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Paracrine Regulation of Steroidogenesis in Theca Cells by Granulosa Cells Derived from Mouse Preantral Follicles.

Liu X, Qiao P, Jiang A, Jiang J, Han H, Wang L, Ren C - Biomed Res Int (2015)

Bottom Line: Interaction partners of follicular cells play a significant role in steroidogenesis, follicular formation, and development.Thus, GCs were capable of promoting steroidogenesis and LH responsiveness in TCs.This study provided a basis for further exploration of ovarian endocrine mechanism and pathologies.

View Article: PubMed Central - PubMed

Affiliation: Clinical Center of Reproductive Medicine, Affiliated Hospital of Weifang Medical University, Weifang, Shandong 261031, China.

ABSTRACT
Interaction partners of follicular cells play a significant role in steroidogenesis, follicular formation, and development. Androgen secreted by theca cells (TCs) can initiate follicle development and ovulation and provide precursor materials for estrogen synthesis. Therefore, studies on ovarian microenvironment will not only lead to better understanding of the steroidogenesis but also have clinical significance for ovarian endocrine abnormalities such as hyperandrogenism in polycystic ovary syndrome (PCOS). This study applied the Transwell coculture model to investigate if the interaction between granulosa and theca cells may affect androgen production in theca cells. Concentrations of testosterone and androstenedione in the spent medium were measured by radioimmunoassay and enzyme linked immunosorbent assay, respectively. The results show that the coculture with granulosa cells (GCs) increases steroidogenesis in TCs. In addition, testosterone and androstenedione productions in response to LH stimulation were also increased in the coculture model. Significantly increased mRNA expressions of steroidogenic enzymes (Star, Cyp11a1, Cyp17a1, and Hsd3b2) were observed in the cocultured TCs. Thus, GCs were capable of promoting steroidogenesis and LH responsiveness in TCs. This study provided a basis for further exploration of ovarian endocrine mechanism and pathologies.

No MeSH data available.


Related in: MedlinePlus

Effect of granulosa cells on mRNA expression of genes encoding steroidogenic enzymes (Star, Cyp11a1, Cyp17a1, and Hsd3b2) in TCs. After TCs were cultured up to 72 h, the expressions of Star, Cyp11a1, Cyp17a1, and Hsd3b2 were measured by quantitative real-time PCR (GAPDH as internal control). Expressions of all these genes were increased in the cocultured model compared with TCs cultured alone. Data were the mean ± SEM of three independent experiments where all the samples were repeated in thrice. The asterisk showed the statistically significant difference.   ∗P < 0.05.
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fig3: Effect of granulosa cells on mRNA expression of genes encoding steroidogenic enzymes (Star, Cyp11a1, Cyp17a1, and Hsd3b2) in TCs. After TCs were cultured up to 72 h, the expressions of Star, Cyp11a1, Cyp17a1, and Hsd3b2 were measured by quantitative real-time PCR (GAPDH as internal control). Expressions of all these genes were increased in the cocultured model compared with TCs cultured alone. Data were the mean ± SEM of three independent experiments where all the samples were repeated in thrice. The asterisk showed the statistically significant difference.   ∗P < 0.05.

Mentions: To investigate the regulatory pathway leading to the increased androgen production, quantitative real-time PCR was performed to determine the expression of genes involved in androgen synthesis Star, Cyp11a1, Cyp17a1, and Hsd3b2 at 24 h, 48 h, and 72 h. As shown in Figure 3, the results revealed that the expressions of all these genes were increased in the cocultured model compared with TCs cultured alone (P < 0.05). Thus, increasing expression of the genes involved with androgen synthesis appeared to account for the increased androgen production. These results indicated that paracrine factors secreted from GCs can pass the Transwell insert membrane and regulate the transcription of steroidogenic enzymes.


Paracrine Regulation of Steroidogenesis in Theca Cells by Granulosa Cells Derived from Mouse Preantral Follicles.

Liu X, Qiao P, Jiang A, Jiang J, Han H, Wang L, Ren C - Biomed Res Int (2015)

Effect of granulosa cells on mRNA expression of genes encoding steroidogenic enzymes (Star, Cyp11a1, Cyp17a1, and Hsd3b2) in TCs. After TCs were cultured up to 72 h, the expressions of Star, Cyp11a1, Cyp17a1, and Hsd3b2 were measured by quantitative real-time PCR (GAPDH as internal control). Expressions of all these genes were increased in the cocultured model compared with TCs cultured alone. Data were the mean ± SEM of three independent experiments where all the samples were repeated in thrice. The asterisk showed the statistically significant difference.   ∗P < 0.05.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4556819&req=5

fig3: Effect of granulosa cells on mRNA expression of genes encoding steroidogenic enzymes (Star, Cyp11a1, Cyp17a1, and Hsd3b2) in TCs. After TCs were cultured up to 72 h, the expressions of Star, Cyp11a1, Cyp17a1, and Hsd3b2 were measured by quantitative real-time PCR (GAPDH as internal control). Expressions of all these genes were increased in the cocultured model compared with TCs cultured alone. Data were the mean ± SEM of three independent experiments where all the samples were repeated in thrice. The asterisk showed the statistically significant difference.   ∗P < 0.05.
Mentions: To investigate the regulatory pathway leading to the increased androgen production, quantitative real-time PCR was performed to determine the expression of genes involved in androgen synthesis Star, Cyp11a1, Cyp17a1, and Hsd3b2 at 24 h, 48 h, and 72 h. As shown in Figure 3, the results revealed that the expressions of all these genes were increased in the cocultured model compared with TCs cultured alone (P < 0.05). Thus, increasing expression of the genes involved with androgen synthesis appeared to account for the increased androgen production. These results indicated that paracrine factors secreted from GCs can pass the Transwell insert membrane and regulate the transcription of steroidogenic enzymes.

Bottom Line: Interaction partners of follicular cells play a significant role in steroidogenesis, follicular formation, and development.Thus, GCs were capable of promoting steroidogenesis and LH responsiveness in TCs.This study provided a basis for further exploration of ovarian endocrine mechanism and pathologies.

View Article: PubMed Central - PubMed

Affiliation: Clinical Center of Reproductive Medicine, Affiliated Hospital of Weifang Medical University, Weifang, Shandong 261031, China.

ABSTRACT
Interaction partners of follicular cells play a significant role in steroidogenesis, follicular formation, and development. Androgen secreted by theca cells (TCs) can initiate follicle development and ovulation and provide precursor materials for estrogen synthesis. Therefore, studies on ovarian microenvironment will not only lead to better understanding of the steroidogenesis but also have clinical significance for ovarian endocrine abnormalities such as hyperandrogenism in polycystic ovary syndrome (PCOS). This study applied the Transwell coculture model to investigate if the interaction between granulosa and theca cells may affect androgen production in theca cells. Concentrations of testosterone and androstenedione in the spent medium were measured by radioimmunoassay and enzyme linked immunosorbent assay, respectively. The results show that the coculture with granulosa cells (GCs) increases steroidogenesis in TCs. In addition, testosterone and androstenedione productions in response to LH stimulation were also increased in the coculture model. Significantly increased mRNA expressions of steroidogenic enzymes (Star, Cyp11a1, Cyp17a1, and Hsd3b2) were observed in the cocultured TCs. Thus, GCs were capable of promoting steroidogenesis and LH responsiveness in TCs. This study provided a basis for further exploration of ovarian endocrine mechanism and pathologies.

No MeSH data available.


Related in: MedlinePlus