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Scaffold-Free Tubular Tissues Created by a Bio-3D Printer Undergo Remodeling and Endothelialization when Implanted in Rat Aortae.

Itoh M, Nakayama K, Noguchi R, Kamohara K, Furukawa K, Uchihashi K, Toda S, Oyama J, Node K, Morita S - PLoS ONE (2015)

Bottom Line: All grafts were patent and remodeling of the tubular tissues (enlargement of the lumen area and thinning of the wall) was observed.A layer of endothelial cells was confirmed five days after implantation.Further studies are warranted to elucidate the underlying mechanism of endothelialization and its function, as well as the long-term results.

View Article: PubMed Central - PubMed

Affiliation: Department of Thoracic and Cardiovascular Surgery, Faculty of Medicine, Saga University, Saga, Japan.

ABSTRACT

Background: Small caliber vascular prostheses are not clinically available because synthetic vascular prostheses lack endothelial cells which modulate platelet activation, leukocyte adhesion, thrombosis, and the regulation of vasomotor tone by the production of vasoactive substances. We developed a novel method to create scaffold-free tubular tissue from multicellular spheroids (MCS) using a "Bio-3D printer"-based system. This system enables the creation of pre-designed three-dimensional structures using a computer controlled robotics system. With this system, we created a tubular structure and studied its biological features.

Methods and results: Using a "Bio-3D printer," we made scaffold-free tubular tissues (inner diameter of 1.5 mm) from a total of 500 MCSs (2.5× 104 cells per one MCS) composed of human umbilical vein endothelial cells (40%), human aortic smooth muscle cells (10%), and normal human dermal fibroblasts (50%). The tubular tissues were cultured in a perfusion system and implanted into the abdominal aortas of F344 nude rats. We assessed the flow by ultrasonography and performed histological examinations on the second (n = 5) and fifth (n = 5) day after implantation. All grafts were patent and remodeling of the tubular tissues (enlargement of the lumen area and thinning of the wall) was observed. A layer of endothelial cells was confirmed five days after implantation.

Conclusions: The scaffold-free tubular tissues made of MCS using a Bio-3D printer underwent remodeling and endothelialization. Further studies are warranted to elucidate the underlying mechanism of endothelialization and its function, as well as the long-term results.

No MeSH data available.


Related in: MedlinePlus

The rat aortae were stained with HE and CD31, respectively (A).The number of endothelial cells in the five rat aortae was counted and compared with those in the tubular tissues (B).
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pone.0136681.g008: The rat aortae were stained with HE and CD31, respectively (A).The number of endothelial cells in the five rat aortae was counted and compared with those in the tubular tissues (B).

Mentions: The lumen area and the wall area of the tubular tissue were measured before and five days after implantation (Fig 8A and 8B). The lumen area was enlarged (P = 0.032) and the wall area was decreased (P = 0.008) after implantation. The total area (the sum of the wall area and lumen area) showed no significant difference (Fig 8C).


Scaffold-Free Tubular Tissues Created by a Bio-3D Printer Undergo Remodeling and Endothelialization when Implanted in Rat Aortae.

Itoh M, Nakayama K, Noguchi R, Kamohara K, Furukawa K, Uchihashi K, Toda S, Oyama J, Node K, Morita S - PLoS ONE (2015)

The rat aortae were stained with HE and CD31, respectively (A).The number of endothelial cells in the five rat aortae was counted and compared with those in the tubular tissues (B).
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4556622&req=5

pone.0136681.g008: The rat aortae were stained with HE and CD31, respectively (A).The number of endothelial cells in the five rat aortae was counted and compared with those in the tubular tissues (B).
Mentions: The lumen area and the wall area of the tubular tissue were measured before and five days after implantation (Fig 8A and 8B). The lumen area was enlarged (P = 0.032) and the wall area was decreased (P = 0.008) after implantation. The total area (the sum of the wall area and lumen area) showed no significant difference (Fig 8C).

Bottom Line: All grafts were patent and remodeling of the tubular tissues (enlargement of the lumen area and thinning of the wall) was observed.A layer of endothelial cells was confirmed five days after implantation.Further studies are warranted to elucidate the underlying mechanism of endothelialization and its function, as well as the long-term results.

View Article: PubMed Central - PubMed

Affiliation: Department of Thoracic and Cardiovascular Surgery, Faculty of Medicine, Saga University, Saga, Japan.

ABSTRACT

Background: Small caliber vascular prostheses are not clinically available because synthetic vascular prostheses lack endothelial cells which modulate platelet activation, leukocyte adhesion, thrombosis, and the regulation of vasomotor tone by the production of vasoactive substances. We developed a novel method to create scaffold-free tubular tissue from multicellular spheroids (MCS) using a "Bio-3D printer"-based system. This system enables the creation of pre-designed three-dimensional structures using a computer controlled robotics system. With this system, we created a tubular structure and studied its biological features.

Methods and results: Using a "Bio-3D printer," we made scaffold-free tubular tissues (inner diameter of 1.5 mm) from a total of 500 MCSs (2.5× 104 cells per one MCS) composed of human umbilical vein endothelial cells (40%), human aortic smooth muscle cells (10%), and normal human dermal fibroblasts (50%). The tubular tissues were cultured in a perfusion system and implanted into the abdominal aortas of F344 nude rats. We assessed the flow by ultrasonography and performed histological examinations on the second (n = 5) and fifth (n = 5) day after implantation. All grafts were patent and remodeling of the tubular tissues (enlargement of the lumen area and thinning of the wall) was observed. A layer of endothelial cells was confirmed five days after implantation.

Conclusions: The scaffold-free tubular tissues made of MCS using a Bio-3D printer underwent remodeling and endothelialization. Further studies are warranted to elucidate the underlying mechanism of endothelialization and its function, as well as the long-term results.

No MeSH data available.


Related in: MedlinePlus