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Association between pro-(IL-8) and anti-inflammatory (IL-10) cytokine variants and their serum levels and H. pylori-related gastric carcinogenesis in northern India.

Kumar S, Kumari N, Mittal RD, Mohindra S, Ghoshal UC - Meta Gene (2015)

Bottom Line: IL-8/IL-10 ratio was lower among GC than HC but higher than FD (3.7 [0.18-38.41] vs. 6.59 [0.98-130.2], p < 0.001 and 4.22 [0.15-61.4], p < 0.01).Circulating levels of IL-8, IL-10 and IL-8/lL-10 ratios were different among H. pylori-infected and non-infected GC than HC (p < 0.001, p < 0.001 and p < 0.01).Pro-(IL-8)-251 T/A and anti-inflammatory (IL-10)-819 C/T gene polymorphisms and their circulating levels may play a role in H. pylori-associated gastric carcinogenesis in northern India.

View Article: PubMed Central - PubMed

Affiliation: Department of Gastroenterology, Sanjay Gandhi Postgraduate Institute of Medical Sciences, Lucknow 226014, India.

ABSTRACT

Background: Interleukin (IL)-8 -251 T/A and IL-10 (-1082 G/A and -819/592 C/T) polymorphisms and their expression may influence gastritis, atrophy, intestinal metaplasia (IM) and gastric cancer (GC) following H. pylori infection.

Methods: Genotyping of these genes was performed (ASO-PCR) in 200, 182 and 250 with GC, functional dyspepsia (FD) and healthy controls (HC), respectively. Anti-H. pylori IgG-antibody was tested in all and serums IL-8 and IL-10 were measured randomly in 60 subjects of each group by ELISA.

Results: Pro-(IL-8)-251 AA and anti-inflammatory (IL-10)-819 TT genotypes were commoner among GC than HC (p = 0.023, OR 1.86 [1.09-3.2] and p = 0.020, OR 2.0 [1.11-3.5]) but comparable with FD. IL-8 AA and IL-10-819 T allele carriage was also commoner in H. pylori-infected GC than HC (p = 0.011, OR 2.47 [1.23-5.0], and p = 0.018, OR 2.3 (1.16-4.59). IL-10-1082 G/A genotype and haplotypes (ACC, GCC, ATA and GTA) were comparable in all groups. Circulating levels of IL-8 and IL-10 were higher among GC than HC but comparable to FD (IL-8; 57.64 [6.44-319.46] vs. 54.35 [4.24-318.96] and 26.33 [4.67-304.54] pg/ml, p < 0.001 and IL-10; 15.47 [1.01-270.87] vs. 12.28 [0.96-64.88] and 3.79 [1.24-56.65], p < 0.001 for GC vs. HC). IL-8/IL-10 ratio was lower among GC than HC but higher than FD (3.7 [0.18-38.41] vs. 6.59 [0.98-130.2], p < 0.001 and 4.22 [0.15-61.4], p < 0.01). Circulating levels of IL-8, IL-10 and IL-8/lL-10 ratios were different among H. pylori-infected and non-infected GC than HC (p < 0.001, p < 0.001 and p < 0.01).

Conclusions: Pro-(IL-8)-251 T/A and anti-inflammatory (IL-10)-819 C/T gene polymorphisms and their circulating levels may play a role in H. pylori-associated gastric carcinogenesis in northern India.

No MeSH data available.


Related in: MedlinePlus

Frequency of IL-10-1082 G/A and -819 (C/T)/592 (C/A) haplotypes among cases and controls. GC = gastric cancer, FD = functional dyspepsia, HC = healthy control and vs. = versus; *Haplotype number (n) represents total number of chromosomes; frequency of combination equivalent to zero in any cell for IL-10-1082 or IL-10-819/592 was not included in analysis; frequency of haplotypes was comparable among case and controls.
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f0005: Frequency of IL-10-1082 G/A and -819 (C/T)/592 (C/A) haplotypes among cases and controls. GC = gastric cancer, FD = functional dyspepsia, HC = healthy control and vs. = versus; *Haplotype number (n) represents total number of chromosomes; frequency of combination equivalent to zero in any cell for IL-10-1082 or IL-10-819/592 was not included in analysis; frequency of haplotypes was comparable among case and controls.

Mentions:  Haplotypes were evaluated for IL-10-1082 G/A, -819 C/T and -592 C/A (-819 and -592 are in linkage disequilibrium) polymorphisms located in the same gene with emphasis on combination of variants, which might be more likely to influence change in IL-10 level. Four haplotypes, over-producers (GCC and GTA), intermediate (ACC) and under-producer (ATA) were constructed. Frequency of haplotypes was comparable among cases and controls as shown in Fig. 1.


