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The effect of silver nanoparticles on apoptosis and dark neuron production in rat hippocampus.

Bagheri-Abassi F, Alavi H, Mohammadipour A, Motejaded F, Ebrahimzadeh-Bideskan A - Iran J Basic Med Sci (2015)

Bottom Line: Then to compare the groups, dark neurons (DNs) and apoptotic neurons were counted by morphometric method.Results showed that the numbers of DNs and apoptotic cells in the CA1, CA2, CA3, and dentate gyrus (DG) of hippocampus significantly increased in the Ag-NPs group in comparison to the control group (P<0.05).Exposure to Ag-NPs can induce dark neuron and apoptotic cells in the hippocampus.

View Article: PubMed Central - PubMed

Affiliation: Department of Anatomy and Cell Biology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.

ABSTRACT

Objectives: Silver nanoparticles (Ag-NPs) are used widely in bedding, water purification, tooth paste and toys. These nanoparticles can enter into the body and move into the hippocampus. The aim of this study was to investigate the neurotoxicity of silver nanoparticles in the adult rat hippocampus.

Materials and methods: 12 male Wistar rats were randomly divided into two experimental and control groups (6 rats in each group). Animals in the experimental group received Ag-NPs (30 mg/kg) orally (gavage) for 28 consecutive days. Control group in the same period was treated with distilled water via gavage. At the end of experiment, animals were deeply anesthetized, sacrificed, and their brains were collected from each group. Finally the brain sections were stained using toluidine blue and TUNEL. Then to compare the groups, dark neurons (DNs) and apoptotic neurons were counted by morphometric method.

Results: Results showed that the numbers of DNs and apoptotic cells in the CA1, CA2, CA3, and dentate gyrus (DG) of hippocampus significantly increased in the Ag-NPs group in comparison to the control group (P<0.05).

Conclusion: Exposure to Ag-NPs can induce dark neuron and apoptotic cells in the hippocampus.

No MeSH data available.


Related in: MedlinePlus

Ag-NPs exposure for 28 days induces DNs production in the adult rat hippocampus. Bars marked with an asterisk or double asterisks mean that it is significantly different from the control at the 5% or 1% confidence level, respectively. The data are presented as the mean ± SEM
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Figure 2: Ag-NPs exposure for 28 days induces DNs production in the adult rat hippocampus. Bars marked with an asterisk or double asterisks mean that it is significantly different from the control at the 5% or 1% confidence level, respectively. The data are presented as the mean ± SEM

Mentions: The numbers of DNs per unit area (NA) of the CA1, CA2, CA3 and DG subdivisions of the hippocampus were counted. Then the mean number of DNs in the hippocampus of experimental group compared with the control group. A few DNs were found in different regions of hippocampus in control group. In comparison to control group, the mean of DNs number per unit area in CA1, CA2, CA3 and DG of hippocampus was significantly increased in Ag-NPs group (P<0.05 for CA1 and P<0.01 for other areas) (Figure 1, 2).


The effect of silver nanoparticles on apoptosis and dark neuron production in rat hippocampus.

Bagheri-Abassi F, Alavi H, Mohammadipour A, Motejaded F, Ebrahimzadeh-Bideskan A - Iran J Basic Med Sci (2015)

Ag-NPs exposure for 28 days induces DNs production in the adult rat hippocampus. Bars marked with an asterisk or double asterisks mean that it is significantly different from the control at the 5% or 1% confidence level, respectively. The data are presented as the mean ± SEM
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4556755&req=5

Figure 2: Ag-NPs exposure for 28 days induces DNs production in the adult rat hippocampus. Bars marked with an asterisk or double asterisks mean that it is significantly different from the control at the 5% or 1% confidence level, respectively. The data are presented as the mean ± SEM
Mentions: The numbers of DNs per unit area (NA) of the CA1, CA2, CA3 and DG subdivisions of the hippocampus were counted. Then the mean number of DNs in the hippocampus of experimental group compared with the control group. A few DNs were found in different regions of hippocampus in control group. In comparison to control group, the mean of DNs number per unit area in CA1, CA2, CA3 and DG of hippocampus was significantly increased in Ag-NPs group (P<0.05 for CA1 and P<0.01 for other areas) (Figure 1, 2).

Bottom Line: Then to compare the groups, dark neurons (DNs) and apoptotic neurons were counted by morphometric method.Results showed that the numbers of DNs and apoptotic cells in the CA1, CA2, CA3, and dentate gyrus (DG) of hippocampus significantly increased in the Ag-NPs group in comparison to the control group (P<0.05).Exposure to Ag-NPs can induce dark neuron and apoptotic cells in the hippocampus.

View Article: PubMed Central - PubMed

Affiliation: Department of Anatomy and Cell Biology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.

ABSTRACT

Objectives: Silver nanoparticles (Ag-NPs) are used widely in bedding, water purification, tooth paste and toys. These nanoparticles can enter into the body and move into the hippocampus. The aim of this study was to investigate the neurotoxicity of silver nanoparticles in the adult rat hippocampus.

Materials and methods: 12 male Wistar rats were randomly divided into two experimental and control groups (6 rats in each group). Animals in the experimental group received Ag-NPs (30 mg/kg) orally (gavage) for 28 consecutive days. Control group in the same period was treated with distilled water via gavage. At the end of experiment, animals were deeply anesthetized, sacrificed, and their brains were collected from each group. Finally the brain sections were stained using toluidine blue and TUNEL. Then to compare the groups, dark neurons (DNs) and apoptotic neurons were counted by morphometric method.

Results: Results showed that the numbers of DNs and apoptotic cells in the CA1, CA2, CA3, and dentate gyrus (DG) of hippocampus significantly increased in the Ag-NPs group in comparison to the control group (P<0.05).

Conclusion: Exposure to Ag-NPs can induce dark neuron and apoptotic cells in the hippocampus.

No MeSH data available.


Related in: MedlinePlus