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Comparative analysis of carrier systems for delivering bone morphogenetic proteins.

Jung IH, Lim HC, Lee EU, Lee JS, Jung UW, Choi SH - J Periodontal Implant Sci (2015)

Bottom Line: The new bone formation was significantly greater in the BCP and CBCP groups than in the control and CS groups at 2 weeks, but did not significantly differ among the four groups at 8 week.The CBCP group exhibited more new bone formation in the intergranular space and in the center of the defect compared to the BCP group at 2 weeks, but a similar histologic appearance was observed in both groups at 8 weeks.The dose of rhBMP-2 in the present study enhanced bone regeneration in the early healing period when loaded on BCP and CBCP in rabbit calvarial defects.

View Article: PubMed Central - PubMed

Affiliation: Department of Dental Hygiene, Eulji University College of Health Science, Seongnam, Korea. ; Department of Periodontology, Research Institute for Periodontal Regeneration, Yonsei University College of Dentistry, Seoul, Korea.

ABSTRACT

Purpose: The objective of this study was to comparatively assess the bone regenerative capacity of absorbable collagen sponge (ACS), biphasic calcium phosphate block (BCP) and collagenated biphasic calcium phosphate (CBCP) loaded with a low dose of recombinant human bone morphogenetic protein-2 (rhBMP-2).

Methods: The CBCP was characterized by X-ray diffraction and scanning electron microscopy. In rabbit calvaria, four circular 8-mm-diameter defects were created and assigned to one of four groups: (1) blood-filled group (control), (2) rhBMP-2-soaked absorbable collagen sponge (0.05 mg/mL, 0.1 mL; CS group), (3) rhBMP-2-loaded BCP (BCP group), or (4) rhBMP-2-loaded CBCP (CBCP group). The animals were sacrificed either 2 weeks or 8 weeks postoperatively. Histological and histomorphometric analyses were performed.

Results: The CBCP showed web-like collagen fibrils on and between particles. Greater dimensional stability was observed in the BCP and CBCP groups than in the control and the CS groups at 2 and 8 weeks. The new bone formation was significantly greater in the BCP and CBCP groups than in the control and CS groups at 2 weeks, but did not significantly differ among the four groups at 8 week. The CBCP group exhibited more new bone formation in the intergranular space and in the center of the defect compared to the BCP group at 2 weeks, but a similar histologic appearance was observed in both groups at 8 weeks.

Conclusions: The dose of rhBMP-2 in the present study enhanced bone regeneration in the early healing period when loaded on BCP and CBCP in rabbit calvarial defects.

No MeSH data available.


Histologic observations at 8 weeks postsurgery. (A, B) the control group, (C, D) the collagen sponge (CS) group, (E, F) the biphasic calcium phosphate (BCP) group, (G, H) the collagenated BCP (CBCP) group. (B, D, G, F) Higher magnification views of the boxed area in A, C, E, and G, respectively. The black arrows indicate the margins of the defect. The black and yellow arrowheads indicate osteoblasts and osteoclasts, respectively. Hematoxylin-eosin stain; scale bar (A, C, E, G)= 1 mm, (B, D, G, F)= 200 µm. NB, newly formed bone; RM, residual material.
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Figure 5: Histologic observations at 8 weeks postsurgery. (A, B) the control group, (C, D) the collagen sponge (CS) group, (E, F) the biphasic calcium phosphate (BCP) group, (G, H) the collagenated BCP (CBCP) group. (B, D, G, F) Higher magnification views of the boxed area in A, C, E, and G, respectively. The black arrows indicate the margins of the defect. The black and yellow arrowheads indicate osteoblasts and osteoclasts, respectively. Hematoxylin-eosin stain; scale bar (A, C, E, G)= 1 mm, (B, D, G, F)= 200 µm. NB, newly formed bone; RM, residual material.

Mentions: At 8 weeks, the control group and CS group still exhibited a depressed morphology. Bone ingrowth from the periphery of the defects had increased, and several bony islands could be observed. There was a larger number of bony islands in the CS group than in the control group. No remnants of collagen could be seen in the CS group. In the BCP and CBCP groups, the augmented space was well-maintained, and much of the space formerly filled with residual material and fibrovascular tissues at 2 weeks was replaced by new bone at 8 weeks. New bone formation was observed around the remnant material under the skin flaps in the BCP and CBCP groups. Most of the surface of residual particles was in contact with new bone. Several osteoblasts were observed near the remnant material and the front of new bone. Many osteocytes and reversal lines were observed, along with some empty lacunae (Fig. 5).


