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Calcitriol modulates the effects of bone marrow-derived mesenchymal stem cells on macrophage functions.

Motlagh BM, Ahangaran NA, Froushani SM - Iran J Basic Med Sci (2015)

Bottom Line: Some evidence showed that calcitriol has an important role in regulating growth and differentiation of mesenchymal stem cells (MSCs).MSCs were isolated from rat bone marrow and pulsed with different concentrations of calcitriol (50, 100 and 200 nanomolar) for 24, 48 and 72 hr.Our data showed that bone marrow-derived MSCs pulsed with calcitriol may cause a significant increase in uptake of neutral red and phagocytic activity of opsonized heat killed baker's yeast.

View Article: PubMed Central - PubMed

Affiliation: Department of Microbiology, Veterinary Faculty, Urmia University, Urmia, Iran.

ABSTRACT

Objectives: Some evidence showed that calcitriol has an important role in regulating growth and differentiation of mesenchymal stem cells (MSCs). However, the interaction between mesenchymal stem cells and macrophage is not clear yet. The current study was done to investigate the in vitro effects of calcitriol on the interactions between bone marrow-derived MSCs and rat macrophages.

Materials and methods: MSCs were isolated from rat bone marrow and pulsed with different concentrations of calcitriol (50, 100 and 200 nanomolar) for 24, 48 and 72 hr. Then, mesenchymal stem cells were co-cultured with macrophages for 4 hr. Finally, macrophages were evaluated for ability to uptake neutral red, phagocytosis activity against opsonized yeast, respiratory burst and viability.

Results: Our data showed that bone marrow-derived MSCs pulsed with calcitriol may cause a significant increase in uptake of neutral red and phagocytic activity of opsonized heat killed baker's yeast. Moreover, treatment of MSCs with calcitriol enhanced macrophage viability. Nevertheless, the respiratory burst of macrophages was significantly reduced in macrophages co-cultured with calcitriol-treated MSCs compared to control group.

Conclusion: Calcitriol may accelerate and potentiate anti-inflammatory M2 macrophage polarization by MSCs.

No MeSH data available.


Related in: MedlinePlus

Effect of calcitriol on modulation of macrophage viability by MSCs. As compared to control group, calcitriol-treated MSCs significantly increased the viability of macrophage in a dose-dependent manner, (*P< 0. 01, ** P< 0. 001 vs control group)
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Figure 4: Effect of calcitriol on modulation of macrophage viability by MSCs. As compared to control group, calcitriol-treated MSCs significantly increased the viability of macrophage in a dose-dependent manner, (*P< 0. 01, ** P< 0. 001 vs control group)

Mentions: Macrophage metabolic activity that was measured by MTT assay was used to evaluate survivability of macrophages. Our findings indicated that, in a dose-dependent manner, the survival rate of macrophages that were co-cultured with calcitriol-treated MSCs, was significantly greater than that of macrophages co-cultured with non-treated MSCs (Figure 4).


Calcitriol modulates the effects of bone marrow-derived mesenchymal stem cells on macrophage functions.

Motlagh BM, Ahangaran NA, Froushani SM - Iran J Basic Med Sci (2015)

Effect of calcitriol on modulation of macrophage viability by MSCs. As compared to control group, calcitriol-treated MSCs significantly increased the viability of macrophage in a dose-dependent manner, (*P< 0. 01, ** P< 0. 001 vs control group)
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4556760&req=5

Figure 4: Effect of calcitriol on modulation of macrophage viability by MSCs. As compared to control group, calcitriol-treated MSCs significantly increased the viability of macrophage in a dose-dependent manner, (*P< 0. 01, ** P< 0. 001 vs control group)
Mentions: Macrophage metabolic activity that was measured by MTT assay was used to evaluate survivability of macrophages. Our findings indicated that, in a dose-dependent manner, the survival rate of macrophages that were co-cultured with calcitriol-treated MSCs, was significantly greater than that of macrophages co-cultured with non-treated MSCs (Figure 4).

Bottom Line: Some evidence showed that calcitriol has an important role in regulating growth and differentiation of mesenchymal stem cells (MSCs).MSCs were isolated from rat bone marrow and pulsed with different concentrations of calcitriol (50, 100 and 200 nanomolar) for 24, 48 and 72 hr.Our data showed that bone marrow-derived MSCs pulsed with calcitriol may cause a significant increase in uptake of neutral red and phagocytic activity of opsonized heat killed baker's yeast.

View Article: PubMed Central - PubMed

Affiliation: Department of Microbiology, Veterinary Faculty, Urmia University, Urmia, Iran.

ABSTRACT

Objectives: Some evidence showed that calcitriol has an important role in regulating growth and differentiation of mesenchymal stem cells (MSCs). However, the interaction between mesenchymal stem cells and macrophage is not clear yet. The current study was done to investigate the in vitro effects of calcitriol on the interactions between bone marrow-derived MSCs and rat macrophages.

Materials and methods: MSCs were isolated from rat bone marrow and pulsed with different concentrations of calcitriol (50, 100 and 200 nanomolar) for 24, 48 and 72 hr. Then, mesenchymal stem cells were co-cultured with macrophages for 4 hr. Finally, macrophages were evaluated for ability to uptake neutral red, phagocytosis activity against opsonized yeast, respiratory burst and viability.

Results: Our data showed that bone marrow-derived MSCs pulsed with calcitriol may cause a significant increase in uptake of neutral red and phagocytic activity of opsonized heat killed baker's yeast. Moreover, treatment of MSCs with calcitriol enhanced macrophage viability. Nevertheless, the respiratory burst of macrophages was significantly reduced in macrophages co-cultured with calcitriol-treated MSCs compared to control group.

Conclusion: Calcitriol may accelerate and potentiate anti-inflammatory M2 macrophage polarization by MSCs.

No MeSH data available.


Related in: MedlinePlus