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Inhibition of DNA nanotube-conjugated mTOR siRNA on the growth of pulmonary arterial smooth muscle cells.

You Z, Qian H, Wang C, He B, Yan J, Mao C, Wang G - Data Brief (2015)

Bottom Line: Here we provide raw and processed data and methods behind mTOR siRNA loaded DNA nanotubes (siRNA-DNA-NTs) in the growth of pulmonary arterial smooth muscle cells (PASMCs) under both normoxic and hypoxic condition, and also related to (You et al., Biomaterials, 2015, 67:137-150, [1]).The MTT analysis, Semi-quantitative RT-PCR data presented here were used to probe cytotoxicity of mTOR siRNA-DNA-NT complex in its TAE-Mg(2+) buffer. siRNA-DNA-NTs have a lower cytotoxicity and higher transfection efficiency and can, based on inhibition of mTOR expression, decrease PASMCs growth both hypoxic and normal condition.

View Article: PubMed Central - PubMed

Affiliation: Institute of Respiratory Diseases, Xinqiao Hospital, Third Military Medical University, Chongqing 400037, China ; Department of Emergency, Xinqiao Hospital, Chongqing 400037, China.

ABSTRACT
Here we provide raw and processed data and methods behind mTOR siRNA loaded DNA nanotubes (siRNA-DNA-NTs) in the growth of pulmonary arterial smooth muscle cells (PASMCs) under both normoxic and hypoxic condition, and also related to (You et al., Biomaterials, 2015, 67:137-150, [1]). The MTT analysis, Semi-quantitative RT-PCR data presented here were used to probe cytotoxicity of mTOR siRNA-DNA-NT complex in its TAE-Mg(2+) buffer. siRNA-DNA-NTs have a lower cytotoxicity and higher transfection efficiency and can, based on inhibition of mTOR expression, decrease PASMCs growth both hypoxic and normal condition.

No MeSH data available.


Related in: MedlinePlus

Cell growth inhibition by mTOR siRNA-loaded nanotubes under normoxic condition. The data represent the mean±S.E. (n=4). *p<0.05 versus the 0 nM group and ^p<0.05 versus the 25 nM group.
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f0015: Cell growth inhibition by mTOR siRNA-loaded nanotubes under normoxic condition. The data represent the mean±S.E. (n=4). *p<0.05 versus the 0 nM group and ^p<0.05 versus the 25 nM group.

Mentions: Fig. 3 shows in normal condition, that cell growth inhibition of mTOR siRNA-loaded nanotubes is in a time- and dose-dependent manner using MTT method. The histogram display that the cells were treated by mTOR siRNA-loaded nanotubes at doses of 12.5, 25, 50, and 100 nM, respectively, for 0, 12, 24, and 48 h, respectively. By treatment with 50 nM of mTOR siRNA loaded DNA-NTs, the inhibition rate of cell growth was 2.4%, 11.9%, 27.3%, 34.6%, 46.8% at 12, 24, 36, 48 h, respectively.


Inhibition of DNA nanotube-conjugated mTOR siRNA on the growth of pulmonary arterial smooth muscle cells.

You Z, Qian H, Wang C, He B, Yan J, Mao C, Wang G - Data Brief (2015)

Cell growth inhibition by mTOR siRNA-loaded nanotubes under normoxic condition. The data represent the mean±S.E. (n=4). *p<0.05 versus the 0 nM group and ^p<0.05 versus the 25 nM group.
© Copyright Policy - CC BY
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4556778&req=5

f0015: Cell growth inhibition by mTOR siRNA-loaded nanotubes under normoxic condition. The data represent the mean±S.E. (n=4). *p<0.05 versus the 0 nM group and ^p<0.05 versus the 25 nM group.
Mentions: Fig. 3 shows in normal condition, that cell growth inhibition of mTOR siRNA-loaded nanotubes is in a time- and dose-dependent manner using MTT method. The histogram display that the cells were treated by mTOR siRNA-loaded nanotubes at doses of 12.5, 25, 50, and 100 nM, respectively, for 0, 12, 24, and 48 h, respectively. By treatment with 50 nM of mTOR siRNA loaded DNA-NTs, the inhibition rate of cell growth was 2.4%, 11.9%, 27.3%, 34.6%, 46.8% at 12, 24, 36, 48 h, respectively.

Bottom Line: Here we provide raw and processed data and methods behind mTOR siRNA loaded DNA nanotubes (siRNA-DNA-NTs) in the growth of pulmonary arterial smooth muscle cells (PASMCs) under both normoxic and hypoxic condition, and also related to (You et al., Biomaterials, 2015, 67:137-150, [1]).The MTT analysis, Semi-quantitative RT-PCR data presented here were used to probe cytotoxicity of mTOR siRNA-DNA-NT complex in its TAE-Mg(2+) buffer. siRNA-DNA-NTs have a lower cytotoxicity and higher transfection efficiency and can, based on inhibition of mTOR expression, decrease PASMCs growth both hypoxic and normal condition.

View Article: PubMed Central - PubMed

Affiliation: Institute of Respiratory Diseases, Xinqiao Hospital, Third Military Medical University, Chongqing 400037, China ; Department of Emergency, Xinqiao Hospital, Chongqing 400037, China.

ABSTRACT
Here we provide raw and processed data and methods behind mTOR siRNA loaded DNA nanotubes (siRNA-DNA-NTs) in the growth of pulmonary arterial smooth muscle cells (PASMCs) under both normoxic and hypoxic condition, and also related to (You et al., Biomaterials, 2015, 67:137-150, [1]). The MTT analysis, Semi-quantitative RT-PCR data presented here were used to probe cytotoxicity of mTOR siRNA-DNA-NT complex in its TAE-Mg(2+) buffer. siRNA-DNA-NTs have a lower cytotoxicity and higher transfection efficiency and can, based on inhibition of mTOR expression, decrease PASMCs growth both hypoxic and normal condition.

No MeSH data available.


Related in: MedlinePlus