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MiR-125b inhibits stromal cell proliferation in giant cell tumor of bone by targeting parathyroid hormone 1 receptor.

Wu PF, Liang JY, Yu F, Zhou ZB, Tang JY, Li KH - Iran J Basic Med Sci (2015)

Bottom Line: We found that miR-125b was significantly down-regulated in GCT tissues.Our results suggest that miR-125b acts as a tumor suppressor through suppression of the PTH1R/RANKL signaling pathway.These findings contribute to our understanding of the functions of miR-125b in GCT.

View Article: PubMed Central - PubMed

Affiliation: Department of Orthopaedics, Xiang Ya Hospital, Central South University, Changsha 410008, China.

ABSTRACT

Objectives: miR-125b has been identified as a tumor suppressor in many tumors, but its role in giant cell tumor (GCT) of bone remains poorly understood. The current study aimed to investigate the potential role and mechanism of miR-125b in GCT.

Materials and methods: Expression levels of miR-125b in GCT tissues were determined using RT-PCR. The cell proliferation was surveyed by direct cell counting, MTS and CCK-8, and the apoptotic cells were evaluated by Annexin V-FITC and propidium iodine staining assay. The target gene expression was determined using RT-PCR and western blot. Parathyroid hormone 1 receptor (PTH1R) 3'-UTR was cloned into luciferase reporter plasmid to confirm direct targeting.

Results: We found that miR-125b was significantly down-regulated in GCT tissues. Using both gain- and loss-of-function analyses, we further revealed that miR-125b suppressed GCT stromal cell proliferation and induced cell apoptosis. Furthermore, we revealed that PTH/PTHrP type 1 receptor is a direct and functional target of miR-125b.

Conclusion: Our results suggest that miR-125b acts as a tumor suppressor through suppression of the PTH1R/RANKL signaling pathway. These findings contribute to our understanding of the functions of miR-125b in GCT.

No MeSH data available.


Related in: MedlinePlus

The expression of miR-125b in human GCT tissues. miR-125b was detected in 12 GCT patients by qRT-PCR
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Figure 1: The expression of miR-125b in human GCT tissues. miR-125b was detected in 12 GCT patients by qRT-PCR

Mentions: First, the expression pattern of miR-125b in GCT was investigated. Expression levels of miR-125b are examined in 12 pairs of GCT tissues and adjacent normal bone tissues by quantitative RT-PCR analysis (qRT-PCR). As shown in Figure 1, miR-125b was significantly down-regulated in GCT tissues compared to the paired non-tumor tissues (P=0.006, paired t test).


MiR-125b inhibits stromal cell proliferation in giant cell tumor of bone by targeting parathyroid hormone 1 receptor.

Wu PF, Liang JY, Yu F, Zhou ZB, Tang JY, Li KH - Iran J Basic Med Sci (2015)

The expression of miR-125b in human GCT tissues. miR-125b was detected in 12 GCT patients by qRT-PCR
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4556765&req=5

Figure 1: The expression of miR-125b in human GCT tissues. miR-125b was detected in 12 GCT patients by qRT-PCR
Mentions: First, the expression pattern of miR-125b in GCT was investigated. Expression levels of miR-125b are examined in 12 pairs of GCT tissues and adjacent normal bone tissues by quantitative RT-PCR analysis (qRT-PCR). As shown in Figure 1, miR-125b was significantly down-regulated in GCT tissues compared to the paired non-tumor tissues (P=0.006, paired t test).

Bottom Line: We found that miR-125b was significantly down-regulated in GCT tissues.Our results suggest that miR-125b acts as a tumor suppressor through suppression of the PTH1R/RANKL signaling pathway.These findings contribute to our understanding of the functions of miR-125b in GCT.

View Article: PubMed Central - PubMed

Affiliation: Department of Orthopaedics, Xiang Ya Hospital, Central South University, Changsha 410008, China.

ABSTRACT

Objectives: miR-125b has been identified as a tumor suppressor in many tumors, but its role in giant cell tumor (GCT) of bone remains poorly understood. The current study aimed to investigate the potential role and mechanism of miR-125b in GCT.

Materials and methods: Expression levels of miR-125b in GCT tissues were determined using RT-PCR. The cell proliferation was surveyed by direct cell counting, MTS and CCK-8, and the apoptotic cells were evaluated by Annexin V-FITC and propidium iodine staining assay. The target gene expression was determined using RT-PCR and western blot. Parathyroid hormone 1 receptor (PTH1R) 3'-UTR was cloned into luciferase reporter plasmid to confirm direct targeting.

Results: We found that miR-125b was significantly down-regulated in GCT tissues. Using both gain- and loss-of-function analyses, we further revealed that miR-125b suppressed GCT stromal cell proliferation and induced cell apoptosis. Furthermore, we revealed that PTH/PTHrP type 1 receptor is a direct and functional target of miR-125b.

Conclusion: Our results suggest that miR-125b acts as a tumor suppressor through suppression of the PTH1R/RANKL signaling pathway. These findings contribute to our understanding of the functions of miR-125b in GCT.

No MeSH data available.


Related in: MedlinePlus