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Inhibition of DNA nanotube-conjugated mTOR siRNA on the growth of pulmonary arterial smooth muscle cells.

You Z, Qian H, Wang C, He B, Yan J, Mao C, Wang G - Data Brief (2015)

Bottom Line: Here we provide raw and processed data and methods behind mTOR siRNA loaded DNA nanotubes (siRNA-DNA-NTs) in the growth of pulmonary arterial smooth muscle cells (PASMCs) under both normoxic and hypoxic condition, and also related to (You et al., Biomaterials, 2015, 67:137-150, [1]).The MTT analysis, Semi-quantitative RT-PCR data presented here were used to probe cytotoxicity of mTOR siRNA-DNA-NT complex in its TAE-Mg(2+) buffer. siRNA-DNA-NTs have a lower cytotoxicity and higher transfection efficiency and can, based on inhibition of mTOR expression, decrease PASMCs growth both hypoxic and normal condition.

View Article: PubMed Central - PubMed

Affiliation: Institute of Respiratory Diseases, Xinqiao Hospital, Third Military Medical University, Chongqing 400037, China ; Department of Emergency, Xinqiao Hospital, Chongqing 400037, China.

ABSTRACT
Here we provide raw and processed data and methods behind mTOR siRNA loaded DNA nanotubes (siRNA-DNA-NTs) in the growth of pulmonary arterial smooth muscle cells (PASMCs) under both normoxic and hypoxic condition, and also related to (You et al., Biomaterials, 2015, 67:137-150, [1]). The MTT analysis, Semi-quantitative RT-PCR data presented here were used to probe cytotoxicity of mTOR siRNA-DNA-NT complex in its TAE-Mg(2+) buffer. siRNA-DNA-NTs have a lower cytotoxicity and higher transfection efficiency and can, based on inhibition of mTOR expression, decrease PASMCs growth both hypoxic and normal condition.

No MeSH data available.


Related in: MedlinePlus

Inhibition of mTOR siRNA-DNA-NTs on the mTOR expression in PASMCs. The data represent the mean±S.E. (n=3). (A) *p<0.05 versus control group and ^p<0.05 versus the siRNA alone group. (B) *p<0.05 versus the 0 h group and ^p<0.05 versus the group incubated for 24 h. (C) *p<0.05 versus the 0 nM group and ^p<0.05 versus the 25 nM group.
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f0025: Inhibition of mTOR siRNA-DNA-NTs on the mTOR expression in PASMCs. The data represent the mean±S.E. (n=3). (A) *p<0.05 versus control group and ^p<0.05 versus the siRNA alone group. (B) *p<0.05 versus the 0 h group and ^p<0.05 versus the group incubated for 24 h. (C) *p<0.05 versus the 0 nM group and ^p<0.05 versus the 25 nM group.

Mentions: Fig. 5 reveals the inhibition of mTOR siRNA-loaded nanotubes on mTOR expression in PASMCs. First, comparison of the inhibition of mTOR siRNA with or without DNA-NTs in PASMCs is in Fig. 5A. PASMCs were incubated with 50 nM siRNA alone and siRNA-DNA-NTs for 48 h. Representative bands show the expression of mTOR mRNA (upper panel) and β-actin (middle panel). The histograms in the lower panel illustrate the levels of mTOR, which were normalized to the β-actin expression. Second, mTOR mRNA levels were decreased in a time-dependent manner (Fig. 5B). PASMCs were incubated with 50 nM siRNA-loaded DNA-NTs for a predetermined amount of time (0, 24, 48, 72 h). Representative bands show the expression of mTOR mRNA (upper panel) and β-actin (middle panel). The histograms in the lower panel illustrate the levels of mTOR, which were normalized to the β-actin expression. Third, mTOR mRNA levels were suppressed in a concentration-dependent manner (Fig. 5C). PASMCs were incubated with various concentrations of siRNA-DNA-NTs (0, 12.5, 25, 100 µM) for 48 h. Representative bands show the expression of mTOR mRNA (upper panel) and β-actin (middle panel). The histograms in the lower panel illustrate the levels of mTOR, which were normalized to the β-actin expression.


