Limits...
Topical application of herbal formula for the treatment of ligature-induced periodontitis.

Kim MH, Choi YY, Lee HJ, Lee H, Park JC, Yang WM - J Periodontal Implant Sci (2015)

Bottom Line: Alveolar bone resorption was significantly reduced in the PerioH-035-treated groups.In addition, periodontal tissue destruction, especially cementum demineralization, was ameliorated in the P1 and P100 groups.Moreover, gingival tissue from the PerioH-035-treated group showed a decrease in the MMP-9 mRNA level, resulting in recovery of collagen degradation.

View Article: PubMed Central - PubMed

Affiliation: Department of Convergence Korean Medical Science, Kyung Hee University College of Korean Medicine, Seoul, Korea.

ABSTRACT

Purpose: The aim of this study was to investigate the therapeutic effects of a herbal formula, PerioH-035, containing Angelica sinensis, steamed Rehmannia glutinosa, Angelica dahurica, Cimicifuga heracleifolia, and Zanthoxylum piperitum on the periodontal breakdown in a well-established ligature-induced periodontitis model in rats.

Methods: Sprague-Dawley rats were randomly assigned to 1 of 4 groups: NL (non-ligatured), L (ligatured), P1 (ligatured and treated with 1 mg/mL PerioH-035), P100 (ligatured and treated with 100 mg/mL PerioH-035). Periodontitis was induced by placing a ligature around the mandibular first molars. PerioH-035 was topically applied to both sides of the first molar for 2 weeks. The right side of the mandibles was retrieved for micro-computed tomography (CT) and methylene blue staining to analyze alveolar bone loss. The left side of the mandibles was histologically analyzed by TRAP and H&E staining. The MMP-9 mRNA level in gingival tissue was investigated by RT-PCR.

Results: Alveolar bone resorption was significantly reduced in the PerioH-035-treated groups. The number of dense multi-nucleated cells found to be TRAP-positive by staining in the ligatured rats was markedly decreased by PerioH-035 application. In addition, periodontal tissue destruction, especially cementum demineralization, was ameliorated in the P1 and P100 groups. Moreover, gingival tissue from the PerioH-035-treated group showed a decrease in the MMP-9 mRNA level, resulting in recovery of collagen degradation.

Conclusions: These results suggest that PerioH-035 has therapeutic effects on periodontitis, and thus, PerioH-035 shows promise as a treatment for periodontitis.

No MeSH data available.


Related in: MedlinePlus

The effect of PerioH-035 on alveolar bone loss indicated by methylene blue staining. PerioH-35 is an herbal formula, containing Angelica sinensis, steamed Rehmannia glutinosa, Angelica dahurica, Cimicifuga heracleifolia, and Zanthoxylum piperitum. (A) Representative images demonstrate the alveolar bone resorption. The scale bar is 1 mm. (B) The measurement of vertical alveolar bone resorption is illustrated, and the application of P100 has significantly inhibited the alveolar bone resorption. The red lines represent the distance from the cementoenamel junction to the alveolar bone crest. Data shown are mean ±S.E.M (NL = 2,720±130 µm; L = 3,610±280 µm; P1 = 3,003±140 µm; P100 = 2,920±310 µm). Mean values were significantly different among groups. (a)P<0.05, b)P<0.01). NL, non-ligatured; L, ligatured; P1, ligatured and treated with 1 mg/mL PerioH-035; P100, ligatured and treated with 100 mg/mL PerioH-035.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4556800&req=5

Figure 2: The effect of PerioH-035 on alveolar bone loss indicated by methylene blue staining. PerioH-35 is an herbal formula, containing Angelica sinensis, steamed Rehmannia glutinosa, Angelica dahurica, Cimicifuga heracleifolia, and Zanthoxylum piperitum. (A) Representative images demonstrate the alveolar bone resorption. The scale bar is 1 mm. (B) The measurement of vertical alveolar bone resorption is illustrated, and the application of P100 has significantly inhibited the alveolar bone resorption. The red lines represent the distance from the cementoenamel junction to the alveolar bone crest. Data shown are mean ±S.E.M (NL = 2,720±130 µm; L = 3,610±280 µm; P1 = 3,003±140 µm; P100 = 2,920±310 µm). Mean values were significantly different among groups. (a)P<0.05, b)P<0.01). NL, non-ligatured; L, ligatured; P1, ligatured and treated with 1 mg/mL PerioH-035; P100, ligatured and treated with 100 mg/mL PerioH-035.

Mentions: Similar results were shown in the methylene blue staining. The rats with ligatures showed significant ABL—about 1.32 times that of NL (NL = 2,720±130 µm; L = 3,610±280 µm; P<0.001). It was observed that treatment with PerioH-035 1 and 100 mg/mL (3,003±140 and 2,920±310 µm; 16.81% and 19.11%, respectively) significantly reversed the ABL caused by ligature-induced periodontitis (Fig. 2).


