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Calcitriol modulates the effects of bone marrow-derived mesenchymal stem cells on macrophage functions.

Motlagh BM, Ahangaran NA, Froushani SM - Iran J Basic Med Sci (2015)

Bottom Line: Some evidence showed that calcitriol has an important role in regulating growth and differentiation of mesenchymal stem cells (MSCs).MSCs were isolated from rat bone marrow and pulsed with different concentrations of calcitriol (50, 100 and 200 nanomolar) for 24, 48 and 72 hr.Our data showed that bone marrow-derived MSCs pulsed with calcitriol may cause a significant increase in uptake of neutral red and phagocytic activity of opsonized heat killed baker's yeast.

View Article: PubMed Central - PubMed

Affiliation: Department of Microbiology, Veterinary Faculty, Urmia University, Urmia, Iran.

ABSTRACT

Objectives: Some evidence showed that calcitriol has an important role in regulating growth and differentiation of mesenchymal stem cells (MSCs). However, the interaction between mesenchymal stem cells and macrophage is not clear yet. The current study was done to investigate the in vitro effects of calcitriol on the interactions between bone marrow-derived MSCs and rat macrophages.

Materials and methods: MSCs were isolated from rat bone marrow and pulsed with different concentrations of calcitriol (50, 100 and 200 nanomolar) for 24, 48 and 72 hr. Then, mesenchymal stem cells were co-cultured with macrophages for 4 hr. Finally, macrophages were evaluated for ability to uptake neutral red, phagocytosis activity against opsonized yeast, respiratory burst and viability.

Results: Our data showed that bone marrow-derived MSCs pulsed with calcitriol may cause a significant increase in uptake of neutral red and phagocytic activity of opsonized heat killed baker's yeast. Moreover, treatment of MSCs with calcitriol enhanced macrophage viability. Nevertheless, the respiratory burst of macrophages was significantly reduced in macrophages co-cultured with calcitriol-treated MSCs compared to control group.

Conclusion: Calcitriol may accelerate and potentiate anti-inflammatory M2 macrophage polarization by MSCs.

No MeSH data available.


Related in: MedlinePlus

Phagocytic activity of macrophages that were co-cultured with MSCs against opsonized yeast Compared to control group, calcitriol-treated MSCs significantly increased phagocytic activity of macrophages (* P< 0.01, ** P< 0. 001 vs control group)
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Figure 2: Phagocytic activity of macrophages that were co-cultured with MSCs against opsonized yeast Compared to control group, calcitriol-treated MSCs significantly increased phagocytic activity of macrophages (* P< 0.01, ** P< 0. 001 vs control group)

Mentions: Phagocytosis is an essential function of macrophages (20). Neutral red (NR) is a vital stain that is engulfed by viable macrophages and accumulated in their lysosomes (21). The data showed that following the co-culture with calcitriol- treated MSCs, opsonin independent phagocytosis of macrophages was increased significantly in a dose-dependent manner (Figure 1). As shown in Figure 2, opsonin dependent phagocytosis of macrophages that were co-cultured with calcitriol-treated MSCs, was significantly increased. However, this change was not in a dose-dependent manner.


Calcitriol modulates the effects of bone marrow-derived mesenchymal stem cells on macrophage functions.

Motlagh BM, Ahangaran NA, Froushani SM - Iran J Basic Med Sci (2015)

Phagocytic activity of macrophages that were co-cultured with MSCs against opsonized yeast Compared to control group, calcitriol-treated MSCs significantly increased phagocytic activity of macrophages (* P< 0.01, ** P< 0. 001 vs control group)
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4556760&req=5

Figure 2: Phagocytic activity of macrophages that were co-cultured with MSCs against opsonized yeast Compared to control group, calcitriol-treated MSCs significantly increased phagocytic activity of macrophages (* P< 0.01, ** P< 0. 001 vs control group)
Mentions: Phagocytosis is an essential function of macrophages (20). Neutral red (NR) is a vital stain that is engulfed by viable macrophages and accumulated in their lysosomes (21). The data showed that following the co-culture with calcitriol- treated MSCs, opsonin independent phagocytosis of macrophages was increased significantly in a dose-dependent manner (Figure 1). As shown in Figure 2, opsonin dependent phagocytosis of macrophages that were co-cultured with calcitriol-treated MSCs, was significantly increased. However, this change was not in a dose-dependent manner.

Bottom Line: Some evidence showed that calcitriol has an important role in regulating growth and differentiation of mesenchymal stem cells (MSCs).MSCs were isolated from rat bone marrow and pulsed with different concentrations of calcitriol (50, 100 and 200 nanomolar) for 24, 48 and 72 hr.Our data showed that bone marrow-derived MSCs pulsed with calcitriol may cause a significant increase in uptake of neutral red and phagocytic activity of opsonized heat killed baker's yeast.

View Article: PubMed Central - PubMed

Affiliation: Department of Microbiology, Veterinary Faculty, Urmia University, Urmia, Iran.

ABSTRACT

Objectives: Some evidence showed that calcitriol has an important role in regulating growth and differentiation of mesenchymal stem cells (MSCs). However, the interaction between mesenchymal stem cells and macrophage is not clear yet. The current study was done to investigate the in vitro effects of calcitriol on the interactions between bone marrow-derived MSCs and rat macrophages.

Materials and methods: MSCs were isolated from rat bone marrow and pulsed with different concentrations of calcitriol (50, 100 and 200 nanomolar) for 24, 48 and 72 hr. Then, mesenchymal stem cells were co-cultured with macrophages for 4 hr. Finally, macrophages were evaluated for ability to uptake neutral red, phagocytosis activity against opsonized yeast, respiratory burst and viability.

Results: Our data showed that bone marrow-derived MSCs pulsed with calcitriol may cause a significant increase in uptake of neutral red and phagocytic activity of opsonized heat killed baker's yeast. Moreover, treatment of MSCs with calcitriol enhanced macrophage viability. Nevertheless, the respiratory burst of macrophages was significantly reduced in macrophages co-cultured with calcitriol-treated MSCs compared to control group.

Conclusion: Calcitriol may accelerate and potentiate anti-inflammatory M2 macrophage polarization by MSCs.

No MeSH data available.


Related in: MedlinePlus