Limits...
Genotype Distribution and Molecular Epidemiology of Hepatitis C Virus in Hubei, Central China.

Peng J, Lu Y, Liu W, Zhu Y, Yan X, Xu J, Wang X, Wang Y, Liu W, Sun Z - PLoS ONE (2015)

Bottom Line: Analysis of genotype-associated risk factors revealed that paid blood donation and transfusion before 1997 were strongly associated with subtypes 1b and 2a, while some subtype 2a cases were also found in individuals with high risk sexual behaviors; subtypes 3b, 6a, and 3a were detected only in intravenous drug users.Among them, subtype 1b Hubei strains may have served as the origins of this subtype in China, and 2a and 3b Hubei strains may have descended from the northwest and southwest of China, respectively, while 6a Hubei strains may have been imported from the central south and southwest.The results suggest that the migration patterns of HCV in Hubei are complex and variable among different subtypes.

View Article: PubMed Central - PubMed

Affiliation: Department of Clinical Laboratory, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.

ABSTRACT

Background: Little is known about the molecular epidemiology of hepatitis C virus (HCV) infection in Central China.

Methodology/principal findings: A total of 570 patients from Hubei Province in central China were enrolled. These patients were tested positive for HCV antibody prior to blood transfusion. Among them, 177 were characterized by partial NS5B and/or Core-E1 sequences and classified into five subtypes: 1b, 83.0% (147/177); 2a, 13.0% (23/177); 3b, 2.3% (4/177); 6a, 1.1% (2/177); 3a, 0.6% (1/177). Analysis of genotype-associated risk factors revealed that paid blood donation and transfusion before 1997 were strongly associated with subtypes 1b and 2a, while some subtype 2a cases were also found in individuals with high risk sexual behaviors; subtypes 3b, 6a, and 3a were detected only in intravenous drug users. Phylogeographic analyses based on the coalescent datasets demonstrated that 1b, 2a, 3b, and 6a were locally epidemic in Hubei Province. Among them, subtype 1b Hubei strains may have served as the origins of this subtype in China, and 2a and 3b Hubei strains may have descended from the northwest and southwest of China, respectively, while 6a Hubei strains may have been imported from the central south and southwest.

Conclusion/significance: The results suggest that the migration patterns of HCV in Hubei are complex and variable among different subtypes. Implementation of mandatory HCV screening before donation has significantly decreased the incidence of transfusion-associated HCV infection since 1997. More attention should be paid to intravenous drug use and unsafe sexual contact, which may have become new risk factors for HCV infection in Hubei Province.

No MeSH data available.


Related in: MedlinePlus

Phylogeographic tree generated with the partial Core-E1 sequences of subtype 6a isolates.A total of 103 subtype 2a sequences were used to reconstruct the phylogeographic tree: 2 obtained in this study, 29 from IDUs in Wuhan City [14], 46 from blood donors from the 17 provinces and municipalities in China, including 1 Hubei sequence [5], 4 from IDUs in Yunnan Province, and 22 from Vietnamese IDUs. The indications here are the same as those in Fig 3.
© Copyright Policy
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4556612&req=5

pone.0137059.g006: Phylogeographic tree generated with the partial Core-E1 sequences of subtype 6a isolates.A total of 103 subtype 2a sequences were used to reconstruct the phylogeographic tree: 2 obtained in this study, 29 from IDUs in Wuhan City [14], 46 from blood donors from the 17 provinces and municipalities in China, including 1 Hubei sequence [5], 4 from IDUs in Yunnan Province, and 22 from Vietnamese IDUs. The indications here are the same as those in Fig 3.

Mentions: Fig 6 presents a phylogeographic tree reconstructed on the basis of the 103 Core-E1 sequences of subtype 6a. Overall, three subsets were visible, showing posterior probabilities of 0.88, 0.71, and 0.97, respectively. The upper subset featured sequences mainly from the central south and can be further divided into five groups. Among them four displayed full posterior probabilities of 1.00. The middle subset contained sequences exclusively from Vietnam and showed a posterior probability of 0.71. The lower one showed a posterior probability of 0.97 and contained sequences from three areas: Hubei, Yunnan, and Vietnam,. As a whole, there appeared a trend of 6a migration in China: descended from Vietnam, 6a was first introduced into Yunnan and Guangdong, and then from Guangdong to other regions. The Hubei strains seemed to have two ancestors, which were located in Guangdong and Yunnan, respectively. Since its introduction, 6a may have become local epidemics in Hubei, indicated by four clusters of Hubei sequences observed in the phylogeographic tree.