Association between pro-(IL-8) and anti-inflammatory (IL-10) cytokine variants and their serum levels and H. pylori-related gastric carcinogenesis in northern India.

Kumar S, Kumari N, Mittal RD, Mohindra S, Ghoshal UC - Meta Gene (2015)

Frequency of IL-10-1082 G/A and -819 (C/T)/592 (C/A) haplotypes among cases and controls. GC = gastric cancer, FD = functional dyspepsia, HC = healthy control and vs. = versus; *Haplotype number (n) represents total number of chromosomes; frequency of combination equivalent to zero in any cell for IL-10-1082 or IL-10-819/592 was not included in analysis; frequency of haplotypes was comparable among case and controls.
© Copyright Policy - CC BY-NC-ND
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4556814&req=5

f0005: Frequency of IL-10-1082 G/A and -819 (C/T)/592 (C/A) haplotypes among cases and controls. GC = gastric cancer, FD = functional dyspepsia, HC = healthy control and vs. = versus; *Haplotype number (n) represents total number of chromosomes; frequency of combination equivalent to zero in any cell for IL-10-1082 or IL-10-819/592 was not included in analysis; frequency of haplotypes was comparable among case and controls.
Mentions:  Haplotypes were evaluated for IL-10-1082 G/A, -819 C/T and -592 C/A (-819 and -592 are in linkage disequilibrium) polymorphisms located in the same gene with emphasis on combination of variants, which might be more likely to influence change in IL-10 level. Four haplotypes, over-producers (GCC and GTA), intermediate (ACC) and under-producer (ATA) were constructed. Frequency of haplotypes was comparable among cases and controls as shown in Fig. 1.

Bottom Line: IL-8/IL-10 ratio was lower among GC than HC but higher than FD (3.7 [0.18-38.41] vs. 6.59 [0.98-130.2], p < 0.001 and 4.22 [0.15-61.4], p < 0.01).Circulating levels of IL-8, IL-10 and IL-8/lL-10 ratios were different among H. pylori-infected and non-infected GC than HC (p < 0.001, p < 0.001 and p < 0.01).Pro-(IL-8)-251 T/A and anti-inflammatory (IL-10)-819 C/T gene polymorphisms and their circulating levels may play a role in H. pylori-associated gastric carcinogenesis in northern India.

View Article: PubMed Central - PubMed

Affiliation: Department of Gastroenterology, Sanjay Gandhi Postgraduate Institute of Medical Sciences, Lucknow 226014, India.

ABSTRACT

Background: Interleukin (IL)-8 -251 T/A and IL-10 (-1082 G/A and -819/592 C/T) polymorphisms and their expression may influence gastritis, atrophy, intestinal metaplasia (IM) and gastric cancer (GC) following H. pylori infection.

Methods: Genotyping of these genes was performed (ASO-PCR) in 200, 182 and 250 with GC, functional dyspepsia (FD) and healthy controls (HC), respectively. Anti-H. pylori IgG-antibody was tested in all and serums IL-8 and IL-10 were measured randomly in 60 subjects of each group by ELISA.

Results: Pro-(IL-8)-251 AA and anti-inflammatory (IL-10)-819 TT genotypes were commoner among GC than HC (p = 0.023, OR 1.86 [1.09-3.2] and p = 0.020, OR 2.0 [1.11-3.5]) but comparable with FD. IL-8 AA and IL-10-819 T allele carriage was also commoner in H. pylori-infected GC than HC (p = 0.011, OR 2.47 [1.23-5.0], and p = 0.018, OR 2.3 (1.16-4.59). IL-10-1082 G/A genotype and haplotypes (ACC, GCC, ATA and GTA) were comparable in all groups. Circulating levels of IL-8 and IL-10 were higher among GC than HC but comparable to FD (IL-8; 57.64 [6.44-319.46] vs. 54.35 [4.24-318.96] and 26.33 [4.67-304.54] pg/ml, p < 0.001 and IL-10; 15.47 [1.01-270.87] vs. 12.28 [0.96-64.88] and 3.79 [1.24-56.65], p < 0.001 for GC vs. HC). IL-8/IL-10 ratio was lower among GC than HC but higher than FD (3.7 [0.18-38.41] vs. 6.59 [0.98-130.2], p < 0.001 and 4.22 [0.15-61.4], p < 0.01). Circulating levels of IL-8, IL-10 and IL-8/lL-10 ratios were different among H. pylori-infected and non-infected GC than HC (p < 0.001, p < 0.001 and p < 0.01).

Conclusions: Pro-(IL-8)-251 T/A and anti-inflammatory (IL-10)-819 C/T gene polymorphisms and their circulating levels may play a role in H. pylori-associated gastric carcinogenesis in northern India.

No MeSH data available.


Related in: MedlinePlus