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Transgenic Arabidopsis Plants Expressing Tomato Glutathione S-Transferase Showed Enhanced Resistance to Salt and Drought Stress.

Xu J, Xing XJ, Tian YS, Peng RH, Xue Y, Zhao W, Yao QH - PLoS ONE (2015)

Bottom Line: Transgenic Arabidopsis plants were normal in terms of growth and maturity compared with wild-type plants.The increased tolerance of transgenic plants was correlated with the changes in proline, malondialdehyde and antioxidative emzymes activities.Our results indicated that the gene from tomato plays a positive role in improving tolerance to salinity and drought stresses in Arabidopsis.

View Article: PubMed Central - PubMed

Affiliation: Biotechnology Research Institute, Shanghai Academy of Agricultural Sciences, Shanghai, 201106, China.

ABSTRACT
Although glutathione S-transferases (GST, EC 2.5.1.18) are involved in response to abiotic stress, limited information is available regarding gene function in tomato. In this study, a GST gene from tomato, designated LeGSTU2, was cloned and functionally characterized. Expression profile analysis results showed that it was expressed in roots and flowers, and the transcription was induced by salt, osmotic, and heat stress. The gene was then introduced to Arabidopsis by Agrobacterium tumefaciens-mediated transformation. Transgenic Arabidopsis plants were normal in terms of growth and maturity compared with wild-type plants. Transgenic plants also showed an enhanced resistance to salt and osmotic stress induced by NaCl and mannitol. The increased tolerance of transgenic plants was correlated with the changes in proline, malondialdehyde and antioxidative emzymes activities. Our results indicated that the gene from tomato plays a positive role in improving tolerance to salinity and drought stresses in Arabidopsis.

No MeSH data available.


Related in: MedlinePlus

Expression profile of LeGSTU2 in tomato.a, The expression of LeGSTU2 in different organs by real-time PCR. Total RNA was isolated from different organization (roots, stems, leaves, flowers or fruits) of tomato seedlings. The experiment was replicated three times and the mean value is shown. b, Expression of LeGSTU2 transcripts in response to drought, salt, cold and heat stresses by real-time PCR. Total RNA was isolated from tomato seedlings, which was exposed to different stress at indicated times (0 h as a control). 200 mM NaCl for salt stress, 50mM Mannitol for osmotic stress, 40°C for heat stress and 4°C for cold stress. The experiment was replicated three times and the mean value is shown.
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pone.0136960.g002: Expression profile of LeGSTU2 in tomato.a, The expression of LeGSTU2 in different organs by real-time PCR. Total RNA was isolated from different organization (roots, stems, leaves, flowers or fruits) of tomato seedlings. The experiment was replicated three times and the mean value is shown. b, Expression of LeGSTU2 transcripts in response to drought, salt, cold and heat stresses by real-time PCR. Total RNA was isolated from tomato seedlings, which was exposed to different stress at indicated times (0 h as a control). 200 mM NaCl for salt stress, 50mM Mannitol for osmotic stress, 40°C for heat stress and 4°C for cold stress. The experiment was replicated three times and the mean value is shown.

Mentions: Different organs of tomato seedlings were harvested, and the expression of LeGSTU2 was analyzed by real-time PCR to examine the expression profile. The results showed that LeGSTU2 was highly expressed in roots and flowers, suggesting that LeGSTU2 could participate in root growth and flower development (Fig 2A).


Transgenic Arabidopsis Plants Expressing Tomato Glutathione S-Transferase Showed Enhanced Resistance to Salt and Drought Stress.

Xu J, Xing XJ, Tian YS, Peng RH, Xue Y, Zhao W, Yao QH - PLoS ONE (2015)

Expression profile of LeGSTU2 in tomato.a, The expression of LeGSTU2 in different organs by real-time PCR. Total RNA was isolated from different organization (roots, stems, leaves, flowers or fruits) of tomato seedlings. The experiment was replicated three times and the mean value is shown. b, Expression of LeGSTU2 transcripts in response to drought, salt, cold and heat stresses by real-time PCR. Total RNA was isolated from tomato seedlings, which was exposed to different stress at indicated times (0 h as a control). 200 mM NaCl for salt stress, 50mM Mannitol for osmotic stress, 40°C for heat stress and 4°C for cold stress. The experiment was replicated three times and the mean value is shown.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4556630&req=5

pone.0136960.g002: Expression profile of LeGSTU2 in tomato.a, The expression of LeGSTU2 in different organs by real-time PCR. Total RNA was isolated from different organization (roots, stems, leaves, flowers or fruits) of tomato seedlings. The experiment was replicated three times and the mean value is shown. b, Expression of LeGSTU2 transcripts in response to drought, salt, cold and heat stresses by real-time PCR. Total RNA was isolated from tomato seedlings, which was exposed to different stress at indicated times (0 h as a control). 200 mM NaCl for salt stress, 50mM Mannitol for osmotic stress, 40°C for heat stress and 4°C for cold stress. The experiment was replicated three times and the mean value is shown.
Mentions: Different organs of tomato seedlings were harvested, and the expression of LeGSTU2 was analyzed by real-time PCR to examine the expression profile. The results showed that LeGSTU2 was highly expressed in roots and flowers, suggesting that LeGSTU2 could participate in root growth and flower development (Fig 2A).

Bottom Line: Transgenic Arabidopsis plants were normal in terms of growth and maturity compared with wild-type plants.The increased tolerance of transgenic plants was correlated with the changes in proline, malondialdehyde and antioxidative emzymes activities.Our results indicated that the gene from tomato plays a positive role in improving tolerance to salinity and drought stresses in Arabidopsis.

View Article: PubMed Central - PubMed

Affiliation: Biotechnology Research Institute, Shanghai Academy of Agricultural Sciences, Shanghai, 201106, China.

ABSTRACT
Although glutathione S-transferases (GST, EC 2.5.1.18) are involved in response to abiotic stress, limited information is available regarding gene function in tomato. In this study, a GST gene from tomato, designated LeGSTU2, was cloned and functionally characterized. Expression profile analysis results showed that it was expressed in roots and flowers, and the transcription was induced by salt, osmotic, and heat stress. The gene was then introduced to Arabidopsis by Agrobacterium tumefaciens-mediated transformation. Transgenic Arabidopsis plants were normal in terms of growth and maturity compared with wild-type plants. Transgenic plants also showed an enhanced resistance to salt and osmotic stress induced by NaCl and mannitol. The increased tolerance of transgenic plants was correlated with the changes in proline, malondialdehyde and antioxidative emzymes activities. Our results indicated that the gene from tomato plays a positive role in improving tolerance to salinity and drought stresses in Arabidopsis.

No MeSH data available.


Related in: MedlinePlus