While the LHR mRNA expression of TCs in the cocultured model manifested no difference compared with TCs cultured alone at 24 h (P > 0.05, Figure 4(a)), at 48 h the LHR mRNA expression of TCs increased significantly (P < 0.05, Figure 4(a)), indicating that paracrine factors secreted from GC cells could stimulate the LHR mRNA expression in TCs. The subsequent disappearance of difference between TCs cultured alone and cocultured model may reflect the suboptimal conditions related to in vitro environment. The cocultured GCs could enhance the capacity of LH responsiveness through increasing LHR mRNA expression in TCs.


Paracrine Regulation of Steroidogenesis in Theca Cells by Granulosa Cells Derived from Mouse Preantral Follicles.

Liu X, Qiao P, Jiang A, Jiang J, Han H, Wang L, Ren C - Biomed Res Int (2015)

(a) Effect of granulosa cells on mRNA expression of LHR in TCs cells and LH stimulation of androgen production in TCs in the cocultured model. The LHR mRNA expression of TCs in cocultured model increased significantly at 48 h but manifested no difference compared with TCs cultured alone at 24 h and 72 h (GAPDH as internal control). (b) Effect of GCs on testosterone production responded to LH in TCs cells after being cocultured for 48 h. Testosterone production significantly increased in response to low concentrations of LH (0–0.1 IU/mL) in the cocultured model but no increase was observed at high concentration (1 IU/mL). (c) Effect of GCs on androstenedione production responded to LH in TCs cells after being cocultured for 48 h. Androstenedione production significantly increased in response to different concentrations of LH (0-1 IU/mL) in the cocultured model. Data were expressed as mean ± SEM of three independent experiments. The asterisk showed the statistically significant difference.   ∗P < 0.05.
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fig4: (a) Effect of granulosa cells on mRNA expression of LHR in TCs cells and LH stimulation of androgen production in TCs in the cocultured model. The LHR mRNA expression of TCs in cocultured model increased significantly at 48 h but manifested no difference compared with TCs cultured alone at 24 h and 72 h (GAPDH as internal control). (b) Effect of GCs on testosterone production responded to LH in TCs cells after being cocultured for 48 h. Testosterone production significantly increased in response to low concentrations of LH (0–0.1 IU/mL) in the cocultured model but no increase was observed at high concentration (1 IU/mL). (c) Effect of GCs on androstenedione production responded to LH in TCs cells after being cocultured for 48 h. Androstenedione production significantly increased in response to different concentrations of LH (0-1 IU/mL) in the cocultured model. Data were expressed as mean ± SEM of three independent experiments. The asterisk showed the statistically significant difference.   ∗P < 0.05.
Mentions: While the LHR mRNA expression of TCs in the cocultured model manifested no difference compared with TCs cultured alone at 24 h (P > 0.05, Figure 4(a)), at 48 h the LHR mRNA expression of TCs increased significantly (P < 0.05, Figure 4(a)), indicating that paracrine factors secreted from GC cells could stimulate the LHR mRNA expression in TCs. The subsequent disappearance of difference between TCs cultured alone and cocultured model may reflect the suboptimal conditions related to in vitro environment. The cocultured GCs could enhance the capacity of LH responsiveness through increasing LHR mRNA expression in TCs.

Bottom Line: Interaction partners of follicular cells play a significant role in steroidogenesis, follicular formation, and development.Thus, GCs were capable of promoting steroidogenesis and LH responsiveness in TCs.This study provided a basis for further exploration of ovarian endocrine mechanism and pathologies.

View Article: PubMed Central - PubMed

Affiliation: Clinical Center of Reproductive Medicine, Affiliated Hospital of Weifang Medical University, Weifang, Shandong 261031, China.

ABSTRACT
Interaction partners of follicular cells play a significant role in steroidogenesis, follicular formation, and development. Androgen secreted by theca cells (TCs) can initiate follicle development and ovulation and provide precursor materials for estrogen synthesis. Therefore, studies on ovarian microenvironment will not only lead to better understanding of the steroidogenesis but also have clinical significance for ovarian endocrine abnormalities such as hyperandrogenism in polycystic ovary syndrome (PCOS). This study applied the Transwell coculture model to investigate if the interaction between granulosa and theca cells may affect androgen production in theca cells. Concentrations of testosterone and androstenedione in the spent medium were measured by radioimmunoassay and enzyme linked immunosorbent assay, respectively. The results show that the coculture with granulosa cells (GCs) increases steroidogenesis in TCs. In addition, testosterone and androstenedione productions in response to LH stimulation were also increased in the coculture model. Significantly increased mRNA expressions of steroidogenic enzymes (Star, Cyp11a1, Cyp17a1, and Hsd3b2) were observed in the cocultured TCs. Thus, GCs were capable of promoting steroidogenesis and LH responsiveness in TCs. This study provided a basis for further exploration of ovarian endocrine mechanism and pathologies.

No MeSH data available.


Related in: MedlinePlus