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Hawthorn ethanolic extracts with triterpenoids and flavonoids exert hepatoprotective effects and suppress the hypercholesterolemia-induced oxidative stress in rats.

Rezaei-Golmisheh A, Malekinejad H, Asri-Rezaei S, Farshid AA, Akbari P - Iran J Basic Med Sci (2015)

Bottom Line: The highest phenol content, oleanolic acid, quercetin and lupeol levels and free radical scavenging potency were found in the bark extract, and the highest ursolic acid level was found in the berries extract.Hawthorn's extracts protected from hepatic thiol depletion and improved the lipid profile and hepatic damages.Moreover, the hypocholesterolemic effect of extracts was found comparable to orlistat.

View Article: PubMed Central - PubMed

Affiliation: Department of Histology and Embryology, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran.

ABSTRACT

Objectives: The current study was aimed to determine the bioactive constituents and biological effects of the Crataegus monogyna ethanolic extracts from bark, leaves and berries on hypercholesterolemia.

Materials and methods: Oleanolic acid, ursolic acid, quercetin and lupeol concentrations were quantified by HPLC. Total phenol content and radical scavenging activity of extracts were also measured. The hypocholesterolemic, antioxidant, and hepatoprotective effects of the extracts were examined in hypercholesterolemic rats and compared with orlistat.

Results: The highest phenol content, oleanolic acid, quercetin and lupeol levels and free radical scavenging potency were found in the bark extract, and the highest ursolic acid level was found in the berries extract. Orlistat and extracts significantly (P<0.05) lowered the hypercholesterolemia-increased serum level of hepatic enzymes and lipid peroxidation level. Hawthorn's extracts protected from hepatic thiol depletion and improved the lipid profile and hepatic damages.

Conclusion: Data suggested that hawthorn's extracts are able to protect from hypercholesterolemia-induced oxidative stress and hepatic injuries. Moreover, the hypocholesterolemic effect of extracts was found comparable to orlistat.

No MeSH data available.


Related in: MedlinePlus

Histopathological findings of rat livers from different treatment groups. (A and B): Control, (C and D): hypercholesterolemic group (HC), (E and F): T1, (G and H): T2, (I and J): T3, (K and L): T4. a: congestion, b: bile ducts hyperplasia, c: inflammatory cells infiltration, d: vacoulation and e: pyknotic nuclei. [H&E staining; (A, C, E, G, I and K ×100) and (B, D, F, H, J and L ×400)]
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Figure 3: Histopathological findings of rat livers from different treatment groups. (A and B): Control, (C and D): hypercholesterolemic group (HC), (E and F): T1, (G and H): T2, (I and J): T3, (K and L): T4. a: congestion, b: bile ducts hyperplasia, c: inflammatory cells infiltration, d: vacoulation and e: pyknotic nuclei. [H&E staining; (A, C, E, G, I and K ×100) and (B, D, F, H, J and L ×400)]

Mentions: The normal histological feature of the liver from the control (C) rats are depicted in Figure 3-A and 3-B. Histopathological findings in the liver of HC animals represented congested blood vessels, bile duct hyperplasia, inflammatory cells infiltration (perivascular cuffing), highly vacuolated hepatocytes and pyknotic nuclei (Figure 3C, and 3D). Administration of bark and leaves extracts although reduced the HCD-induced damages in the liver, there are however symptoms of congestion, perivascular cuffing and vacuolated hepatocytes (Figure 3E, 3F, 3G and 3H, respectively). We found that 4 weeks administration of hawthorn berries extract could fairly improve the HCD-induced hepatic damages, and the liver looks normal (Figure 3-I and 3-J). The orlistat–treated group showed remarkable reduction in the density of vacuolated hepatocytes, and other injuries compared to the HCD group (Figure 3K and 3L). The observed histopathological changes in various experimental groups were scored in at least 3 slides for each individual animal, and the final score was calculated (Table 4).


