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Function of the CRISPR-Cas System of the Human Pathogen Clostridium difficile.

Boudry P, Semenova E, Monot M, Datsenko KA, Lopatina A, Sekulovic O, Ospina-Bedoya M, Fortier LC, Severinov K, Dupuy B, Soutourina O - MBio (2015)

Bottom Line: Clostridium difficile is the major cause of nosocomial infections associated with antibiotic therapy worldwide.We provide experimental evidence for the function of the C. difficile CRISPR system against plasmid DNA and bacteriophages.These data demonstrate the original features of active C. difficile CRISPR system and bring important insights into the interactions of this major enteropathogen with foreign DNA invaders during its infection cycle.

View Article: PubMed Central - PubMed

Affiliation: Laboratoire Pathogenèse des Bactéries Anaérobies, Institut Pasteur, Paris, France Université Paris Diderot, Sorbonne Paris Cité, Cellule Pasteur, Paris, France.

No MeSH data available.


Related in: MedlinePlus

Spacer homology analysis of CRISPR arrays for nine C. difficile strains. The spacer content of each CRISPR array is shown. The names of CRISPR arrays transcribed on the plus or minus strand are shown on green and red, respectively. The same number was assigned to identical spacers within CRISPR arrays from different strains. Color was used to show the spacer matching the clostridial phage genome sequence (red), plasmid (dark green), chromosomal prophage region (yellow), other chromosomal region (light green), chromosome and phage or plasmid (taupe), both phage and plasmid (blue), phage and prophage (mauve), and three groups (chromosome, plasmid, and phage0 (gray). Potential spacer deletion events are shown in bold type.
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fig4: Spacer homology analysis of CRISPR arrays for nine C. difficile strains. The spacer content of each CRISPR array is shown. The names of CRISPR arrays transcribed on the plus or minus strand are shown on green and red, respectively. The same number was assigned to identical spacers within CRISPR arrays from different strains. Color was used to show the spacer matching the clostridial phage genome sequence (red), plasmid (dark green), chromosomal prophage region (yellow), other chromosomal region (light green), chromosome and phage or plasmid (taupe), both phage and plasmid (blue), phage and prophage (mauve), and three groups (chromosome, plasmid, and phage0 (gray). Potential spacer deletion events are shown in bold type.

Mentions: More than one-third of all analyzed spacers targeted the Clostridium phages, and about half of the hits to the Clostridium chromosome corresponded to the prophage regions (Fig. 4; see Table S2A in the supplemental material). For example, among 66 spacers from strain CF5, 29 matched chromosome sequences of which 17 were from prophages. These observations suggest that all the C. difficile strains analyzed had intensive interactions with phages. We also detected hits to Clostridium plasmids especially for CRISPR spacers from strain M120. Interestingly, this strain of PCR ribotype 078 possesses the largest number of unique spacers (153 spacers) that extensively target foreign DNA elements and are distributed in the six CRISPR arrays (Fig. 4, Fig. S3, and Table S2A).


Function of the CRISPR-Cas System of the Human Pathogen Clostridium difficile.

Boudry P, Semenova E, Monot M, Datsenko KA, Lopatina A, Sekulovic O, Ospina-Bedoya M, Fortier LC, Severinov K, Dupuy B, Soutourina O - MBio (2015)

Spacer homology analysis of CRISPR arrays for nine C. difficile strains. The spacer content of each CRISPR array is shown. The names of CRISPR arrays transcribed on the plus or minus strand are shown on green and red, respectively. The same number was assigned to identical spacers within CRISPR arrays from different strains. Color was used to show the spacer matching the clostridial phage genome sequence (red), plasmid (dark green), chromosomal prophage region (yellow), other chromosomal region (light green), chromosome and phage or plasmid (taupe), both phage and plasmid (blue), phage and prophage (mauve), and three groups (chromosome, plasmid, and phage0 (gray). Potential spacer deletion events are shown in bold type.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4556805&req=5

fig4: Spacer homology analysis of CRISPR arrays for nine C. difficile strains. The spacer content of each CRISPR array is shown. The names of CRISPR arrays transcribed on the plus or minus strand are shown on green and red, respectively. The same number was assigned to identical spacers within CRISPR arrays from different strains. Color was used to show the spacer matching the clostridial phage genome sequence (red), plasmid (dark green), chromosomal prophage region (yellow), other chromosomal region (light green), chromosome and phage or plasmid (taupe), both phage and plasmid (blue), phage and prophage (mauve), and three groups (chromosome, plasmid, and phage0 (gray). Potential spacer deletion events are shown in bold type.
Mentions: More than one-third of all analyzed spacers targeted the Clostridium phages, and about half of the hits to the Clostridium chromosome corresponded to the prophage regions (Fig. 4; see Table S2A in the supplemental material). For example, among 66 spacers from strain CF5, 29 matched chromosome sequences of which 17 were from prophages. These observations suggest that all the C. difficile strains analyzed had intensive interactions with phages. We also detected hits to Clostridium plasmids especially for CRISPR spacers from strain M120. Interestingly, this strain of PCR ribotype 078 possesses the largest number of unique spacers (153 spacers) that extensively target foreign DNA elements and are distributed in the six CRISPR arrays (Fig. 4, Fig. S3, and Table S2A).

Bottom Line: Clostridium difficile is the major cause of nosocomial infections associated with antibiotic therapy worldwide.We provide experimental evidence for the function of the C. difficile CRISPR system against plasmid DNA and bacteriophages.These data demonstrate the original features of active C. difficile CRISPR system and bring important insights into the interactions of this major enteropathogen with foreign DNA invaders during its infection cycle.

View Article: PubMed Central - PubMed

Affiliation: Laboratoire Pathogenèse des Bactéries Anaérobies, Institut Pasteur, Paris, France Université Paris Diderot, Sorbonne Paris Cité, Cellule Pasteur, Paris, France.

No MeSH data available.


Related in: MedlinePlus