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Ultraviolet B efficacy in improving antileishmanial effects of silver nanoparticles.

Mayelifar K, Taheri AR, Rajabi O, Sazgarnia A - Iran J Basic Med Sci (2015)

Bottom Line: The results showed the highest pronounced inhibitory effect in the group receiving AgNPs plus UVB.In addition, a significant difference was obtained between the group receiving AgNPs alone and the one with combinational therapy.It could be suggested that UVB in the presence of AgNPs, by inhibiting the spread of CL lesions and reducing the rate of visceral progression of the disease, provides a serious anti-leishmanial effect.

View Article: PubMed Central - PubMed

Affiliation: Department of Medical Physics, Hormozgan University of Medical Sciences, Bandar Abbas, Iran.

ABSTRACT

Objectives: Cutaneous Leishmaniasis (CL) is a parasitic disease caused by various species of the flagellated protozoan, Leishmania. Regardless of the numerous studies, there are still serious challenges in the treatment of CL. This study aimed at evaluating the influence of a low dose ultraviolet B (UVB) radiation along with silver nanoparticles (AgNPs) on a mouse model of CL induced by Leishmania major.

Materials and methods: L. major promastigotes (MRHO/IR/75/ER) were extracted from infected mice spleens. Two months after subcutaneous injection of 2×10(6) promastigotes into the footpad of BALB/c mice, when the lesions were developed, the animals were divided into 4 groups including one control group and three study groups: AgNPs, UVB and UVB plus AgNPs. Spleen parasite burden was assessed on day 40 after the first treatment. The data were analyzed by Instat, Elida and SPSS 16 software programs.

Results: The results showed the highest pronounced inhibitory effect in the group receiving AgNPs plus UVB. In addition, a significant difference was obtained between the group receiving AgNPs alone and the one with combinational therapy. The findings on parasite burden showed a significant difference between the control group and other treatment groups.

Conclusion: It could be suggested that UVB in the presence of AgNPs, by inhibiting the spread of CL lesions and reducing the rate of visceral progression of the disease, provides a serious anti-leishmanial effect.

No MeSH data available.


Related in: MedlinePlus

Three images taken from the ulcers on the mice’s footpad belonged to various treatment groups: a. treated by AgNPs, b. irradiated by UVB, c. UVB irradiated with AgNPs
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Figure 5: Three images taken from the ulcers on the mice’s footpad belonged to various treatment groups: a. treated by AgNPs, b. irradiated by UVB, c. UVB irradiated with AgNPs

Mentions: We also had some qualitative observations. As shown in Figure 5, the scaling of the lesions began in the third and fourth treatment sessions in the groups treated with UVB radiation alone and UVB radiation in combination with AgNPs, which could be a sign of healing.


Ultraviolet B efficacy in improving antileishmanial effects of silver nanoparticles.

Mayelifar K, Taheri AR, Rajabi O, Sazgarnia A - Iran J Basic Med Sci (2015)

Three images taken from the ulcers on the mice’s footpad belonged to various treatment groups: a. treated by AgNPs, b. irradiated by UVB, c. UVB irradiated with AgNPs
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4556761&req=5

Figure 5: Three images taken from the ulcers on the mice’s footpad belonged to various treatment groups: a. treated by AgNPs, b. irradiated by UVB, c. UVB irradiated with AgNPs
Mentions: We also had some qualitative observations. As shown in Figure 5, the scaling of the lesions began in the third and fourth treatment sessions in the groups treated with UVB radiation alone and UVB radiation in combination with AgNPs, which could be a sign of healing.

Bottom Line: The results showed the highest pronounced inhibitory effect in the group receiving AgNPs plus UVB.In addition, a significant difference was obtained between the group receiving AgNPs alone and the one with combinational therapy.It could be suggested that UVB in the presence of AgNPs, by inhibiting the spread of CL lesions and reducing the rate of visceral progression of the disease, provides a serious anti-leishmanial effect.

View Article: PubMed Central - PubMed

Affiliation: Department of Medical Physics, Hormozgan University of Medical Sciences, Bandar Abbas, Iran.

ABSTRACT

Objectives: Cutaneous Leishmaniasis (CL) is a parasitic disease caused by various species of the flagellated protozoan, Leishmania. Regardless of the numerous studies, there are still serious challenges in the treatment of CL. This study aimed at evaluating the influence of a low dose ultraviolet B (UVB) radiation along with silver nanoparticles (AgNPs) on a mouse model of CL induced by Leishmania major.

Materials and methods: L. major promastigotes (MRHO/IR/75/ER) were extracted from infected mice spleens. Two months after subcutaneous injection of 2×10(6) promastigotes into the footpad of BALB/c mice, when the lesions were developed, the animals were divided into 4 groups including one control group and three study groups: AgNPs, UVB and UVB plus AgNPs. Spleen parasite burden was assessed on day 40 after the first treatment. The data were analyzed by Instat, Elida and SPSS 16 software programs.

Results: The results showed the highest pronounced inhibitory effect in the group receiving AgNPs plus UVB. In addition, a significant difference was obtained between the group receiving AgNPs alone and the one with combinational therapy. The findings on parasite burden showed a significant difference between the control group and other treatment groups.

Conclusion: It could be suggested that UVB in the presence of AgNPs, by inhibiting the spread of CL lesions and reducing the rate of visceral progression of the disease, provides a serious anti-leishmanial effect.

No MeSH data available.


Related in: MedlinePlus