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Comparative analysis of carrier systems for delivering bone morphogenetic proteins.

Jung IH, Lim HC, Lee EU, Lee JS, Jung UW, Choi SH - J Periodontal Implant Sci (2015)

Bottom Line: The new bone formation was significantly greater in the BCP and CBCP groups than in the control and CS groups at 2 weeks, but did not significantly differ among the four groups at 8 week.The CBCP group exhibited more new bone formation in the intergranular space and in the center of the defect compared to the BCP group at 2 weeks, but a similar histologic appearance was observed in both groups at 8 weeks.The dose of rhBMP-2 in the present study enhanced bone regeneration in the early healing period when loaded on BCP and CBCP in rabbit calvarial defects.

View Article: PubMed Central - PubMed

Affiliation: Department of Dental Hygiene, Eulji University College of Health Science, Seongnam, Korea. ; Department of Periodontology, Research Institute for Periodontal Regeneration, Yonsei University College of Dentistry, Seoul, Korea.

ABSTRACT

Purpose: The objective of this study was to comparatively assess the bone regenerative capacity of absorbable collagen sponge (ACS), biphasic calcium phosphate block (BCP) and collagenated biphasic calcium phosphate (CBCP) loaded with a low dose of recombinant human bone morphogenetic protein-2 (rhBMP-2).

Methods: The CBCP was characterized by X-ray diffraction and scanning electron microscopy. In rabbit calvaria, four circular 8-mm-diameter defects were created and assigned to one of four groups: (1) blood-filled group (control), (2) rhBMP-2-soaked absorbable collagen sponge (0.05 mg/mL, 0.1 mL; CS group), (3) rhBMP-2-loaded BCP (BCP group), or (4) rhBMP-2-loaded CBCP (CBCP group). The animals were sacrificed either 2 weeks or 8 weeks postoperatively. Histological and histomorphometric analyses were performed.

Results: The CBCP showed web-like collagen fibrils on and between particles. Greater dimensional stability was observed in the BCP and CBCP groups than in the control and the CS groups at 2 and 8 weeks. The new bone formation was significantly greater in the BCP and CBCP groups than in the control and CS groups at 2 weeks, but did not significantly differ among the four groups at 8 week. The CBCP group exhibited more new bone formation in the intergranular space and in the center of the defect compared to the BCP group at 2 weeks, but a similar histologic appearance was observed in both groups at 8 weeks.

Conclusions: The dose of rhBMP-2 in the present study enhanced bone regeneration in the early healing period when loaded on BCP and CBCP in rabbit calvarial defects.

No MeSH data available.


Related in: MedlinePlus

Gross view and microstructure of biphasic calcium phosphate (BCP) and collagenated BCP (CBCP). (A) Clinical photographs in wet or dry conditions of BCP and CBCP. (B) Scanning electron microscopy views of BCP and CBCP.
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Figure 3: Gross view and microstructure of biphasic calcium phosphate (BCP) and collagenated BCP (CBCP). (A) Clinical photographs in wet or dry conditions of BCP and CBCP. (B) Scanning electron microscopy views of BCP and CBCP.

Mentions: The phase analysis of the CBCP block is shown in Fig. 2; the 2θ values of the highest two intensity peaks were 31.8° and 31.0°. When they were compared with Joint Committee on Powder Diffraction Standards card Nos. 09-0432 (HA) and 9-0169 (β-TCP), the XRD spectrum of the collagenated BCP corresponded with the composite phases of HA and β-TCP. As shown in gross view and microscopy, CBCP was able to hold easily in both dry and wet conditions and revealed a web-like structure of collagen fibrils on the BCP particles (Fig. 3).


Comparative analysis of carrier systems for delivering bone morphogenetic proteins.

Jung IH, Lim HC, Lee EU, Lee JS, Jung UW, Choi SH - J Periodontal Implant Sci (2015)

Gross view and microstructure of biphasic calcium phosphate (BCP) and collagenated BCP (CBCP). (A) Clinical photographs in wet or dry conditions of BCP and CBCP. (B) Scanning electron microscopy views of BCP and CBCP.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4556799&req=5

Figure 3: Gross view and microstructure of biphasic calcium phosphate (BCP) and collagenated BCP (CBCP). (A) Clinical photographs in wet or dry conditions of BCP and CBCP. (B) Scanning electron microscopy views of BCP and CBCP.
Mentions: The phase analysis of the CBCP block is shown in Fig. 2; the 2θ values of the highest two intensity peaks were 31.8° and 31.0°. When they were compared with Joint Committee on Powder Diffraction Standards card Nos. 09-0432 (HA) and 9-0169 (β-TCP), the XRD spectrum of the collagenated BCP corresponded with the composite phases of HA and β-TCP. As shown in gross view and microscopy, CBCP was able to hold easily in both dry and wet conditions and revealed a web-like structure of collagen fibrils on the BCP particles (Fig. 3).

Bottom Line: The new bone formation was significantly greater in the BCP and CBCP groups than in the control and CS groups at 2 weeks, but did not significantly differ among the four groups at 8 week.The CBCP group exhibited more new bone formation in the intergranular space and in the center of the defect compared to the BCP group at 2 weeks, but a similar histologic appearance was observed in both groups at 8 weeks.The dose of rhBMP-2 in the present study enhanced bone regeneration in the early healing period when loaded on BCP and CBCP in rabbit calvarial defects.

View Article: PubMed Central - PubMed

Affiliation: Department of Dental Hygiene, Eulji University College of Health Science, Seongnam, Korea. ; Department of Periodontology, Research Institute for Periodontal Regeneration, Yonsei University College of Dentistry, Seoul, Korea.

ABSTRACT

Purpose: The objective of this study was to comparatively assess the bone regenerative capacity of absorbable collagen sponge (ACS), biphasic calcium phosphate block (BCP) and collagenated biphasic calcium phosphate (CBCP) loaded with a low dose of recombinant human bone morphogenetic protein-2 (rhBMP-2).

Methods: The CBCP was characterized by X-ray diffraction and scanning electron microscopy. In rabbit calvaria, four circular 8-mm-diameter defects were created and assigned to one of four groups: (1) blood-filled group (control), (2) rhBMP-2-soaked absorbable collagen sponge (0.05 mg/mL, 0.1 mL; CS group), (3) rhBMP-2-loaded BCP (BCP group), or (4) rhBMP-2-loaded CBCP (CBCP group). The animals were sacrificed either 2 weeks or 8 weeks postoperatively. Histological and histomorphometric analyses were performed.

Results: The CBCP showed web-like collagen fibrils on and between particles. Greater dimensional stability was observed in the BCP and CBCP groups than in the control and the CS groups at 2 and 8 weeks. The new bone formation was significantly greater in the BCP and CBCP groups than in the control and CS groups at 2 weeks, but did not significantly differ among the four groups at 8 week. The CBCP group exhibited more new bone formation in the intergranular space and in the center of the defect compared to the BCP group at 2 weeks, but a similar histologic appearance was observed in both groups at 8 weeks.

Conclusions: The dose of rhBMP-2 in the present study enhanced bone regeneration in the early healing period when loaded on BCP and CBCP in rabbit calvarial defects.

No MeSH data available.


Related in: MedlinePlus