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Transgenic Arabidopsis Plants Expressing Tomato Glutathione S-Transferase Showed Enhanced Resistance to Salt and Drought Stress.

Xu J, Xing XJ, Tian YS, Peng RH, Xue Y, Zhao W, Yao QH - PLoS ONE (2015)

Bottom Line: Transgenic Arabidopsis plants were normal in terms of growth and maturity compared with wild-type plants.The increased tolerance of transgenic plants was correlated with the changes in proline, malondialdehyde and antioxidative emzymes activities.Our results indicated that the gene from tomato plays a positive role in improving tolerance to salinity and drought stresses in Arabidopsis.

View Article: PubMed Central - PubMed

Affiliation: Biotechnology Research Institute, Shanghai Academy of Agricultural Sciences, Shanghai, 201106, China.

ABSTRACT
Although glutathione S-transferases (GST, EC 2.5.1.18) are involved in response to abiotic stress, limited information is available regarding gene function in tomato. In this study, a GST gene from tomato, designated LeGSTU2, was cloned and functionally characterized. Expression profile analysis results showed that it was expressed in roots and flowers, and the transcription was induced by salt, osmotic, and heat stress. The gene was then introduced to Arabidopsis by Agrobacterium tumefaciens-mediated transformation. Transgenic Arabidopsis plants were normal in terms of growth and maturity compared with wild-type plants. Transgenic plants also showed an enhanced resistance to salt and osmotic stress induced by NaCl and mannitol. The increased tolerance of transgenic plants was correlated with the changes in proline, malondialdehyde and antioxidative emzymes activities. Our results indicated that the gene from tomato plays a positive role in improving tolerance to salinity and drought stresses in Arabidopsis.

No MeSH data available.


Related in: MedlinePlus

Phylogenetic tree for the amino acid sequences of LeGSTU2 with the GST proteins from Arabidopsis thaliana.The NJ method was applied using Mega 4 from a tree file produced by Clustal W2.
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pone.0136960.g001: Phylogenetic tree for the amino acid sequences of LeGSTU2 with the GST proteins from Arabidopsis thaliana.The NJ method was applied using Mega 4 from a tree file produced by Clustal W2.

Mentions: The coding region of LeGSTU2 was 663 bp in length and was predicted to encode a 220 amino acid protein with a calculated MW of 25.4 kDa and a pI of 5.51. Multiple sequence alignments showed that LeGSTU2 share the highest similarity with AtGSTU19 (At1g78380), AtGSTU6 (At2g29440), AtGSTU2 (At2g29480) and AtGSTU4 (At2g29460), which suggests that LeGSTU2 may be a lambda-type GST (Fig 1).


Transgenic Arabidopsis Plants Expressing Tomato Glutathione S-Transferase Showed Enhanced Resistance to Salt and Drought Stress.

Xu J, Xing XJ, Tian YS, Peng RH, Xue Y, Zhao W, Yao QH - PLoS ONE (2015)

Phylogenetic tree for the amino acid sequences of LeGSTU2 with the GST proteins from Arabidopsis thaliana.The NJ method was applied using Mega 4 from a tree file produced by Clustal W2.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4556630&req=5

pone.0136960.g001: Phylogenetic tree for the amino acid sequences of LeGSTU2 with the GST proteins from Arabidopsis thaliana.The NJ method was applied using Mega 4 from a tree file produced by Clustal W2.
Mentions: The coding region of LeGSTU2 was 663 bp in length and was predicted to encode a 220 amino acid protein with a calculated MW of 25.4 kDa and a pI of 5.51. Multiple sequence alignments showed that LeGSTU2 share the highest similarity with AtGSTU19 (At1g78380), AtGSTU6 (At2g29440), AtGSTU2 (At2g29480) and AtGSTU4 (At2g29460), which suggests that LeGSTU2 may be a lambda-type GST (Fig 1).

Bottom Line: Transgenic Arabidopsis plants were normal in terms of growth and maturity compared with wild-type plants.The increased tolerance of transgenic plants was correlated with the changes in proline, malondialdehyde and antioxidative emzymes activities.Our results indicated that the gene from tomato plays a positive role in improving tolerance to salinity and drought stresses in Arabidopsis.

View Article: PubMed Central - PubMed

Affiliation: Biotechnology Research Institute, Shanghai Academy of Agricultural Sciences, Shanghai, 201106, China.

ABSTRACT
Although glutathione S-transferases (GST, EC 2.5.1.18) are involved in response to abiotic stress, limited information is available regarding gene function in tomato. In this study, a GST gene from tomato, designated LeGSTU2, was cloned and functionally characterized. Expression profile analysis results showed that it was expressed in roots and flowers, and the transcription was induced by salt, osmotic, and heat stress. The gene was then introduced to Arabidopsis by Agrobacterium tumefaciens-mediated transformation. Transgenic Arabidopsis plants were normal in terms of growth and maturity compared with wild-type plants. Transgenic plants also showed an enhanced resistance to salt and osmotic stress induced by NaCl and mannitol. The increased tolerance of transgenic plants was correlated with the changes in proline, malondialdehyde and antioxidative emzymes activities. Our results indicated that the gene from tomato plays a positive role in improving tolerance to salinity and drought stresses in Arabidopsis.

No MeSH data available.


Related in: MedlinePlus