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Inhibition of DNA nanotube-conjugated mTOR siRNA on the growth of pulmonary arterial smooth muscle cells.

You Z, Qian H, Wang C, He B, Yan J, Mao C, Wang G - Data Brief (2015)

Bottom Line: Here we provide raw and processed data and methods behind mTOR siRNA loaded DNA nanotubes (siRNA-DNA-NTs) in the growth of pulmonary arterial smooth muscle cells (PASMCs) under both normoxic and hypoxic condition, and also related to (You et al., Biomaterials, 2015, 67:137-150, [1]).The MTT analysis, Semi-quantitative RT-PCR data presented here were used to probe cytotoxicity of mTOR siRNA-DNA-NT complex in its TAE-Mg(2+) buffer. siRNA-DNA-NTs have a lower cytotoxicity and higher transfection efficiency and can, based on inhibition of mTOR expression, decrease PASMCs growth both hypoxic and normal condition.

View Article: PubMed Central - PubMed

Affiliation: Institute of Respiratory Diseases, Xinqiao Hospital, Third Military Medical University, Chongqing 400037, China ; Department of Emergency, Xinqiao Hospital, Chongqing 400037, China.

ABSTRACT
Here we provide raw and processed data and methods behind mTOR siRNA loaded DNA nanotubes (siRNA-DNA-NTs) in the growth of pulmonary arterial smooth muscle cells (PASMCs) under both normoxic and hypoxic condition, and also related to (You et al., Biomaterials, 2015, 67:137-150, [1]). The MTT analysis, Semi-quantitative RT-PCR data presented here were used to probe cytotoxicity of mTOR siRNA-DNA-NT complex in its TAE-Mg(2+) buffer. siRNA-DNA-NTs have a lower cytotoxicity and higher transfection efficiency and can, based on inhibition of mTOR expression, decrease PASMCs growth both hypoxic and normal condition.

No MeSH data available.


Related in: MedlinePlus

The cytotoxicity of DNA nanotube solution and the solvent (1×TAE-Mg2+) at various concentrations in PASMCs. The data represent the mean±S.E. (n=5).
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f0005: The cytotoxicity of DNA nanotube solution and the solvent (1×TAE-Mg2+) at various concentrations in PASMCs. The data represent the mean±S.E. (n=5).

Mentions: In Fig. 1, the cytotoxicity of DNA nanotube solution and the solvent (1×TAE-Mg2+) at various concentrations in PASMCs is represented. The IC50 of DNA nanotubes was found to be 1071.4 nM (Fig. 1A). While the IC50 of the 1× TAE-Mg2+ buffer used for the DNA-NTs was approximately 0.775×buffer (≈930 nM). The cytotoxicity of siRNA-loaded DNA-NT complex, mainly due to its TAE-Mg2+ buffer, was much lower to 141 nM by the pure nanomaterials (Fig. 1B).


Inhibition of DNA nanotube-conjugated mTOR siRNA on the growth of pulmonary arterial smooth muscle cells.

You Z, Qian H, Wang C, He B, Yan J, Mao C, Wang G - Data Brief (2015)

The cytotoxicity of DNA nanotube solution and the solvent (1×TAE-Mg2+) at various concentrations in PASMCs. The data represent the mean±S.E. (n=5).
© Copyright Policy - CC BY
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4556778&req=5

f0005: The cytotoxicity of DNA nanotube solution and the solvent (1×TAE-Mg2+) at various concentrations in PASMCs. The data represent the mean±S.E. (n=5).
Mentions: In Fig. 1, the cytotoxicity of DNA nanotube solution and the solvent (1×TAE-Mg2+) at various concentrations in PASMCs is represented. The IC50 of DNA nanotubes was found to be 1071.4 nM (Fig. 1A). While the IC50 of the 1× TAE-Mg2+ buffer used for the DNA-NTs was approximately 0.775×buffer (≈930 nM). The cytotoxicity of siRNA-loaded DNA-NT complex, mainly due to its TAE-Mg2+ buffer, was much lower to 141 nM by the pure nanomaterials (Fig. 1B).

Bottom Line: Here we provide raw and processed data and methods behind mTOR siRNA loaded DNA nanotubes (siRNA-DNA-NTs) in the growth of pulmonary arterial smooth muscle cells (PASMCs) under both normoxic and hypoxic condition, and also related to (You et al., Biomaterials, 2015, 67:137-150, [1]).The MTT analysis, Semi-quantitative RT-PCR data presented here were used to probe cytotoxicity of mTOR siRNA-DNA-NT complex in its TAE-Mg(2+) buffer. siRNA-DNA-NTs have a lower cytotoxicity and higher transfection efficiency and can, based on inhibition of mTOR expression, decrease PASMCs growth both hypoxic and normal condition.

View Article: PubMed Central - PubMed

Affiliation: Institute of Respiratory Diseases, Xinqiao Hospital, Third Military Medical University, Chongqing 400037, China ; Department of Emergency, Xinqiao Hospital, Chongqing 400037, China.

ABSTRACT
Here we provide raw and processed data and methods behind mTOR siRNA loaded DNA nanotubes (siRNA-DNA-NTs) in the growth of pulmonary arterial smooth muscle cells (PASMCs) under both normoxic and hypoxic condition, and also related to (You et al., Biomaterials, 2015, 67:137-150, [1]). The MTT analysis, Semi-quantitative RT-PCR data presented here were used to probe cytotoxicity of mTOR siRNA-DNA-NT complex in its TAE-Mg(2+) buffer. siRNA-DNA-NTs have a lower cytotoxicity and higher transfection efficiency and can, based on inhibition of mTOR expression, decrease PASMCs growth both hypoxic and normal condition.

No MeSH data available.


Related in: MedlinePlus