Hawthorn ethanolic extracts with triterpenoids and flavonoids exert hepatoprotective effects and suppress the hypercholesterolemia-induced oxidative stress in rats.

Rezaei-Golmisheh A, Malekinejad H, Asri-Rezaei S, Farshid AA, Akbari P - Iran J Basic Med Sci (2015)

Histopathological findings of rat livers from different treatment groups. (A and B): Control, (C and D): hypercholesterolemic group (HC), (E and F): T1, (G and H): T2, (I and J): T3, (K and L): T4. a: congestion, b: bile ducts hyperplasia, c: inflammatory cells infiltration, d: vacoulation and e: pyknotic nuclei. [H&E staining; (A, C, E, G, I and K ×100) and (B, D, F, H, J and L ×400)]
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4556763&req=5

Figure 3: Histopathological findings of rat livers from different treatment groups. (A and B): Control, (C and D): hypercholesterolemic group (HC), (E and F): T1, (G and H): T2, (I and J): T3, (K and L): T4. a: congestion, b: bile ducts hyperplasia, c: inflammatory cells infiltration, d: vacoulation and e: pyknotic nuclei. [H&E staining; (A, C, E, G, I and K ×100) and (B, D, F, H, J and L ×400)]
Mentions: The normal histological feature of the liver from the control (C) rats are depicted in Figure 3-A and 3-B. Histopathological findings in the liver of HC animals represented congested blood vessels, bile duct hyperplasia, inflammatory cells infiltration (perivascular cuffing), highly vacuolated hepatocytes and pyknotic nuclei (Figure 3C, and 3D). Administration of bark and leaves extracts although reduced the HCD-induced damages in the liver, there are however symptoms of congestion, perivascular cuffing and vacuolated hepatocytes (Figure 3E, 3F, 3G and 3H, respectively). We found that 4 weeks administration of hawthorn berries extract could fairly improve the HCD-induced hepatic damages, and the liver looks normal (Figure 3-I and 3-J). The orlistat–treated group showed remarkable reduction in the density of vacuolated hepatocytes, and other injuries compared to the HCD group (Figure 3K and 3L). The observed histopathological changes in various experimental groups were scored in at least 3 slides for each individual animal, and the final score was calculated (Table 4).

Bottom Line: The highest phenol content, oleanolic acid, quercetin and lupeol levels and free radical scavenging potency were found in the bark extract, and the highest ursolic acid level was found in the berries extract.Hawthorn's extracts protected from hepatic thiol depletion and improved the lipid profile and hepatic damages.Moreover, the hypocholesterolemic effect of extracts was found comparable to orlistat.

View Article: PubMed Central - PubMed

Affiliation: Department of Histology and Embryology, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran.

ABSTRACT

Objectives: The current study was aimed to determine the bioactive constituents and biological effects of the Crataegus monogyna ethanolic extracts from bark, leaves and berries on hypercholesterolemia.

Materials and methods: Oleanolic acid, ursolic acid, quercetin and lupeol concentrations were quantified by HPLC. Total phenol content and radical scavenging activity of extracts were also measured. The hypocholesterolemic, antioxidant, and hepatoprotective effects of the extracts were examined in hypercholesterolemic rats and compared with orlistat.

Results: The highest phenol content, oleanolic acid, quercetin and lupeol levels and free radical scavenging potency were found in the bark extract, and the highest ursolic acid level was found in the berries extract. Orlistat and extracts significantly (P<0.05) lowered the hypercholesterolemia-increased serum level of hepatic enzymes and lipid peroxidation level. Hawthorn's extracts protected from hepatic thiol depletion and improved the lipid profile and hepatic damages.

Conclusion: Data suggested that hawthorn's extracts are able to protect from hypercholesterolemia-induced oxidative stress and hepatic injuries. Moreover, the hypocholesterolemic effect of extracts was found comparable to orlistat.

No MeSH data available.


Related in: MedlinePlus