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Transgenic Arabidopsis Plants Expressing Tomato Glutathione S-Transferase Showed Enhanced Resistance to Salt and Drought Stress.

Xu J, Xing XJ, Tian YS, Peng RH, Xue Y, Zhao W, Yao QH - PLoS ONE (2015)

Bottom Line: Transgenic Arabidopsis plants were normal in terms of growth and maturity compared with wild-type plants.The increased tolerance of transgenic plants was correlated with the changes in proline, malondialdehyde and antioxidative emzymes activities.Our results indicated that the gene from tomato plays a positive role in improving tolerance to salinity and drought stresses in Arabidopsis.

View Article: PubMed Central - PubMed

Affiliation: Biotechnology Research Institute, Shanghai Academy of Agricultural Sciences, Shanghai, 201106, China.

ABSTRACT
Although glutathione S-transferases (GST, EC 2.5.1.18) are involved in response to abiotic stress, limited information is available regarding gene function in tomato. In this study, a GST gene from tomato, designated LeGSTU2, was cloned and functionally characterized. Expression profile analysis results showed that it was expressed in roots and flowers, and the transcription was induced by salt, osmotic, and heat stress. The gene was then introduced to Arabidopsis by Agrobacterium tumefaciens-mediated transformation. Transgenic Arabidopsis plants were normal in terms of growth and maturity compared with wild-type plants. Transgenic plants also showed an enhanced resistance to salt and osmotic stress induced by NaCl and mannitol. The increased tolerance of transgenic plants was correlated with the changes in proline, malondialdehyde and antioxidative emzymes activities. Our results indicated that the gene from tomato plays a positive role in improving tolerance to salinity and drought stresses in Arabidopsis.

No MeSH data available.


Related in: MedlinePlus

Changes in the content of proline, MDA, total chlorophyll content and the activities of SOD, POD and GST with different lines treated with NaCl (250mM) as salt stress or mannitol (300mM) as osmotic stress for 5d.a. proline content. b. MDA content. c. Total chlorophyll content. d. SOD activity. e. POD activity. f. GST activity. Values are mean±SD (n = 3), each from ten plants. The statistical significance was determined by Duncan’s multiple comparison tests. Different letters above bars indicate significant differences (P < 0.05) among different lines in the same treatment.
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pone.0136960.g007: Changes in the content of proline, MDA, total chlorophyll content and the activities of SOD, POD and GST with different lines treated with NaCl (250mM) as salt stress or mannitol (300mM) as osmotic stress for 5d.a. proline content. b. MDA content. c. Total chlorophyll content. d. SOD activity. e. POD activity. f. GST activity. Values are mean±SD (n = 3), each from ten plants. The statistical significance was determined by Duncan’s multiple comparison tests. Different letters above bars indicate significant differences (P < 0.05) among different lines in the same treatment.

Mentions: Proline, MDA and chlorophyll contents were analyzed (Fig 7A–7C) and the results showed that there were no difference between transgenic plants and WT. Under stress condition, proline and total chlorophyll contents increased in both transgenic plants and WT. However, the contents of proline and chlorophyll were significantly higher in transgenic plants than in WT plants during NaCl and mannitol stresses. The MDA content was also increased in both genotypes under stress condition, and WT showed significantly higher MDA content than that of transgenic plants, which means WT plants exhibited higher rates of cell damage than transgenic lines under stress conditions.


Transgenic Arabidopsis Plants Expressing Tomato Glutathione S-Transferase Showed Enhanced Resistance to Salt and Drought Stress.

Xu J, Xing XJ, Tian YS, Peng RH, Xue Y, Zhao W, Yao QH - PLoS ONE (2015)

Changes in the content of proline, MDA, total chlorophyll content and the activities of SOD, POD and GST with different lines treated with NaCl (250mM) as salt stress or mannitol (300mM) as osmotic stress for 5d.a. proline content. b. MDA content. c. Total chlorophyll content. d. SOD activity. e. POD activity. f. GST activity. Values are mean±SD (n = 3), each from ten plants. The statistical significance was determined by Duncan’s multiple comparison tests. Different letters above bars indicate significant differences (P < 0.05) among different lines in the same treatment.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4556630&req=5

pone.0136960.g007: Changes in the content of proline, MDA, total chlorophyll content and the activities of SOD, POD and GST with different lines treated with NaCl (250mM) as salt stress or mannitol (300mM) as osmotic stress for 5d.a. proline content. b. MDA content. c. Total chlorophyll content. d. SOD activity. e. POD activity. f. GST activity. Values are mean±SD (n = 3), each from ten plants. The statistical significance was determined by Duncan’s multiple comparison tests. Different letters above bars indicate significant differences (P < 0.05) among different lines in the same treatment.
Mentions: Proline, MDA and chlorophyll contents were analyzed (Fig 7A–7C) and the results showed that there were no difference between transgenic plants and WT. Under stress condition, proline and total chlorophyll contents increased in both transgenic plants and WT. However, the contents of proline and chlorophyll were significantly higher in transgenic plants than in WT plants during NaCl and mannitol stresses. The MDA content was also increased in both genotypes under stress condition, and WT showed significantly higher MDA content than that of transgenic plants, which means WT plants exhibited higher rates of cell damage than transgenic lines under stress conditions.

Bottom Line: Transgenic Arabidopsis plants were normal in terms of growth and maturity compared with wild-type plants.The increased tolerance of transgenic plants was correlated with the changes in proline, malondialdehyde and antioxidative emzymes activities.Our results indicated that the gene from tomato plays a positive role in improving tolerance to salinity and drought stresses in Arabidopsis.

View Article: PubMed Central - PubMed

Affiliation: Biotechnology Research Institute, Shanghai Academy of Agricultural Sciences, Shanghai, 201106, China.

ABSTRACT
Although glutathione S-transferases (GST, EC 2.5.1.18) are involved in response to abiotic stress, limited information is available regarding gene function in tomato. In this study, a GST gene from tomato, designated LeGSTU2, was cloned and functionally characterized. Expression profile analysis results showed that it was expressed in roots and flowers, and the transcription was induced by salt, osmotic, and heat stress. The gene was then introduced to Arabidopsis by Agrobacterium tumefaciens-mediated transformation. Transgenic Arabidopsis plants were normal in terms of growth and maturity compared with wild-type plants. Transgenic plants also showed an enhanced resistance to salt and osmotic stress induced by NaCl and mannitol. The increased tolerance of transgenic plants was correlated with the changes in proline, malondialdehyde and antioxidative emzymes activities. Our results indicated that the gene from tomato plays a positive role in improving tolerance to salinity and drought stresses in Arabidopsis.

No MeSH data available.


Related in: MedlinePlus