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The effect of silver nanoparticles on apoptosis and dark neuron production in rat hippocampus.

Bagheri-Abassi F, Alavi H, Mohammadipour A, Motejaded F, Ebrahimzadeh-Bideskan A - Iran J Basic Med Sci (2015)

Bottom Line: Then to compare the groups, dark neurons (DNs) and apoptotic neurons were counted by morphometric method.Results showed that the numbers of DNs and apoptotic cells in the CA1, CA2, CA3, and dentate gyrus (DG) of hippocampus significantly increased in the Ag-NPs group in comparison to the control group (P<0.05).Exposure to Ag-NPs can induce dark neuron and apoptotic cells in the hippocampus.

View Article: PubMed Central - PubMed

Affiliation: Department of Anatomy and Cell Biology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.

ABSTRACT

Objectives: Silver nanoparticles (Ag-NPs) are used widely in bedding, water purification, tooth paste and toys. These nanoparticles can enter into the body and move into the hippocampus. The aim of this study was to investigate the neurotoxicity of silver nanoparticles in the adult rat hippocampus.

Materials and methods: 12 male Wistar rats were randomly divided into two experimental and control groups (6 rats in each group). Animals in the experimental group received Ag-NPs (30 mg/kg) orally (gavage) for 28 consecutive days. Control group in the same period was treated with distilled water via gavage. At the end of experiment, animals were deeply anesthetized, sacrificed, and their brains were collected from each group. Finally the brain sections were stained using toluidine blue and TUNEL. Then to compare the groups, dark neurons (DNs) and apoptotic neurons were counted by morphometric method.

Results: Results showed that the numbers of DNs and apoptotic cells in the CA1, CA2, CA3, and dentate gyrus (DG) of hippocampus significantly increased in the Ag-NPs group in comparison to the control group (P<0.05).

Conclusion: Exposure to Ag-NPs can induce dark neuron and apoptotic cells in the hippocampus.

No MeSH data available.


Related in: MedlinePlus

Photomicrographs of the adult rat hippocampus, Exposure to Ag-NPs increased apoptotic cells in the hippocampus. (A) CA1 Control group, (B) CA1 Ag-NPs group, (C) CA2 Control group, (D) CA2 Ag-NPs group, (E) CA3 Control group, (F) CA3 Ag-NPs group, (G) DG Control group, (H) DG Ag-NPs group, Arrow heads point to representative TUNEL positive cells with dark brown nucleus. Scale bars, 150 µm
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Figure 3: Photomicrographs of the adult rat hippocampus, Exposure to Ag-NPs increased apoptotic cells in the hippocampus. (A) CA1 Control group, (B) CA1 Ag-NPs group, (C) CA2 Control group, (D) CA2 Ag-NPs group, (E) CA3 Control group, (F) CA3 Ag-NPs group, (G) DG Control group, (H) DG Ag-NPs group, Arrow heads point to representative TUNEL positive cells with dark brown nucleus. Scale bars, 150 µm

Mentions: The numbers of TUNEL positive cells per unit area (NA) of the CA1, CA2, CA3 and DG subdivisions of the hippocampus were counted. In comparison to control group, the mean of TUNEL positive cells number per unit area in CA1, CA2, CA3 and DG of hippocampus was increased significantly in Ag-NPs group (P<0.01), (Figures 3 and 4).


The effect of silver nanoparticles on apoptosis and dark neuron production in rat hippocampus.

Bagheri-Abassi F, Alavi H, Mohammadipour A, Motejaded F, Ebrahimzadeh-Bideskan A - Iran J Basic Med Sci (2015)

Photomicrographs of the adult rat hippocampus, Exposure to Ag-NPs increased apoptotic cells in the hippocampus. (A) CA1 Control group, (B) CA1 Ag-NPs group, (C) CA2 Control group, (D) CA2 Ag-NPs group, (E) CA3 Control group, (F) CA3 Ag-NPs group, (G) DG Control group, (H) DG Ag-NPs group, Arrow heads point to representative TUNEL positive cells with dark brown nucleus. Scale bars, 150 µm
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4556755&req=5

Figure 3: Photomicrographs of the adult rat hippocampus, Exposure to Ag-NPs increased apoptotic cells in the hippocampus. (A) CA1 Control group, (B) CA1 Ag-NPs group, (C) CA2 Control group, (D) CA2 Ag-NPs group, (E) CA3 Control group, (F) CA3 Ag-NPs group, (G) DG Control group, (H) DG Ag-NPs group, Arrow heads point to representative TUNEL positive cells with dark brown nucleus. Scale bars, 150 µm
Mentions: The numbers of TUNEL positive cells per unit area (NA) of the CA1, CA2, CA3 and DG subdivisions of the hippocampus were counted. In comparison to control group, the mean of TUNEL positive cells number per unit area in CA1, CA2, CA3 and DG of hippocampus was increased significantly in Ag-NPs group (P<0.01), (Figures 3 and 4).

Bottom Line: Then to compare the groups, dark neurons (DNs) and apoptotic neurons were counted by morphometric method.Results showed that the numbers of DNs and apoptotic cells in the CA1, CA2, CA3, and dentate gyrus (DG) of hippocampus significantly increased in the Ag-NPs group in comparison to the control group (P<0.05).Exposure to Ag-NPs can induce dark neuron and apoptotic cells in the hippocampus.

View Article: PubMed Central - PubMed

Affiliation: Department of Anatomy and Cell Biology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.

ABSTRACT

Objectives: Silver nanoparticles (Ag-NPs) are used widely in bedding, water purification, tooth paste and toys. These nanoparticles can enter into the body and move into the hippocampus. The aim of this study was to investigate the neurotoxicity of silver nanoparticles in the adult rat hippocampus.

Materials and methods: 12 male Wistar rats were randomly divided into two experimental and control groups (6 rats in each group). Animals in the experimental group received Ag-NPs (30 mg/kg) orally (gavage) for 28 consecutive days. Control group in the same period was treated with distilled water via gavage. At the end of experiment, animals were deeply anesthetized, sacrificed, and their brains were collected from each group. Finally the brain sections were stained using toluidine blue and TUNEL. Then to compare the groups, dark neurons (DNs) and apoptotic neurons were counted by morphometric method.

Results: Results showed that the numbers of DNs and apoptotic cells in the CA1, CA2, CA3, and dentate gyrus (DG) of hippocampus significantly increased in the Ag-NPs group in comparison to the control group (P<0.05).

Conclusion: Exposure to Ag-NPs can induce dark neuron and apoptotic cells in the hippocampus.

No MeSH data available.


Related in: MedlinePlus