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Paracrine Regulation of Steroidogenesis in Theca Cells by Granulosa Cells Derived from Mouse Preantral Follicles.

Liu X, Qiao P, Jiang A, Jiang J, Han H, Wang L, Ren C - Biomed Res Int (2015)

Bottom Line: Interaction partners of follicular cells play a significant role in steroidogenesis, follicular formation, and development.Thus, GCs were capable of promoting steroidogenesis and LH responsiveness in TCs.This study provided a basis for further exploration of ovarian endocrine mechanism and pathologies.

View Article: PubMed Central - PubMed

Affiliation: Clinical Center of Reproductive Medicine, Affiliated Hospital of Weifang Medical University, Weifang, Shandong 261031, China.

ABSTRACT
Interaction partners of follicular cells play a significant role in steroidogenesis, follicular formation, and development. Androgen secreted by theca cells (TCs) can initiate follicle development and ovulation and provide precursor materials for estrogen synthesis. Therefore, studies on ovarian microenvironment will not only lead to better understanding of the steroidogenesis but also have clinical significance for ovarian endocrine abnormalities such as hyperandrogenism in polycystic ovary syndrome (PCOS). This study applied the Transwell coculture model to investigate if the interaction between granulosa and theca cells may affect androgen production in theca cells. Concentrations of testosterone and androstenedione in the spent medium were measured by radioimmunoassay and enzyme linked immunosorbent assay, respectively. The results show that the coculture with granulosa cells (GCs) increases steroidogenesis in TCs. In addition, testosterone and androstenedione productions in response to LH stimulation were also increased in the coculture model. Significantly increased mRNA expressions of steroidogenic enzymes (Star, Cyp11a1, Cyp17a1, and Hsd3b2) were observed in the cocultured TCs. Thus, GCs were capable of promoting steroidogenesis and LH responsiveness in TCs. This study provided a basis for further exploration of ovarian endocrine mechanism and pathologies.

No MeSH data available.


Related in: MedlinePlus

Effect of GCs on steroidogenesis in TCs. Concentrations of testosterone and androstenedione in the spent medium were measured by radioimmunoassay and enzyme linked immunosorbent assay, respectively. (a) and (c) An increased amount of testosterone and androstenedione was observed in cocultured model. (b) and (d) GCs produced diminished levels of testosterone and androstenedione, respectively. Note the scale difference between (a) and (c) as well as between (b) and (d). Data were expressed as mean ± SEM of three independent experiments. The asterisk marked the statistically significant difference.
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fig2: Effect of GCs on steroidogenesis in TCs. Concentrations of testosterone and androstenedione in the spent medium were measured by radioimmunoassay and enzyme linked immunosorbent assay, respectively. (a) and (c) An increased amount of testosterone and androstenedione was observed in cocultured model. (b) and (d) GCs produced diminished levels of testosterone and androstenedione, respectively. Note the scale difference between (a) and (c) as well as between (b) and (d). Data were expressed as mean ± SEM of three independent experiments. The asterisk marked the statistically significant difference.

Mentions: Concentrations of testosterone and androstenedione in the spent medium were measured at 24 h, 48 h, and 72 h using RIA and ELISA, respectively. When cocultured with GCs, TCs produced an increased amount of testosterone and androstenedione than TCs cultured alone (P < 0.05, Figure 2(a) for testosterone and Figure 2(c) for androstenedione). Interestingly, when TCs were cultured alone, the production of testosterone and androstenedione showed a declining trend along time, which may reflect the adaptation change to in vitro conditions lacking the supports from other cell types. In the spent medium of GCs cultured alone, the levels of testosterone (Figure 2(b)) and androstenedione (Figure 2(d)) were both diminished in comparison with TCs. And no alterations were observed following different time of in vitro culture (P > 0.05). Taken together, these results indicated that GCs could promote TCs production of testosterone and androstenedione.


Paracrine Regulation of Steroidogenesis in Theca Cells by Granulosa Cells Derived from Mouse Preantral Follicles.

Liu X, Qiao P, Jiang A, Jiang J, Han H, Wang L, Ren C - Biomed Res Int (2015)

Effect of GCs on steroidogenesis in TCs. Concentrations of testosterone and androstenedione in the spent medium were measured by radioimmunoassay and enzyme linked immunosorbent assay, respectively. (a) and (c) An increased amount of testosterone and androstenedione was observed in cocultured model. (b) and (d) GCs produced diminished levels of testosterone and androstenedione, respectively. Note the scale difference between (a) and (c) as well as between (b) and (d). Data were expressed as mean ± SEM of three independent experiments. The asterisk marked the statistically significant difference.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4556819&req=5

fig2: Effect of GCs on steroidogenesis in TCs. Concentrations of testosterone and androstenedione in the spent medium were measured by radioimmunoassay and enzyme linked immunosorbent assay, respectively. (a) and (c) An increased amount of testosterone and androstenedione was observed in cocultured model. (b) and (d) GCs produced diminished levels of testosterone and androstenedione, respectively. Note the scale difference between (a) and (c) as well as between (b) and (d). Data were expressed as mean ± SEM of three independent experiments. The asterisk marked the statistically significant difference.
Mentions: Concentrations of testosterone and androstenedione in the spent medium were measured at 24 h, 48 h, and 72 h using RIA and ELISA, respectively. When cocultured with GCs, TCs produced an increased amount of testosterone and androstenedione than TCs cultured alone (P < 0.05, Figure 2(a) for testosterone and Figure 2(c) for androstenedione). Interestingly, when TCs were cultured alone, the production of testosterone and androstenedione showed a declining trend along time, which may reflect the adaptation change to in vitro conditions lacking the supports from other cell types. In the spent medium of GCs cultured alone, the levels of testosterone (Figure 2(b)) and androstenedione (Figure 2(d)) were both diminished in comparison with TCs. And no alterations were observed following different time of in vitro culture (P > 0.05). Taken together, these results indicated that GCs could promote TCs production of testosterone and androstenedione.

Bottom Line: Interaction partners of follicular cells play a significant role in steroidogenesis, follicular formation, and development.Thus, GCs were capable of promoting steroidogenesis and LH responsiveness in TCs.This study provided a basis for further exploration of ovarian endocrine mechanism and pathologies.

View Article: PubMed Central - PubMed

Affiliation: Clinical Center of Reproductive Medicine, Affiliated Hospital of Weifang Medical University, Weifang, Shandong 261031, China.

ABSTRACT
Interaction partners of follicular cells play a significant role in steroidogenesis, follicular formation, and development. Androgen secreted by theca cells (TCs) can initiate follicle development and ovulation and provide precursor materials for estrogen synthesis. Therefore, studies on ovarian microenvironment will not only lead to better understanding of the steroidogenesis but also have clinical significance for ovarian endocrine abnormalities such as hyperandrogenism in polycystic ovary syndrome (PCOS). This study applied the Transwell coculture model to investigate if the interaction between granulosa and theca cells may affect androgen production in theca cells. Concentrations of testosterone and androstenedione in the spent medium were measured by radioimmunoassay and enzyme linked immunosorbent assay, respectively. The results show that the coculture with granulosa cells (GCs) increases steroidogenesis in TCs. In addition, testosterone and androstenedione productions in response to LH stimulation were also increased in the coculture model. Significantly increased mRNA expressions of steroidogenic enzymes (Star, Cyp11a1, Cyp17a1, and Hsd3b2) were observed in the cocultured TCs. Thus, GCs were capable of promoting steroidogenesis and LH responsiveness in TCs. This study provided a basis for further exploration of ovarian endocrine mechanism and pathologies.

No MeSH data available.


Related in: MedlinePlus