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Inhibition of DNA nanotube-conjugated mTOR siRNA on the growth of pulmonary arterial smooth muscle cells.

You Z, Qian H, Wang C, He B, Yan J, Mao C, Wang G - Data Brief (2015)

Bottom Line: Here we provide raw and processed data and methods behind mTOR siRNA loaded DNA nanotubes (siRNA-DNA-NTs) in the growth of pulmonary arterial smooth muscle cells (PASMCs) under both normoxic and hypoxic condition, and also related to (You et al., Biomaterials, 2015, 67:137-150, [1]).The MTT analysis, Semi-quantitative RT-PCR data presented here were used to probe cytotoxicity of mTOR siRNA-DNA-NT complex in its TAE-Mg(2+) buffer. siRNA-DNA-NTs have a lower cytotoxicity and higher transfection efficiency and can, based on inhibition of mTOR expression, decrease PASMCs growth both hypoxic and normal condition.

View Article: PubMed Central - PubMed

Affiliation: Institute of Respiratory Diseases, Xinqiao Hospital, Third Military Medical University, Chongqing 400037, China ; Department of Emergency, Xinqiao Hospital, Chongqing 400037, China.

ABSTRACT
Here we provide raw and processed data and methods behind mTOR siRNA loaded DNA nanotubes (siRNA-DNA-NTs) in the growth of pulmonary arterial smooth muscle cells (PASMCs) under both normoxic and hypoxic condition, and also related to (You et al., Biomaterials, 2015, 67:137-150, [1]). The MTT analysis, Semi-quantitative RT-PCR data presented here were used to probe cytotoxicity of mTOR siRNA-DNA-NT complex in its TAE-Mg(2+) buffer. siRNA-DNA-NTs have a lower cytotoxicity and higher transfection efficiency and can, based on inhibition of mTOR expression, decrease PASMCs growth both hypoxic and normal condition.

No MeSH data available.


Related in: MedlinePlus

Effect of mTOR siRNA-DNA-NTs on the growth of PASMCs under hypoxic condition. The cell viability was assessed by MTT assay. All of the data were normalized to the mean count numbers under normoxia. The data are the mean±S.E. (n=4). *p<0.05 versus the normal group (0 h), ^p<0.05 versus the group with 24 h of treatment, and #p<0.05 versus the corresponding hypoxic group at 24 or 48 h.
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f0020: Effect of mTOR siRNA-DNA-NTs on the growth of PASMCs under hypoxic condition. The cell viability was assessed by MTT assay. All of the data were normalized to the mean count numbers under normoxia. The data are the mean±S.E. (n=4). *p<0.05 versus the normal group (0 h), ^p<0.05 versus the group with 24 h of treatment, and #p<0.05 versus the corresponding hypoxic group at 24 or 48 h.

Mentions: Fig. 4 shows the viability of PASMCs incubated with 50 nM of mTOR siRNA treatment for predetermined amounts of time (0, 12, 24, 48 h) by the MTT assay. Cells were first cultured in 1% serum for 16 h and were then transfected with 50 nM of mTOR siRNA treatment, followed by exposure to hypoxia for another 0, 12, 24, or 48 h. Specifically, the PASMCs were pre-cultured with 3-MA (5 mM) for 30 min and then transfected with the siRNA-DNA-NTs. MTT assay showed that the rate of cell growth decreased by 11%, 42%, and 78% compared with correspondent alone hypoxic group at 12, 24, 48 h, respectively.


Inhibition of DNA nanotube-conjugated mTOR siRNA on the growth of pulmonary arterial smooth muscle cells.

You Z, Qian H, Wang C, He B, Yan J, Mao C, Wang G - Data Brief (2015)

Effect of mTOR siRNA-DNA-NTs on the growth of PASMCs under hypoxic condition. The cell viability was assessed by MTT assay. All of the data were normalized to the mean count numbers under normoxia. The data are the mean±S.E. (n=4). *p<0.05 versus the normal group (0 h), ^p<0.05 versus the group with 24 h of treatment, and #p<0.05 versus the corresponding hypoxic group at 24 or 48 h.
© Copyright Policy - CC BY
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4556778&req=5

f0020: Effect of mTOR siRNA-DNA-NTs on the growth of PASMCs under hypoxic condition. The cell viability was assessed by MTT assay. All of the data were normalized to the mean count numbers under normoxia. The data are the mean±S.E. (n=4). *p<0.05 versus the normal group (0 h), ^p<0.05 versus the group with 24 h of treatment, and #p<0.05 versus the corresponding hypoxic group at 24 or 48 h.
Mentions: Fig. 4 shows the viability of PASMCs incubated with 50 nM of mTOR siRNA treatment for predetermined amounts of time (0, 12, 24, 48 h) by the MTT assay. Cells were first cultured in 1% serum for 16 h and were then transfected with 50 nM of mTOR siRNA treatment, followed by exposure to hypoxia for another 0, 12, 24, or 48 h. Specifically, the PASMCs were pre-cultured with 3-MA (5 mM) for 30 min and then transfected with the siRNA-DNA-NTs. MTT assay showed that the rate of cell growth decreased by 11%, 42%, and 78% compared with correspondent alone hypoxic group at 12, 24, 48 h, respectively.

Bottom Line: Here we provide raw and processed data and methods behind mTOR siRNA loaded DNA nanotubes (siRNA-DNA-NTs) in the growth of pulmonary arterial smooth muscle cells (PASMCs) under both normoxic and hypoxic condition, and also related to (You et al., Biomaterials, 2015, 67:137-150, [1]).The MTT analysis, Semi-quantitative RT-PCR data presented here were used to probe cytotoxicity of mTOR siRNA-DNA-NT complex in its TAE-Mg(2+) buffer. siRNA-DNA-NTs have a lower cytotoxicity and higher transfection efficiency and can, based on inhibition of mTOR expression, decrease PASMCs growth both hypoxic and normal condition.

View Article: PubMed Central - PubMed

Affiliation: Institute of Respiratory Diseases, Xinqiao Hospital, Third Military Medical University, Chongqing 400037, China ; Department of Emergency, Xinqiao Hospital, Chongqing 400037, China.

ABSTRACT
Here we provide raw and processed data and methods behind mTOR siRNA loaded DNA nanotubes (siRNA-DNA-NTs) in the growth of pulmonary arterial smooth muscle cells (PASMCs) under both normoxic and hypoxic condition, and also related to (You et al., Biomaterials, 2015, 67:137-150, [1]). The MTT analysis, Semi-quantitative RT-PCR data presented here were used to probe cytotoxicity of mTOR siRNA-DNA-NT complex in its TAE-Mg(2+) buffer. siRNA-DNA-NTs have a lower cytotoxicity and higher transfection efficiency and can, based on inhibition of mTOR expression, decrease PASMCs growth both hypoxic and normal condition.

No MeSH data available.


Related in: MedlinePlus