Topical application of herbal formula for the treatment of ligature-induced periodontitis.

Kim MH, Choi YY, Lee HJ, Lee H, Park JC, Yang WM - J Periodontal Implant Sci (2015)

The effect of PerioH-035 on alveolar bone loss indicated by methylene blue staining. PerioH-35 is an herbal formula, containing Angelica sinensis, steamed Rehmannia glutinosa, Angelica dahurica, Cimicifuga heracleifolia, and Zanthoxylum piperitum. (A) Representative images demonstrate the alveolar bone resorption. The scale bar is 1 mm. (B) The measurement of vertical alveolar bone resorption is illustrated, and the application of P100 has significantly inhibited the alveolar bone resorption. The red lines represent the distance from the cementoenamel junction to the alveolar bone crest. Data shown are mean ±S.E.M (NL = 2,720±130 µm; L = 3,610±280 µm; P1 = 3,003±140 µm; P100 = 2,920±310 µm). Mean values were significantly different among groups. (a)P<0.05, b)P<0.01). NL, non-ligatured; L, ligatured; P1, ligatured and treated with 1 mg/mL PerioH-035; P100, ligatured and treated with 100 mg/mL PerioH-035.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4556800&req=5

Figure 2: The effect of PerioH-035 on alveolar bone loss indicated by methylene blue staining. PerioH-35 is an herbal formula, containing Angelica sinensis, steamed Rehmannia glutinosa, Angelica dahurica, Cimicifuga heracleifolia, and Zanthoxylum piperitum. (A) Representative images demonstrate the alveolar bone resorption. The scale bar is 1 mm. (B) The measurement of vertical alveolar bone resorption is illustrated, and the application of P100 has significantly inhibited the alveolar bone resorption. The red lines represent the distance from the cementoenamel junction to the alveolar bone crest. Data shown are mean ±S.E.M (NL = 2,720±130 µm; L = 3,610±280 µm; P1 = 3,003±140 µm; P100 = 2,920±310 µm). Mean values were significantly different among groups. (a)P<0.05, b)P<0.01). NL, non-ligatured; L, ligatured; P1, ligatured and treated with 1 mg/mL PerioH-035; P100, ligatured and treated with 100 mg/mL PerioH-035.
Mentions: Similar results were shown in the methylene blue staining. The rats with ligatures showed significant ABL—about 1.32 times that of NL (NL = 2,720±130 µm; L = 3,610±280 µm; P<0.001). It was observed that treatment with PerioH-035 1 and 100 mg/mL (3,003±140 and 2,920±310 µm; 16.81% and 19.11%, respectively) significantly reversed the ABL caused by ligature-induced periodontitis (Fig. 2).

Bottom Line: Alveolar bone resorption was significantly reduced in the PerioH-035-treated groups.In addition, periodontal tissue destruction, especially cementum demineralization, was ameliorated in the P1 and P100 groups.Moreover, gingival tissue from the PerioH-035-treated group showed a decrease in the MMP-9 mRNA level, resulting in recovery of collagen degradation.

View Article: PubMed Central - PubMed

Affiliation: Department of Convergence Korean Medical Science, Kyung Hee University College of Korean Medicine, Seoul, Korea.

ABSTRACT

Purpose: The aim of this study was to investigate the therapeutic effects of a herbal formula, PerioH-035, containing Angelica sinensis, steamed Rehmannia glutinosa, Angelica dahurica, Cimicifuga heracleifolia, and Zanthoxylum piperitum on the periodontal breakdown in a well-established ligature-induced periodontitis model in rats.

Methods: Sprague-Dawley rats were randomly assigned to 1 of 4 groups: NL (non-ligatured), L (ligatured), P1 (ligatured and treated with 1 mg/mL PerioH-035), P100 (ligatured and treated with 100 mg/mL PerioH-035). Periodontitis was induced by placing a ligature around the mandibular first molars. PerioH-035 was topically applied to both sides of the first molar for 2 weeks. The right side of the mandibles was retrieved for micro-computed tomography (CT) and methylene blue staining to analyze alveolar bone loss. The left side of the mandibles was histologically analyzed by TRAP and H&E staining. The MMP-9 mRNA level in gingival tissue was investigated by RT-PCR.

Results: Alveolar bone resorption was significantly reduced in the PerioH-035-treated groups. The number of dense multi-nucleated cells found to be TRAP-positive by staining in the ligatured rats was markedly decreased by PerioH-035 application. In addition, periodontal tissue destruction, especially cementum demineralization, was ameliorated in the P1 and P100 groups. Moreover, gingival tissue from the PerioH-035-treated group showed a decrease in the MMP-9 mRNA level, resulting in recovery of collagen degradation.

Conclusions: These results suggest that PerioH-035 has therapeutic effects on periodontitis, and thus, PerioH-035 shows promise as a treatment for periodontitis.

No MeSH data available.


Related in: MedlinePlus