Genotype Distribution and Molecular Epidemiology of Hepatitis C Virus in Hubei, Central China.

Peng J, Lu Y, Liu W, Zhu Y, Yan X, Xu J, Wang X, Wang Y, Liu W, Sun Z - PLoS ONE (2015)

Phylogeographic tree generated with the partial Core-E1 sequences of subtype 6a isolates.A total of 103 subtype 2a sequences were used to reconstruct the phylogeographic tree: 2 obtained in this study, 29 from IDUs in Wuhan City [14], 46 from blood donors from the 17 provinces and municipalities in China, including 1 Hubei sequence [5], 4 from IDUs in Yunnan Province, and 22 from Vietnamese IDUs. The indications here are the same as those in Fig 3.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4556612&req=5

pone.0137059.g006: Phylogeographic tree generated with the partial Core-E1 sequences of subtype 6a isolates.A total of 103 subtype 2a sequences were used to reconstruct the phylogeographic tree: 2 obtained in this study, 29 from IDUs in Wuhan City [14], 46 from blood donors from the 17 provinces and municipalities in China, including 1 Hubei sequence [5], 4 from IDUs in Yunnan Province, and 22 from Vietnamese IDUs. The indications here are the same as those in Fig 3.
Mentions: Fig 6 presents a phylogeographic tree reconstructed on the basis of the 103 Core-E1 sequences of subtype 6a. Overall, three subsets were visible, showing posterior probabilities of 0.88, 0.71, and 0.97, respectively. The upper subset featured sequences mainly from the central south and can be further divided into five groups. Among them four displayed full posterior probabilities of 1.00. The middle subset contained sequences exclusively from Vietnam and showed a posterior probability of 0.71. The lower one showed a posterior probability of 0.97 and contained sequences from three areas: Hubei, Yunnan, and Vietnam,. As a whole, there appeared a trend of 6a migration in China: descended from Vietnam, 6a was first introduced into Yunnan and Guangdong, and then from Guangdong to other regions. The Hubei strains seemed to have two ancestors, which were located in Guangdong and Yunnan, respectively. Since its introduction, 6a may have become local epidemics in Hubei, indicated by four clusters of Hubei sequences observed in the phylogeographic tree.

Bottom Line: Analysis of genotype-associated risk factors revealed that paid blood donation and transfusion before 1997 were strongly associated with subtypes 1b and 2a, while some subtype 2a cases were also found in individuals with high risk sexual behaviors; subtypes 3b, 6a, and 3a were detected only in intravenous drug users.Among them, subtype 1b Hubei strains may have served as the origins of this subtype in China, and 2a and 3b Hubei strains may have descended from the northwest and southwest of China, respectively, while 6a Hubei strains may have been imported from the central south and southwest.The results suggest that the migration patterns of HCV in Hubei are complex and variable among different subtypes.

View Article: PubMed Central - PubMed

Affiliation: Department of Clinical Laboratory, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.

ABSTRACT

Background: Little is known about the molecular epidemiology of hepatitis C virus (HCV) infection in Central China.

Methodology/principal findings: A total of 570 patients from Hubei Province in central China were enrolled. These patients were tested positive for HCV antibody prior to blood transfusion. Among them, 177 were characterized by partial NS5B and/or Core-E1 sequences and classified into five subtypes: 1b, 83.0% (147/177); 2a, 13.0% (23/177); 3b, 2.3% (4/177); 6a, 1.1% (2/177); 3a, 0.6% (1/177). Analysis of genotype-associated risk factors revealed that paid blood donation and transfusion before 1997 were strongly associated with subtypes 1b and 2a, while some subtype 2a cases were also found in individuals with high risk sexual behaviors; subtypes 3b, 6a, and 3a were detected only in intravenous drug users. Phylogeographic analyses based on the coalescent datasets demonstrated that 1b, 2a, 3b, and 6a were locally epidemic in Hubei Province. Among them, subtype 1b Hubei strains may have served as the origins of this subtype in China, and 2a and 3b Hubei strains may have descended from the northwest and southwest of China, respectively, while 6a Hubei strains may have been imported from the central south and southwest.

Conclusion/significance: The results suggest that the migration patterns of HCV in Hubei are complex and variable among different subtypes. Implementation of mandatory HCV screening before donation has significantly decreased the incidence of transfusion-associated HCV infection since 1997. More attention should be paid to intravenous drug use and unsafe sexual contact, which may have become new risk factors for HCV infection in Hubei Province.

No MeSH data available.


Related in: MedlinePlus