Inhibition of DNA nanotube-conjugated mTOR siRNA on the growth of pulmonary arterial smooth muscle cells.

You Z, Qian H, Wang C, He B, Yan J, Mao C, Wang G - Data Brief (2015)

Inhibition of mTOR siRNA-DNA-NTs on the mTOR expression in PASMCs. The data represent the mean±S.E. (n=3). (A) *p<0.05 versus control group and ^p<0.05 versus the siRNA alone group. (B) *p<0.05 versus the 0 h group and ^p<0.05 versus the group incubated for 24 h. (C) *p<0.05 versus the 0 nM group and ^p<0.05 versus the 25 nM group.
© Copyright Policy - CC BY
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4556778&req=5

f0025: Inhibition of mTOR siRNA-DNA-NTs on the mTOR expression in PASMCs. The data represent the mean±S.E. (n=3). (A) *p<0.05 versus control group and ^p<0.05 versus the siRNA alone group. (B) *p<0.05 versus the 0 h group and ^p<0.05 versus the group incubated for 24 h. (C) *p<0.05 versus the 0 nM group and ^p<0.05 versus the 25 nM group.
Mentions: Fig. 5 reveals the inhibition of mTOR siRNA-loaded nanotubes on mTOR expression in PASMCs. First, comparison of the inhibition of mTOR siRNA with or without DNA-NTs in PASMCs is in Fig. 5A. PASMCs were incubated with 50 nM siRNA alone and siRNA-DNA-NTs for 48 h. Representative bands show the expression of mTOR mRNA (upper panel) and β-actin (middle panel). The histograms in the lower panel illustrate the levels of mTOR, which were normalized to the β-actin expression. Second, mTOR mRNA levels were decreased in a time-dependent manner (Fig. 5B). PASMCs were incubated with 50 nM siRNA-loaded DNA-NTs for a predetermined amount of time (0, 24, 48, 72 h). Representative bands show the expression of mTOR mRNA (upper panel) and β-actin (middle panel). The histograms in the lower panel illustrate the levels of mTOR, which were normalized to the β-actin expression. Third, mTOR mRNA levels were suppressed in a concentration-dependent manner (Fig. 5C). PASMCs were incubated with various concentrations of siRNA-DNA-NTs (0, 12.5, 25, 100 µM) for 48 h. Representative bands show the expression of mTOR mRNA (upper panel) and β-actin (middle panel). The histograms in the lower panel illustrate the levels of mTOR, which were normalized to the β-actin expression.

Bottom Line: Here we provide raw and processed data and methods behind mTOR siRNA loaded DNA nanotubes (siRNA-DNA-NTs) in the growth of pulmonary arterial smooth muscle cells (PASMCs) under both normoxic and hypoxic condition, and also related to (You et al., Biomaterials, 2015, 67:137-150, [1]).The MTT analysis, Semi-quantitative RT-PCR data presented here were used to probe cytotoxicity of mTOR siRNA-DNA-NT complex in its TAE-Mg(2+) buffer. siRNA-DNA-NTs have a lower cytotoxicity and higher transfection efficiency and can, based on inhibition of mTOR expression, decrease PASMCs growth both hypoxic and normal condition.

View Article: PubMed Central - PubMed

Affiliation: Institute of Respiratory Diseases, Xinqiao Hospital, Third Military Medical University, Chongqing 400037, China ; Department of Emergency, Xinqiao Hospital, Chongqing 400037, China.

ABSTRACT
Here we provide raw and processed data and methods behind mTOR siRNA loaded DNA nanotubes (siRNA-DNA-NTs) in the growth of pulmonary arterial smooth muscle cells (PASMCs) under both normoxic and hypoxic condition, and also related to (You et al., Biomaterials, 2015, 67:137-150, [1]). The MTT analysis, Semi-quantitative RT-PCR data presented here were used to probe cytotoxicity of mTOR siRNA-DNA-NT complex in its TAE-Mg(2+) buffer. siRNA-DNA-NTs have a lower cytotoxicity and higher transfection efficiency and can, based on inhibition of mTOR expression, decrease PASMCs growth both hypoxic and normal condition.

No MeSH data available.


Related in: MedlinePlus