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Genomic and Phenomic Study of Mammary Pathogenic Escherichia coli.

Blum SE, Heller ED, Sela S, Elad D, Edery N, Leitner G - PLoS ONE (2015)

Bottom Line: Analysis of whole genome sequences and predicted proteomes revealed high similarity among MPEC, whereas MPEC significantly differed from the non-mammary pathogenic strain K71, and from E. coli genomes from other pathotypes.Functional features identified in MPEC genomes and lacking in the non-mammary pathogenic strain were associated with synthesis of lipopolysaccharide and other membrane antigens, ferric-dicitrate iron acquisition and sugars metabolism.Among phenotypes unique to MPEC compared to the non-mammary pathogenic strain were uric acid and D-serine metabolism.

View Article: PubMed Central - PubMed

Affiliation: Department of Animal Sciences, Robert H. Smith Faculty of Agriculture, Food and Environment, Rehovot, Israel; National Mastitis Reference Center, Department of Bacteriology, Kimron Veterinary Institute, Bet Dagan, Israel; Department of Bacteriology, Kimron Veterinary Institute, Bet Dagan, Israel.

ABSTRACT
Escherichia coli is a major etiological agent of intra-mammary infections (IMI) in cows, leading to acute mastitis and causing great economic losses in dairy production worldwide. Particular strains cause persistent IMI, leading to recurrent mastitis. Virulence factors of mammary pathogenic E. coli (MPEC) involved pathogenesis of mastitis as well as those differentiating strains causing acute or persistent mastitis are largely unknown. This study aimed to identify virulence markers in MPEC through whole genome and phenome comparative analysis. MPEC strains causing acute (VL2874 and P4) or persistent (VL2732) mastitis were compared to an environmental strain (K71) and to the genomes of strains representing different E. coli pathotypes. Intra-mammary challenge in mice confirmed experimentally that the strains studied here have different pathogenic potential, and that the environmental strain K71 is non-pathogenic in the mammary gland. Analysis of whole genome sequences and predicted proteomes revealed high similarity among MPEC, whereas MPEC significantly differed from the non-mammary pathogenic strain K71, and from E. coli genomes from other pathotypes. Functional features identified in MPEC genomes and lacking in the non-mammary pathogenic strain were associated with synthesis of lipopolysaccharide and other membrane antigens, ferric-dicitrate iron acquisition and sugars metabolism. Features associated with cytotoxicity or intra-cellular survival were found specifically in the genomes of strains from severe and acute (VL2874) or persistent (VL2732) mastitis, respectively. MPEC genomes were relatively similar to strain K-12, which was subsequently shown here to be possibly pathogenic in the mammary gland. Phenome analysis showed that the persistent MPEC was the most versatile in terms of nutrients metabolized and acute MPEC the least. Among phenotypes unique to MPEC compared to the non-mammary pathogenic strain were uric acid and D-serine metabolism. This study reveals virulence factors and phenotypic characteristics of MPEC that may play a role in pathogenesis of E. coli mastitis.

No MeSH data available.


Related in: MedlinePlus

Distribution of functional annotation into RAST subsystems.
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pone.0136387.g003: Distribution of functional annotation into RAST subsystems.

Mentions: Overall, the four genomes showed a similar distribution of features into subsystem categories using the RAST subsystems annotation (Fig 3). Differences were found notably in mobile elements (phages, plasmids), metabolism of aromatic compounds, membrane transport and lipid metabolism categories. Many elements belonging to metabolism of aromatic compounds were lacking in strain VL2732. Differences in the membrane transport subsystem were attributed to features belonging to type IV secretion system found in strains K71 and P4, and to type VI secretion system found in strains K71 and VL2874. Differences in the lipid metabolism subsystem were attributed to a phospholipid and fatty acid biosynthesis related cluster that is present only in strain P4.


Genomic and Phenomic Study of Mammary Pathogenic Escherichia coli.

Blum SE, Heller ED, Sela S, Elad D, Edery N, Leitner G - PLoS ONE (2015)

Distribution of functional annotation into RAST subsystems.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4556653&req=5

pone.0136387.g003: Distribution of functional annotation into RAST subsystems.
Mentions: Overall, the four genomes showed a similar distribution of features into subsystem categories using the RAST subsystems annotation (Fig 3). Differences were found notably in mobile elements (phages, plasmids), metabolism of aromatic compounds, membrane transport and lipid metabolism categories. Many elements belonging to metabolism of aromatic compounds were lacking in strain VL2732. Differences in the membrane transport subsystem were attributed to features belonging to type IV secretion system found in strains K71 and P4, and to type VI secretion system found in strains K71 and VL2874. Differences in the lipid metabolism subsystem were attributed to a phospholipid and fatty acid biosynthesis related cluster that is present only in strain P4.

Bottom Line: Analysis of whole genome sequences and predicted proteomes revealed high similarity among MPEC, whereas MPEC significantly differed from the non-mammary pathogenic strain K71, and from E. coli genomes from other pathotypes.Functional features identified in MPEC genomes and lacking in the non-mammary pathogenic strain were associated with synthesis of lipopolysaccharide and other membrane antigens, ferric-dicitrate iron acquisition and sugars metabolism.Among phenotypes unique to MPEC compared to the non-mammary pathogenic strain were uric acid and D-serine metabolism.

View Article: PubMed Central - PubMed

Affiliation: Department of Animal Sciences, Robert H. Smith Faculty of Agriculture, Food and Environment, Rehovot, Israel; National Mastitis Reference Center, Department of Bacteriology, Kimron Veterinary Institute, Bet Dagan, Israel; Department of Bacteriology, Kimron Veterinary Institute, Bet Dagan, Israel.

ABSTRACT
Escherichia coli is a major etiological agent of intra-mammary infections (IMI) in cows, leading to acute mastitis and causing great economic losses in dairy production worldwide. Particular strains cause persistent IMI, leading to recurrent mastitis. Virulence factors of mammary pathogenic E. coli (MPEC) involved pathogenesis of mastitis as well as those differentiating strains causing acute or persistent mastitis are largely unknown. This study aimed to identify virulence markers in MPEC through whole genome and phenome comparative analysis. MPEC strains causing acute (VL2874 and P4) or persistent (VL2732) mastitis were compared to an environmental strain (K71) and to the genomes of strains representing different E. coli pathotypes. Intra-mammary challenge in mice confirmed experimentally that the strains studied here have different pathogenic potential, and that the environmental strain K71 is non-pathogenic in the mammary gland. Analysis of whole genome sequences and predicted proteomes revealed high similarity among MPEC, whereas MPEC significantly differed from the non-mammary pathogenic strain K71, and from E. coli genomes from other pathotypes. Functional features identified in MPEC genomes and lacking in the non-mammary pathogenic strain were associated with synthesis of lipopolysaccharide and other membrane antigens, ferric-dicitrate iron acquisition and sugars metabolism. Features associated with cytotoxicity or intra-cellular survival were found specifically in the genomes of strains from severe and acute (VL2874) or persistent (VL2732) mastitis, respectively. MPEC genomes were relatively similar to strain K-12, which was subsequently shown here to be possibly pathogenic in the mammary gland. Phenome analysis showed that the persistent MPEC was the most versatile in terms of nutrients metabolized and acute MPEC the least. Among phenotypes unique to MPEC compared to the non-mammary pathogenic strain were uric acid and D-serine metabolism. This study reveals virulence factors and phenotypic characteristics of MPEC that may play a role in pathogenesis of E. coli mastitis.

No MeSH data available.


Related in: MedlinePlus