Comparative analysis of carrier systems for delivering bone morphogenetic proteins.

Jung IH, Lim HC, Lee EU, Lee JS, Jung UW, Choi SH - J Periodontal Implant Sci (2015)

Histologic observations at 8 weeks postsurgery. (A, B) the control group, (C, D) the collagen sponge (CS) group, (E, F) the biphasic calcium phosphate (BCP) group, (G, H) the collagenated BCP (CBCP) group. (B, D, G, F) Higher magnification views of the boxed area in A, C, E, and G, respectively. The black arrows indicate the margins of the defect. The black and yellow arrowheads indicate osteoblasts and osteoclasts, respectively. Hematoxylin-eosin stain; scale bar (A, C, E, G)= 1 mm, (B, D, G, F)= 200 µm. NB, newly formed bone; RM, residual material.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4556799&req=5

Figure 5: Histologic observations at 8 weeks postsurgery. (A, B) the control group, (C, D) the collagen sponge (CS) group, (E, F) the biphasic calcium phosphate (BCP) group, (G, H) the collagenated BCP (CBCP) group. (B, D, G, F) Higher magnification views of the boxed area in A, C, E, and G, respectively. The black arrows indicate the margins of the defect. The black and yellow arrowheads indicate osteoblasts and osteoclasts, respectively. Hematoxylin-eosin stain; scale bar (A, C, E, G)= 1 mm, (B, D, G, F)= 200 µm. NB, newly formed bone; RM, residual material.
Mentions: At 8 weeks, the control group and CS group still exhibited a depressed morphology. Bone ingrowth from the periphery of the defects had increased, and several bony islands could be observed. There was a larger number of bony islands in the CS group than in the control group. No remnants of collagen could be seen in the CS group. In the BCP and CBCP groups, the augmented space was well-maintained, and much of the space formerly filled with residual material and fibrovascular tissues at 2 weeks was replaced by new bone at 8 weeks. New bone formation was observed around the remnant material under the skin flaps in the BCP and CBCP groups. Most of the surface of residual particles was in contact with new bone. Several osteoblasts were observed near the remnant material and the front of new bone. Many osteocytes and reversal lines were observed, along with some empty lacunae (Fig. 5).

Bottom Line: The new bone formation was significantly greater in the BCP and CBCP groups than in the control and CS groups at 2 weeks, but did not significantly differ among the four groups at 8 week.The CBCP group exhibited more new bone formation in the intergranular space and in the center of the defect compared to the BCP group at 2 weeks, but a similar histologic appearance was observed in both groups at 8 weeks.The dose of rhBMP-2 in the present study enhanced bone regeneration in the early healing period when loaded on BCP and CBCP in rabbit calvarial defects.

View Article: PubMed Central - PubMed

Affiliation: Department of Dental Hygiene, Eulji University College of Health Science, Seongnam, Korea. ; Department of Periodontology, Research Institute for Periodontal Regeneration, Yonsei University College of Dentistry, Seoul, Korea.

ABSTRACT

Purpose: The objective of this study was to comparatively assess the bone regenerative capacity of absorbable collagen sponge (ACS), biphasic calcium phosphate block (BCP) and collagenated biphasic calcium phosphate (CBCP) loaded with a low dose of recombinant human bone morphogenetic protein-2 (rhBMP-2).

Methods: The CBCP was characterized by X-ray diffraction and scanning electron microscopy. In rabbit calvaria, four circular 8-mm-diameter defects were created and assigned to one of four groups: (1) blood-filled group (control), (2) rhBMP-2-soaked absorbable collagen sponge (0.05 mg/mL, 0.1 mL; CS group), (3) rhBMP-2-loaded BCP (BCP group), or (4) rhBMP-2-loaded CBCP (CBCP group). The animals were sacrificed either 2 weeks or 8 weeks postoperatively. Histological and histomorphometric analyses were performed.

Results: The CBCP showed web-like collagen fibrils on and between particles. Greater dimensional stability was observed in the BCP and CBCP groups than in the control and the CS groups at 2 and 8 weeks. The new bone formation was significantly greater in the BCP and CBCP groups than in the control and CS groups at 2 weeks, but did not significantly differ among the four groups at 8 week. The CBCP group exhibited more new bone formation in the intergranular space and in the center of the defect compared to the BCP group at 2 weeks, but a similar histologic appearance was observed in both groups at 8 weeks.

Conclusions: The dose of rhBMP-2 in the present study enhanced bone regeneration in the early healing period when loaded on BCP and CBCP in rabbit calvarial defects.

No MeSH data available.