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Transgenic Arabidopsis Plants Expressing Tomato Glutathione S-Transferase Showed Enhanced Resistance to Salt and Drought Stress.

Xu J, Xing XJ, Tian YS, Peng RH, Xue Y, Zhao W, Yao QH - PLoS ONE (2015)

Bottom Line: Transgenic Arabidopsis plants were normal in terms of growth and maturity compared with wild-type plants.The increased tolerance of transgenic plants was correlated with the changes in proline, malondialdehyde and antioxidative emzymes activities.Our results indicated that the gene from tomato plays a positive role in improving tolerance to salinity and drought stresses in Arabidopsis.

View Article: PubMed Central - PubMed

Affiliation: Biotechnology Research Institute, Shanghai Academy of Agricultural Sciences, Shanghai, 201106, China.

ABSTRACT
Although glutathione S-transferases (GST, EC 2.5.1.18) are involved in response to abiotic stress, limited information is available regarding gene function in tomato. In this study, a GST gene from tomato, designated LeGSTU2, was cloned and functionally characterized. Expression profile analysis results showed that it was expressed in roots and flowers, and the transcription was induced by salt, osmotic, and heat stress. The gene was then introduced to Arabidopsis by Agrobacterium tumefaciens-mediated transformation. Transgenic Arabidopsis plants were normal in terms of growth and maturity compared with wild-type plants. Transgenic plants also showed an enhanced resistance to salt and osmotic stress induced by NaCl and mannitol. The increased tolerance of transgenic plants was correlated with the changes in proline, malondialdehyde and antioxidative emzymes activities. Our results indicated that the gene from tomato plays a positive role in improving tolerance to salinity and drought stresses in Arabidopsis.

No MeSH data available.


Related in: MedlinePlus

Stress tolerance of transgenic plants.a Three-week-old plants with different transgenic lines (OE3, OE7 and OE10) were given salt and water deficit stress treatment. Control, 3-week-old plants growing under normal conditions. Salt stress, plants watered with NaCl (250 mM). Water deficit stress, plants withheld watering for 2 weeks, then irrigated again. The experiment was replicated three times with similar results and for 48 plants per line for each experiment. b, Survival rate of different lines under stress treatment mentioned in a. Values are mean±SD of three individual experiments. The statistical significance was done and determined by Duncan’s multiple comparison tests. Different letters above bars indicate significant differences (P < 0.05) among different genotypes in the same treatment.
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pone.0136960.g005: Stress tolerance of transgenic plants.a Three-week-old plants with different transgenic lines (OE3, OE7 and OE10) were given salt and water deficit stress treatment. Control, 3-week-old plants growing under normal conditions. Salt stress, plants watered with NaCl (250 mM). Water deficit stress, plants withheld watering for 2 weeks, then irrigated again. The experiment was replicated three times with similar results and for 48 plants per line for each experiment. b, Survival rate of different lines under stress treatment mentioned in a. Values are mean±SD of three individual experiments. The statistical significance was done and determined by Duncan’s multiple comparison tests. Different letters above bars indicate significant differences (P < 0.05) among different genotypes in the same treatment.

Mentions: Three-week-old seedlings were watered with NaCl (250 mM) for salt stress, or with withheld watering for water deficit stress to further analyze salt/drought tolerance. After two weeks, some plants exhibited lethal effects, then the plants were irrigated again. The plants were photographed at 15 d after these plants were exposed to stress, the survival rate was then measured (Fig 5). Under salt stress condition, >80% of the transgenic plants survived, whereas only 20% of WT survived. Under water deficit stress for two weeks, >80% of the transgenic plants survived, whereas approximately 20% of WT plants survived.


Transgenic Arabidopsis Plants Expressing Tomato Glutathione S-Transferase Showed Enhanced Resistance to Salt and Drought Stress.

Xu J, Xing XJ, Tian YS, Peng RH, Xue Y, Zhao W, Yao QH - PLoS ONE (2015)

Stress tolerance of transgenic plants.a Three-week-old plants with different transgenic lines (OE3, OE7 and OE10) were given salt and water deficit stress treatment. Control, 3-week-old plants growing under normal conditions. Salt stress, plants watered with NaCl (250 mM). Water deficit stress, plants withheld watering for 2 weeks, then irrigated again. The experiment was replicated three times with similar results and for 48 plants per line for each experiment. b, Survival rate of different lines under stress treatment mentioned in a. Values are mean±SD of three individual experiments. The statistical significance was done and determined by Duncan’s multiple comparison tests. Different letters above bars indicate significant differences (P < 0.05) among different genotypes in the same treatment.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4556630&req=5

pone.0136960.g005: Stress tolerance of transgenic plants.a Three-week-old plants with different transgenic lines (OE3, OE7 and OE10) were given salt and water deficit stress treatment. Control, 3-week-old plants growing under normal conditions. Salt stress, plants watered with NaCl (250 mM). Water deficit stress, plants withheld watering for 2 weeks, then irrigated again. The experiment was replicated three times with similar results and for 48 plants per line for each experiment. b, Survival rate of different lines under stress treatment mentioned in a. Values are mean±SD of three individual experiments. The statistical significance was done and determined by Duncan’s multiple comparison tests. Different letters above bars indicate significant differences (P < 0.05) among different genotypes in the same treatment.
Mentions: Three-week-old seedlings were watered with NaCl (250 mM) for salt stress, or with withheld watering for water deficit stress to further analyze salt/drought tolerance. After two weeks, some plants exhibited lethal effects, then the plants were irrigated again. The plants were photographed at 15 d after these plants were exposed to stress, the survival rate was then measured (Fig 5). Under salt stress condition, >80% of the transgenic plants survived, whereas only 20% of WT survived. Under water deficit stress for two weeks, >80% of the transgenic plants survived, whereas approximately 20% of WT plants survived.

Bottom Line: Transgenic Arabidopsis plants were normal in terms of growth and maturity compared with wild-type plants.The increased tolerance of transgenic plants was correlated with the changes in proline, malondialdehyde and antioxidative emzymes activities.Our results indicated that the gene from tomato plays a positive role in improving tolerance to salinity and drought stresses in Arabidopsis.

View Article: PubMed Central - PubMed

Affiliation: Biotechnology Research Institute, Shanghai Academy of Agricultural Sciences, Shanghai, 201106, China.

ABSTRACT
Although glutathione S-transferases (GST, EC 2.5.1.18) are involved in response to abiotic stress, limited information is available regarding gene function in tomato. In this study, a GST gene from tomato, designated LeGSTU2, was cloned and functionally characterized. Expression profile analysis results showed that it was expressed in roots and flowers, and the transcription was induced by salt, osmotic, and heat stress. The gene was then introduced to Arabidopsis by Agrobacterium tumefaciens-mediated transformation. Transgenic Arabidopsis plants were normal in terms of growth and maturity compared with wild-type plants. Transgenic plants also showed an enhanced resistance to salt and osmotic stress induced by NaCl and mannitol. The increased tolerance of transgenic plants was correlated with the changes in proline, malondialdehyde and antioxidative emzymes activities. Our results indicated that the gene from tomato plays a positive role in improving tolerance to salinity and drought stresses in Arabidopsis.

No MeSH data available.


Related in: MedlinePlus